EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:12142

Rtn3 reticulon 3 ( MGI:1339970)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008415. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008415_01	euxassay_008415_02	euxassay_008415_03	euxassay_008415_04

euxassay_008415_05	euxassay_008415_06	euxassay_008415_07	euxassay_008415_08	euxassay_008415_09

euxassay_008415_10	euxassay_008415_11	euxassay_008415_12	euxassay_008415_13	euxassay_008415_14

euxassay_008415_15	euxassay_008415_16	euxassay_008415_17	euxassay_008415_18	euxassay_008415_19

euxassay_008415_20	euxassay_008415_21	euxassay_008415_22	euxassay_008415_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12142_wholemount_strong.wlz

12142_wholemount_moderate.wlz

12142_wholemount_weak.wlz

12142_wholemount_possible.wlz

12142_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12142_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

vibrissa

strong

regional

strong expression: see section 09 10 11 21 22 23 moderate expression: see section 06 08

facial vii ganglion

strong

regional

strong expression: see section 05 06 07 08 20 21 22 23

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 08 09 19

trigeminal v ganglion

strong

regional

strong expression: see section 05 06 07 08 09 10 11 19 20 21 22 23

vagus x ganglion

strong

regional

strong expression: see section 09 19

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 07 08 09 10 18 19 20

trigeminal v nerve

strong

regional

strong expression: see section 11 12 18

spinal cord

strong

regional

strong expression: see section 09 10 11 12 13 14 15 16 17 18

cervico-thoracic ganglion

strong

regional

strong expression: see section 11 12 13 17

cervical ganglion

strong

regional

strong expression: see section 10 11 17 18

thoracic ganglion

strong

regional

strong expression: see section 13 14 15 16

dorsal root ganglion

strong

regional

strong expression: see section 08 09 10 11 12 17 18

neural retina

strong

regional

strong expression: see section 01 02 03 04 05 06

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 10 11 12 13 14 15 17 18 19 20 21

tongue

strong

spotted

strong expression: see section 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9283
Entity Detected:	Rtn3, reticulon 3 ( MGI:1339970)
Sequence:	sense strand is shown >T9283 TTCCGAACAGGCGTCGCCGGGAACTTCGGAGAGCCAGCTGCTGGATTATTCTGTGTCCCCTCCCACTCTC CCGCCCTACGTCTCTCTTCACCCTTCTCCCGCCCTTGCTCGTACAGCCATGGCGGAGTCGTCAGCGGCCA CTCAGTCCCCGTCAGTCTCCTCGTCGTCCTCCGGGGCCGAGCCGTCAGCTCTCGGCGGCGGCGGCGGGAG CCCTGGAGCCTGCCCCGCCCTGGGGGCGAAGAGCTGCGGCTCCTCGTGTGCGGATTCCTTTGTTTCTTCC TCTTCCTCTCAGCCTGTATCTATATTTTCGACCTCACAAGCGGGATTGAGCTCTCTTTGCTCTGATGAGC CACCTTCAAAAAGTATGACTTCCTCCTTTCTTTCATCTTCTGAAATACATAACCCTGACCCTACAACACC GCTTGGAGAAAAGAGTGAAACATTAGGTAGCCAGTTTGTTCTAGCTAAAGGAAAAGACCCCTTGGTTCTT CTAGATAAGAAAAAACTGGACTCACCTCAGGGGACCAACAAGGACAGAGTAGACGCTCCGGTTTCTCTTG CAACTGGCATTCCTTGCAGCCACCCTTCTATTCCAGACAGTTTCCCAGAGCAACCTGCTTTTCTGTCAAA AGAAATTGGTCCAGCAGAAGAGTGGGTAGTTAAAGACCAAGAACCCAAGAACCCAAACAAGGTTCCAGAT GGGGAGGACAGAAGTGCACTGGATTTTGGGCAGTCTAAGGCAGAACACATTTGTACATATTCCTTGTCCC CATCTGAACTTCCAGTAGCCAGTGTAGAAAAAGATTCTCCTGAGTCACCGTTTGAAGTAATTATTGACAA AGCAACATTTGACAGAGAATTTAAAGATTTGTATAAGGAAAACCCAAATGATTTGGGTGGCTGGGCAGCT CATGGCGATAGAGAATCCCCCGCAGACTTGTTGGAAATGAATGACAAACTCTTTCCACTGAGAAATAAAG AGGCAGGGCGTTACCCATCCTCTGTACTGCTCGGTAGACAGTTCTCACACACTACAGCAGCACTGGAAGA GGTGTCTAGATGTGTGAATGATATGCATAACTTTACTAATGAAATACTGACTTGGGACTTAGATCCCCAA GCAAAACAACAGGCCAATAAAACATCTTGCACCACAGAAAGTACAGGACTAGACAGGAGTGAACTTCGCT CAGAAATTCCAGTTATAAATCTTAAAACAAACCCTCAACAGAAAATGCCTGTATGTTCTTTTAATGGGAG CACTCCCATTACCAAATCCACAGGTGACTGGACAGAAGCATTTACAGAAGGAAAACCTGTAAGAGACTAC CTCAGTTCCACAAAAGAAGCTGGTGGCAACGGTGTGCCAGGCAGTTCTCAGCTTCATTCTGAGCTGCCTG GCTCTATGCCTGAGAAATGGGTCTCAGGCTCTGGAGCAGCCACAGTGGAAGTAACTTTACCTAACCTGAG GGGTGCCTGGCCTAACTCTGTGATGGGGGAAGTCACAGAGGTTGATAGTTCTGGGGAATCTGATGACACA GTAATAGAGGACATCACAGAAAAACCTGACTCCCTTCCAAGTGCTGCTGCAAAAACAAGTGAAAGGGAAA TCAAAGAGACTCCCAGTCGTGAAACTGTGAGGAGTGAAATGTGTGAAAACTCTGAGCAGCCGCAGGCCCA GCCAGAAACTCCTACTCAGAAGAGTCTGGAAGGTGAGGTGGCTTCACAAGTACCTAATACCCTGAATGAA GTCACACCTGAAAAGCTTGATATGACTAACAACCCCAAAGTTTGCTCAGCAGCACCTCCAAGTGTTCTTA ATGAGACAGGATTCTCACTAACTGTGCCAGCTTCTGCCAAGTTGGAATCTTTGCTTGGAAAATATGTTGA AGATACAGATGGTTCCTCCCCAGAGGACTTGATGGCTGTCCTCACAGGAGCCGAGGAGAAGGGGATAGTG GATAAAGAGGAAGGTGATGTTTTGGAAGCAGTGTTAGAGAAGATAGCAGACTTTAAAAACACTTTGCCTG TGGAACTCTTGCATGAAAGTGAGCTAAGTGGTTCTGAAACCAAAAATATTAAAAGCAAATACAGTGAAGA CAGCAGAGAAACAACTGGAGGTGCCCCTACGATGTCTCCCGACTTAGAGCAGGAGCAGCTCACCATCAGA GCCATTAAAGAATTAGGAGAAAGGCAAGCTGAGAAGGTGCAGGATGAAGGAATATCTTCTGGAGGAAAAC TCAAGCAAACCTTTGCTCCACAGTCTGGGCCACAGAGTTCATCTGACATCCTAGAACACACAGATGTCAA AACTGGATCTGATCTTGGAATTCCCAAAAATCCTACTATCATCAAAAACACTAGAATAGATTCTATTTCC AGCCTTACCAAGACTGAAATGGTTAACAAGAATGTTCTAGCAAGACTTCTCAGTGATTTCCCAGTGCACG ATCTGATTTTCTGGCGAGATGTGAAGAAGACTGGGTTTGTCTTTGGCACCACACTGATCATGCTGCTCTC TCTGGCAGCCTTCAGTGTTATCAGTGTGGTCTCTTACCTCATCCTGGCTCTTCTCTCTGTCACCATCAGC TTCAGAGTCTATAAGTCTGTCATTCAAGCTGTGCAGAAGTCAGAAGAAGGACATCCATTCAAGGCCTACT TGGATGTGGACATTACCCTGTCTTCAGAAGCTTTCCACAACTACATGAATGCTGCGATGGTGCATGTCAA CAAGGCCCTCAAACTCATTATTCGTCTCTTTCTGGTAGAAGACTTGGTTGACTCCTTGAAGCTGGCTGTC TTCATGTGGCTGATGACCTATGTTGGTGCTGTTTTTAATGGAATTACCCTTCTGATTCTCGCCGAGCTGC TTGTCTTCAGCGTCCCAATTGTCTATGAGAAGTATAAGACACAGATTGACCACTATGTTGGGATTGCCCG GGATCAGACCAAGTCAATTGTTGAAAAGATCCAAGCAAAGCTTCCTGGAATCGCCAAAAAAAAGGCAGAA TAAGCACGTGAAGCAAGAAGTGCATCAGTTACTAAAACACCATTTAATAGTTATAACATCATCACTTGTA CTATGAAGGAACTTGCTCAGTGTCAGCTAGAGTTTGGTTCCAAGTTGTTTGTTCATTTTAATTTGGTGTT CTCTCCCCTCCTCTCCCTTTACCCTCAGTACCAAGCACAAGATGTGTTAGACTGAAAAAAGAACTGATCT CTTAGAACCGTAAAGAATAAAGATTGAATCAGATTACTAGAATTAATCAGACCATAGTGGGGTGGAGCTT TACATGTACCATAAAAGGAGAAAAAGAGAAGTGTCTCTAGGGTCCTCTCTCCAGTTCCAAGGACTCGTAT ACAGCTCCCATTTATAATTTTGCCCTTATTTTTAAGTTAGGAAATAATGATTAACTTATGAATAAATAGT ATGGGGCAAGAGTGGGATTTCTTCCGTTGGGTTTGTATGGCCCTCCAGTCCCACCTTCCTGCCTTACACA TAAGCAGCTACTCTTACTGCTCCTCTTCTTCCTTAATGAAAGAACTCTCAGCTCTGAATAACTTACAGAA CTCCCAGAATGCCACCTGAGAAATGGGGGTTGGGGGGACAGTGGGTGGAGCAGTCAGCAGGAGGGCAGCC ACCACCCTCTCTGCGGCTGCTCCTAGGGAAGGAAGGGCTCTGGGGCTGGGAGGGCCGTGTACAGCCCTTG GTCTGGGATCCATGTGTTGGTTGAGAAATCATTCCAGAGTGTGCTTTCCCCTCTCCCTGCCCACCTTACC TCACGTTTAATAAATATGGTTGTACTTTTCTTATTATGAAATAAATGTCTGTAACTGCTGTACACTGCTA TAAACTTGTTAAAGAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6839323 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6839323 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12146 same embryo
	EMAGE:12144 same embryo
	EMAGE:12143 same embryo
	EMAGE:12145 same embryo
	EMAGE:12141 same embryo
	EurExpress:euxassay_008415 same experiment
	MGI:4827829 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source