EMAGE mouse gene expression spatial database

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EMAGE:12205

Sdcbp syndecan binding protein ( MGI:1337026)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008333. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008333_01	euxassay_008333_02	euxassay_008333_03	euxassay_008333_04

euxassay_008333_05	euxassay_008333_06	euxassay_008333_07	euxassay_008333_08	euxassay_008333_09

euxassay_008333_10	euxassay_008333_11	euxassay_008333_12	euxassay_008333_13	euxassay_008333_14

euxassay_008333_15	euxassay_008333_16	euxassay_008333_17	euxassay_008333_18	euxassay_008333_19

euxassay_008333_20	euxassay_008333_21	euxassay_008333_22	euxassay_008333_23	euxassay_008333_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12205_wholemount_strong.wlz

12205_wholemount_moderate.wlz

12205_wholemount_weak.wlz

12205_wholemount_possible.wlz

12205_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12205_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

naris

strong

regional

strong expression: see section 16 19

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 10 11 12 13 14 17 20 21

nasal cavity respiratory epithelium

strong

regional

strong expression: see section 14 15 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7114
Entity Detected:	Sdcbp, syndecan binding protein ( MGI:1337026)
Sequence:	sense strand is shown >T7114 TGGAGCTGGAGCGGGCAGCAGGAGAGCGCGGTCCTCGGGGCAGAAAATATCTGCAGAAATGTCTCTTTAT CCATCTCTTGAAGATTTGAAGGTAGACAAAGTAATTCAGGCTCAAACTGCTTATTCTGCCAATCCCGCCA GCCAAGCATTTGTTCTGGTGGATGCTTCTGCTGCTCTTCCTCCAGATGGAAATCTGTATCCTAAACTGTA TCCAGAGCTCTCTCAATACATGGGACTGTCTTTAAATGAAGCTGAAATATGTGAGAGCATGCCCATGGTC TCTGGAGCACCAGCACAAGGGCAGTTGGTAGCAAGACCATCCAGTGTGAACTATATGGTAGCACCTGTGA CAGGTAACGATGCTGGAATTCGGAGAGCAGAAATTAAGCAAGGGATTCGTGAAGTTATTTTGTGTAAGGA TCAAGATGGAAAAATTGGGCTCAGACTTAAGTCAATAGATAATGGTATATTTGTTCAACTCGTCCAGGCT AATTCCCCAGCCTCCTTGGTTGGACTGAGATTTGGGGACCAGGTGCTCCAGATCAACGGTGAAAACTGTG CGGGCTGGAGCTCTGATAAGGCACACAAGGTGCTCAAGCAGGCGTTTGGAGAGAAGATCACCATGACGAT CCGTGACAGGCCCTTTGAACGGACAGTTACTATGCATAAGGACAGCAGTGGACATGTTGGCTTTATCTTT AAAAGTGGAAAAATCACATCCATAGTAAAAGATAGTTCTGCTGCCAGAAACGGCCTCCTCACTGACCACC ACATCTGTGAGATCAACGGACAGAACGTCATTGGCTTGAAGGATGCTCAGATTGCAGATATACTGTCAAC AGCTGGGACTGTGGTTACCATCACAATCATGCCTACTTTTATCTTCGAACACATTATTAAACGGATGGCT CCCAGCATTATGAAAAGCCTGATGGATCACACCATTCCTGAAGTTTAAACCCTTGTACAATGCATCACGG CTGAATGTTTCCAGTTTTCCTCTTCGGCAACTTGTCTATTTCGCACATGAAGCTTTCCCGGAGCCAGGGA GAATCTGCTGCATGGAGACCTTTCTATCTTCTCTCATAGCTGGGAACCCGCTCTATCTGATACTGGCTTC AGATTTTAAGATGGGTGTAGCCTTAGCCTGGTTTTACAGATGTGAGACTCCCAAAAGATTTACTGACTTT CCTAGACTGGGTTCTTTACTGGGAACCTGATGTTTTTATATAAGCCATTGTGATTACGATGACTGGTATA GGCTTAGCTGTGTCTGAGACCTGGTCAGCTTTCTTCTAGAACAAAGCACAGTGCTAGTCATGTGCTTCAA TTCTGTGGCATTCTTGGACTTTAGTACATCAGCATAGTACATTTAGAATGATTGCCTTTGATAGTTATTT TTTTAAGTTTTCTCTGTGTGAGTGTGTAAAATTCCAATTAATCAACACTGTTTTCATTCCACAAAAGCAT TACACAGTTGTGTGTAGGTTGCAAGAGACTGTAATGATTGTCACTAATCTTAACTACCTTCACTTAATAT GCTTCAATTGTCGCCTTAACTACAGTAAGCATTTAGAATAAAAGCCAAAATAGAATTATTGCTGCCTTCT GAAACTTGAGATGTTAGCTCTCTATTAAGCTGTTCTGTGCTAGCCCTGAGCAGCTCACTGGTAAAGCAGA GCTGCCTACTTGTATCAAGATTTTCTAGTCACTGCATAAAGGAAACGCCTAAGAGTTGCCGTATTGCTTT CTGAGAAACAAATTGCTATATATTTCATGTAAAAGTTGTTATACTAAATAGAATAAATTTTGGAGTCTCT TTTTCACTCTAGTGGGTAATATCTAATTTGGCATTTGAGTCCTAACATTATGTAACTTTAGCTAGGTTGG GGTCGTTTTAGAATGTTTCCCTAGTCATCTTAATTTGTTCCATTTTCTGACTCTGTCCTATAAACAAAAA TGGCAATGACACTTTATGATTTTTTCTTTCTTAGCTTGTGCCTATCCTATTATTTTATGTGATATTATTA AATGTATAAAAATGTGAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4017442 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4017442 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12208 same embryo
	EMAGE:12204 same embryo
	EMAGE:12203 same embryo
	EMAGE:12207 same embryo
	EMAGE:12206 same embryo
	EurExpress:euxassay_008333 same experiment
	MGI:4827921 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source