EMAGE mouse gene expression spatial database

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EMAGE:12235

Kcnq3 potassium voltage-gated channel, subfamily Q, member 3 ( MGI:1336181)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008387. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008387_01	euxassay_008387_02	euxassay_008387_03	euxassay_008387_04

euxassay_008387_05	euxassay_008387_06	euxassay_008387_07	euxassay_008387_08	euxassay_008387_09

euxassay_008387_10	euxassay_008387_11	euxassay_008387_12	euxassay_008387_13	euxassay_008387_14

euxassay_008387_15	euxassay_008387_16	euxassay_008387_17	euxassay_008387_18	euxassay_008387_19

euxassay_008387_20	euxassay_008387_21	euxassay_008387_22	euxassay_008387_23	euxassay_008387_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12235_wholemount_strong.wlz

12235_wholemount_moderate.wlz

12235_wholemount_weak.wlz

12235_wholemount_possible.wlz

12235_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12235_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

moderate

regional

moderate expression: see section 22 23 weak expression: see section 03 04 05 19 20 21

telencephalon mantle layer

strong

regional

strong expression: see section 03 04 05 06 07 20 21 22 23 24 moderate expression: see section 01 02

telencephalon marginal layer

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 12 14 17 18 19 20 21 22 23 moderate expression: see section 15

ventral grey horn

weak

regional

weak expression: see section 10 11 14 15 16

midgut

moderate

regional

moderate expression: see section 09 10 weak expression: see section 11 13 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T35254
Entity Detected:	Kcnq3, potassium voltage-gated channel, subfamily Q, member 3 ( MGI:1336181)
Sequence:	sense strand is shown >T35254 AATGAACCATATGTAGCCAGGGCAGCCACATCAGAAACTGAAGACCAAAGCATGATGGGGAAGTTTGTAA AAGTTGAAAGACAGGTTCATGACATGGGGAAGAAACTGGACTTCCTCGTGGACATGCATATGCAGCATAT GGAACGCTTACAGGTACATGTCACTGAGTACTACCCAACTAAGGGGGCCTCCTCCCCAGCCGAAGGGGAG AAGAAAGAAGACAACAGGTACTCTGATTTGAAAACCATCATCTGCAACTACTCAGAGACAGGGCCCCCTG ACCCTCCTTACAGCTTCCACCAGGTGCCCATCGACAGAGTTGGCCCTTACGGGTTTTTTGCACATGATCC TGTGAAACTGACCCGAGGGGGACCCAGTTCTACAAAGGCTCAAGCTAACCTTCCCTCCTCGGGAAGTACA TATGCAGAGAGGCCCACAGTCCTGCCCATCTTGACTCTTCTGGACTCATGTGTGAGCTACCACTCCCAGA CAGAACTGCAAGGTCCCTATTCGGACCACATCTCGCCCCGCCAGAGACGCAGCATCACTAGGGACAGTGA TACACCTCTGTCCCTCATGTCCGTCAATCACGAGGAACTGGAGCGGTCTCCAAGTGGCTTCAGCATCTCA CAAGACAGAGA
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 92974. Forward Primer - name:092974_F_cDNA_Kcnq3, sequence:AATGAACCATATGTAGCCAGGG; Reverse Primer - name:092974_N_SP6_cDNA_Kcnq3, sequence:TCTCTGTCTTGTGAGATGCTGA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12233 same embryo
	EMAGE:12236 same embryo
	EMAGE:12232 same embryo
	EMAGE:12234 same embryo
	EurExpress:euxassay_008387 same experiment
	MGI:4825729 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source