EMAGE mouse gene expression spatial database

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EMAGE:12487

Adamts19 a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 19 ( MGI:2442875)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011355. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011355_01	euxassay_011355_02	euxassay_011355_03	euxassay_011355_04

euxassay_011355_05	euxassay_011355_06	euxassay_011355_07	euxassay_011355_08	euxassay_011355_09

euxassay_011355_10	euxassay_011355_11	euxassay_011355_12	euxassay_011355_13	euxassay_011355_14

euxassay_011355_15	euxassay_011355_16	euxassay_011355_17	euxassay_011355_18	euxassay_011355_19

euxassay_011355_20	euxassay_011355_21	euxassay_011355_22	euxassay_011355_23	euxassay_011355_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12487_wholemount_strong.wlz

12487_wholemount_moderate.wlz

12487_wholemount_weak.wlz

12487_wholemount_possible.wlz

12487_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12487_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

weak

regional

weak expression: see section 10 11 19 20 21

vibrissa

weak

regional

weak expression: see section 07 08 09 23 24

cerebral cortex ventricular layer

moderate

regional

moderate expression: see section 09 10 11 12 14 15 16 17 weak expression: see section 05 06 07 08 18 19 20

cochlea

strong

regional

strong expression: see section 07 08 09 10 18 19 20 21

utricle

strong

regional

strong expression: see section 02 03 05 06 07 08 09 19 20 21 22 24

perioptic mesenchyme

weak

regional

weak expression: see section 04 05 24

naris

weak

regional

weak expression: see section 13 14 15 16 17 18

viscerocranium

weak

regional

Expression in the turbinate bone.

heart valve

strong

regional

strong expression: see section 14 15 16 moderate expression: see section 12 13

stomach mesentery

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13

thyroid cartilage

weak

regional

weak expression: see section 13 14 16

cranium

weak

regional

weak expression: see section 03 04 05 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T37546
Entity Detected:	Adamts19, a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 19 ( MGI:2442875)
Sequence:	sense strand is shown >T37546 GTCCAGAGTAGACAAGTGGCCTGTACCCAGCAGCTGGAAAATGGAACCCTGATTAGAGCCTGGGAGAGGG ACTGCCTAGGGCCAAAGCCCGCAACTGTGCAACGCTGTGAGGGTCAGGACTGCATGACGGTGTGGGAGGC CGGAGTGTGGTCGGAGTGCTCAGTGAAGTGTGGCAAAGGTGTGCGGCATCGCACCGTGAGGTGCACTAAC CCGAGAAAAAAGTGCGTCCTCTCCACCAGACCCAGGGAGGCCGAGGACTGTGAGGATTACTCCAAATGCT ACGTGTGGCGAGTAGGTGACTGGTCTAAGTGCTCCATTACCTGTGGCAAAGGCATGCAGTCTCGTGTTAT CCAGTGCATGCATAAGATCACAGGAAGACACGGAAATGAATGCTTTTCTTCGGAGAAACCTGCAGCCTAC AGGCCATGTCACCTTCAGCCCTGTAATGAGAAAATTAATGTAAATACAATAACATCACCAAGACTGGCTG CCCTGACCTTCAAGTGCCTGGGAGACCAGTGGCCAGTGTACTGCAGAGTGATCCGGGAGAAGAATCTTTG CCAGGACATGCGGTGGTACCAACGATGCTGTGAGACTTGCAGGGACTTCTATGCCCAAAAGCTACAGCAG AAGAGTTGACCTCTGACAGGCT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 93664. Forward Primer - name:093664_F_cDNA_Adamts19, sequence:GTCCAGAGTAGACAAGTGGCCT; Reverse Primer - name:093664_N_SP6_cDNA_Adamts19, sequence:AGCCTGTCAGAGGTCAACTCTTC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12488 same embryo
	EMAGE:12489 same embryo
	EMAGE:12490 same embryo
	EMAGE:12491 same embryo
	EurExpress:euxassay_011355 same experiment
	MGI:4822987 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source