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EMAGE:12536

4931429I11Rik RIKEN cDNA 4931429I11 gene ( MGI:1918239)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:12536 EMAGE:12536 EMAGE:12536 EMAGE:12536 EMAGE:12536
"Pseudo-wholemount" of euxassay_011253. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_011253_01 euxassay_011253_02 euxassay_011253_03 euxassay_011253_04
EMAGE:12536 EMAGE:12536 EMAGE:12536 EMAGE:12536 EMAGE:12536
euxassay_011253_05 euxassay_011253_06 euxassay_011253_07 euxassay_011253_08 euxassay_011253_09
EMAGE:12536 EMAGE:12536 EMAGE:12536 EMAGE:12536 EMAGE:12536
euxassay_011253_10 euxassay_011253_11 euxassay_011253_12 euxassay_011253_13 euxassay_011253_14
EMAGE:12536 EMAGE:12536 EMAGE:12536 EMAGE:12536 EMAGE:12536
euxassay_011253_15 euxassay_011253_16 euxassay_011253_17 euxassay_011253_18 euxassay_011253_19
EMAGE:12536 EMAGE:12536 EMAGE:12536 EMAGE:12536 EMAGE:12536
euxassay_011253_20 euxassay_011253_21 euxassay_011253_22 euxassay_011253_23 euxassay_011253_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:12536Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
12536_wholemount_strong.wlz
12536_wholemount_moderate.wlz
12536_wholemount_weak.wlz
12536_wholemount_possible.wlz
12536_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:12536_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
spinal cord floor plate
strong strong
regionalstrong expression: see section 11 weak expression: see section 12 13
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T37397
Entity Detected:4931429I11Rik, RIKEN cDNA 4931429I11 gene ( MGI:1918239)
Sequence:sense strand is shown

>T37397
CAGAATAACCCTTTCCAGCAACCACAGAACCAAAGGCAGACTGCGGATGCCTCAGCAAAGCATGAGCTGG
CTGCACAAACGAACGCTTCTAACCCCAATTTACAGGACGCCAGAACGCTCACCCACAATCCCAAAGTCAC
CTCCGACTCTTTCGTTTCACCTAAGCAGGCTTTAGACAGAGCTTTATATAAAAACTCCATCTCAGGACTG
AACGCTAATAAAAGACGAGGACACAGACGTGAGGAAGAGAGAATTCTGTATCAGCCGCAGCCCATCTACG
TGTTTTCTGATACACATCTGCAGGACTTTAATGAACTTCCTCACAGACACGAGTCCCCGAGCCAGAGAGC
CCCTCAGTCCGATCACCACATGAACACACACAGGTCAACGAAGACAAAGAAGCCAGCCAAACAACCGCAG
GCAGAGACGAAATACAAGAACATAGAGATGCTCTGGAAATTCCACTCTTCCTCAGACATGGAACCCGCGA
GTGCCTCGCCAGACTCCCGGCTTGCCCAGATCATGGAACAGCACCAGCAAGCCTTGATGCAGCTGGCAGA
GGTGCAGCCCAGCGAGGGCTCTCTGGCCAGCATCATACTCCCACCTATCC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 81808. Forward Primer - name:081808_F_cDNA_4931429I11Rik, sequence:CAGAATAACCCTTTCCAGCAAC; Reverse Primer - name:081808_N_SP6_cDNA_4931429I11Rik, sequence:GGATAGGTGGGAGTATGATGC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:12537 same embryo
 EurExpress:euxassay_011253 same experiment
 MGI:4822748 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS