EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:12574

Pex10 peroxisomal biogenesis factor 10 ( MGI:2684988)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_013750. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_013750_01	euxassay_013750_02	euxassay_013750_03	euxassay_013750_04

euxassay_013750_05	euxassay_013750_06	euxassay_013750_07	euxassay_013750_08	euxassay_013750_09

euxassay_013750_10	euxassay_013750_11	euxassay_013750_12	euxassay_013750_13	euxassay_013750_14

euxassay_013750_15	euxassay_013750_16	euxassay_013750_17	euxassay_013750_18	euxassay_013750_19

euxassay_013750_20	euxassay_013750_21	euxassay_013750_22	euxassay_013750_23	euxassay_013750_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12574_wholemount_strong.wlz

12574_wholemount_moderate.wlz

12574_wholemount_weak.wlz

12574_wholemount_possible.wlz

12574_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12574_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata alar plate mantle layer

strong

regional

strong expression: see section 09 10

medulla oblongata basal plate mantle layer

strong

regional

strong expression: see section 09 10

pons mantle layer

strong

regional

strong expression: see section 09 10

midbrain mantle layer

strong

regional

strong expression: see section 09 10 11

dorsal grey horn

strong

regional

strong expression: see section 11

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T37821
Entity Detected:	Pex10, peroxisomal biogenesis factor 10 ( MGI:2684988)
Sequence:	sense strand is shown >T37821 CCTACCTGCTAGACAAGGCACTGCTGCCCCTGGAGCAGGAGCTGCAAGCCGATGGCGATGCTCCCCGGGC TTCACAGGGCAGTCTGCTACCTGGAGGACGCAGCCGATCAGGGGCACGGCGCTGGGTGCGTCACCACGCA GCCACCCTGACTGAACAACAGAGGAAGGCCCTCCAGCGGGCAGTCTTCATCCTGAGACAGGGCTTCGCCT GCCTCCATCGGCTCCACGTGGCTTGGTTTTATATTCATGGTACCTTCTACCACCTGGCCAAGAGACTAGC AGGGATCACGTATCTCCGCACTCGTCGCCTGCCTGGAGAGGACCAGAAGGCTCGCACTAGCTACGGACTG CTGGGGCTGATCTCCCTCTTGCATCTGGTGCTGTCCATGGGGCTGCGGTTGTACAGCTTCAGGCAGAAGC AGCGAGCCAGGAAGGAGTGGCGGCTGCACCGCAATCTATCCCACCGCAGGAGTTCCCTTGAAGACAGAGC CGTGTGCAGAACCCCACTCTGCACCCTATGCTTAGAGGAACGAAGACACTCCACGGCCACACCTTGTGGC CATCTCTTCTGCTGGGAGTGCATC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 78443. Forward Primer - name:078443_F_cDNA_LOC230983, sequence:CCTACCTGCTAGACAAGGCACT; Reverse Primer - name:078443_N_SP6_cDNA_LOC230983, sequence:GATGCACTCCCAGCAGAAGAG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12573 same embryo
	EMAGE:12576 same embryo
	EMAGE:12575 same embryo
	EurExpress:euxassay_013750 same experiment
	MGI:4827158 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source