EMAGE mouse gene expression spatial database

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EMAGE:12576

Plk1s1 polo-like kinase 1 substrate 1 ( MGI:2684960)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_013723. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_013723_01	euxassay_013723_02	euxassay_013723_03	euxassay_013723_04

euxassay_013723_05	euxassay_013723_06	euxassay_013723_07	euxassay_013723_08	euxassay_013723_09

euxassay_013723_10	euxassay_013723_11	euxassay_013723_12	euxassay_013723_13	euxassay_013723_14

euxassay_013723_15	euxassay_013723_16	euxassay_013723_17	euxassay_013723_18	euxassay_013723_19

euxassay_013723_20	euxassay_013723_21	euxassay_013723_22	euxassay_013723_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12576_wholemount_strong.wlz

12576_wholemount_moderate.wlz

12576_wholemount_weak.wlz

12576_wholemount_possible.wlz

12576_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12576_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thyroid gland

strong

regional

strong expression: see section 12 16 17

diencephalon lateral wall mantle layer

strong

regional

strong expression: see section 09 10 11 14 15 moderate expression: see section 06 07 08 16 17 18 19

diencephalon lateral wall ventricular layer

strong

regional

strong expression: see section 12 13 14

telencephalon mantle layer

strong

regional

strong expression: see section 05 moderate expression: see section 06 09 10 11 12 13 14 15

telencephalon ventricular layer

moderate

regional

moderate expression: see section 13

midbrain mantle layer

strong

regional

strong expression: see section 06 07 08 09 17 18 19 20 moderate expression: see section 10 11 12 14 15 16

midbrain marginal layer

moderate

regional

moderate expression: see section 10 11 12 14 15 16

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 08 09 10 16 17 18 moderate expression: see section 11 12 13 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T37817
Entity Detected:	Plk1s1, polo-like kinase 1 substrate 1 ( MGI:2684960)
Sequence:	sense strand is shown >T37817 TCAATCTCTGACCCACATTCACACCAACAGACAGCCCAGAGCAGCTGTGTGACAGACAGCTGTGTAGTGC AGACTAATGGTGACACACAGTGCTTAAATAAGTCTGACAAAATACATGGAAAGACATCTCTTCAGACTGG TGAGAAGGCGCCAGTCACAAGCTATGTATTGTCTGCAGAAGAACAAACTCATTGCTTGGAGATAGGAAGC AGCACACAGCACAGCAAGAGTAATTTATCTGAAGGCAGAAAGTCTGCTGAACTCCATTCCTCGTTACAGG AAAGATTAAGTCCAGAGAACAGCATCACTGATTTAAAGTGTGACAGTTCCAGCAGATCAGAGGGATCAGA CAGAGAAATACTGACACAAGAACATATTGAAGTCAGGGAAGAAAGAGCCGGCCCGCTGGTCCCCATGATG GCAGCTTCAGAACACTGTACATCTGCAAAGAAGTGGGCTGGAGAGAAGCATTCTGCTTGGGAAGCTTCCT CAGATGATCTTGACCACGGGGACTCAAAGTCACAGAAGGCCGTCCTGAAACATGAGGAAGAGCAGGAGGA GGGAAGCTCATGCAGCAGCAGCGACCTCACAGTTTCTGTCAGTGAAGATGATCTGATTCTAGAGAGTCTG GCCGCACTGTCGAATCCAGGAGCCAAGAT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 79768. Forward Primer - name:079768_F_cDNA_LOC228730, sequence:TCAATCTCTGACCCACATTCAC; Reverse Primer - name:079768_N_SP6_cDNA_LOC228730, sequence:ATCTTGGCTCCTGGATTCGAC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12573 same embryo
	EMAGE:12575 same embryo
	EMAGE:12574 same embryo
	EurExpress:euxassay_013723 same experiment
	MGI:4827273 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source