EMAGE mouse gene expression spatial database

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EMAGE:12692

Kcng3 potassium voltage-gated channel, subfamily G, member 3 ( MGI:2663923)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011450. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011450_01	euxassay_011450_02	euxassay_011450_03	euxassay_011450_04

euxassay_011450_05	euxassay_011450_06	euxassay_011450_07	euxassay_011450_08	euxassay_011450_09

euxassay_011450_10	euxassay_011450_11	euxassay_011450_12	euxassay_011450_13	euxassay_011450_14

euxassay_011450_15	euxassay_011450_16	euxassay_011450_17	euxassay_011450_18	euxassay_011450_19

euxassay_011450_20	euxassay_011450_21	euxassay_011450_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12692_wholemount_strong.wlz

12692_wholemount_moderate.wlz

12692_wholemount_weak.wlz

12692_wholemount_possible.wlz

12692_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12692_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hypothalamus mantle layer

weak

regional

weak expression: see section 15

diencephalon lateral wall mantle layer

weak

regional

weak expression: see section 12 13 14 15

midbrain mantle layer

weak

regional

weak expression: see section 12 13

lung

moderate

spotted

moderate expression: see section 08 09 10 11 15 16 17 18 19 20 21 22 weak expression: see section 06 07

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T37764
Entity Detected:	Kcng3, potassium voltage-gated channel, subfamily G, member 3 ( MGI:2663923)
Sequence:	sense strand is shown >T37764 CATAGCTTTGTGCAGTGCTACCACGAGCTCAAGTTTAGATCGGCTAGATATAGTAGGAGCCTCTCAGCTG AGTTCCTGAATTAACGTGTTGCAAACCAGTCCTTGTGCACACTTCATCTGGTCGAGTTGACACTAGTTCT TCTTCGCGTGTTTCTCCCTGGGTGAGCACTGCAATGGTGTTGTCCTCCTGTTGGAAGGGTGACATGATTC TTCCCAACTCAAGGGTTAAAGCGGTTTACTTACGGAGTAGGCAGGACCGGGACCGGGACACACAGGACAG TAGCACCAGAGGAGATGCTAGGACCTGGTTTTATTCCCTCTCGTCTCCCTCCACCTTGCCTCGCACAGTT ACGATGTTGTAGGCTGTTCTTAAGCACATTATTTGAACACTTTAAAGCAGAAAGACACTATTTTCCAAAT GCTTTTCCATCTTAACGAATTCAGAAGAAGCTTGGAACTTAACAGTGTTATTTTTGAGACTAACATTTTC GTTTCTAAATGTTTTATAATTTCTCATATCAATGTCAGAAGTATCCTGGAAACATATGCCACATGTGGGA ACTGTTTAACAAATACTTTAAAAAGTTGGCCCAAATTTAAACTGTATTGCGGAACTAGAGGCAAGCAAAA ATATTATTTGAAAAGCTTTGCACCCTTCTCTAGCCTACCCTTGGCTTAATTTTTACACCTGTCACTTCTC TTATCTTACAACTTTATGGACCCTGAAGTGTGTTTTCTCTTTCCAGTTAGGCCCCCACTTTACTGAAATA ACTTCCAAAGAGTACATGATATCACGGAGAGACCTTGTGTTGTTTAGCGCTCTGT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 93024. Forward Primer - name:093024_F_cDNA_Kcng3, sequence:CATAGCTTTGTGCAGTGCTACC; Reverse Primer - name:093024_N_SP6_cDNA_Kcng3, sequence:ACAGAGCGCTAAACAACACAAG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12696 same embryo
	EMAGE:12694 same embryo
	EMAGE:12693 same embryo
	EMAGE:12695 same embryo
	EMAGE:12691 same embryo
	EurExpress:euxassay_011450 same experiment
	MGI:4825709 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source