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EMAGE:12882

Baz2b bromodomain adjacent to zinc finger domain, 2B ( MGI:2442782)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:12882 EMAGE:12882 EMAGE:12882 EMAGE:12882 EMAGE:12882
"Pseudo-wholemount" of euxassay_011654. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_011654_01 euxassay_011654_02 euxassay_011654_03 euxassay_011654_04
EMAGE:12882 EMAGE:12882 EMAGE:12882 EMAGE:12882 EMAGE:12882
euxassay_011654_05 euxassay_011654_06 euxassay_011654_07 euxassay_011654_08 euxassay_011654_09
EMAGE:12882 EMAGE:12882 EMAGE:12882 EMAGE:12882 EMAGE:12882
euxassay_011654_10 euxassay_011654_11 euxassay_011654_12 euxassay_011654_13 euxassay_011654_14
EMAGE:12882 EMAGE:12882 EMAGE:12882 EMAGE:12882 EMAGE:12882
euxassay_011654_15 euxassay_011654_16 euxassay_011654_17 euxassay_011654_18 euxassay_011654_19
EMAGE:12882 EMAGE:12882 EMAGE:12882 EMAGE:12882
euxassay_011654_20 euxassay_011654_21 euxassay_011654_22 euxassay_011654_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:12882Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
12882_wholemount_strong.wlz
12882_wholemount_moderate.wlz
12882_wholemount_weak.wlz
12882_wholemount_possible.wlz
12882_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:12882_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
nasal cavity olfactory epithelium
weak weak
regionalweak expression: see section 10 11 12 13 16 17 18
vomeronasal organ
weak weak
regionalweak expression: see section 16
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T31714
Entity Detected:Baz2b, bromodomain adjacent to zinc finger domain, 2B ( MGI:2442782)
Sequence:sense strand is shown

>T31714
TCCACAAGCAGCTCCCTCAAGAGAGGAAGCAAAGAGCTCAAAAAGAGGAAAATGGAGGAAACCACTTCCC
TCAACCTGTCAAAAGCAGAAAGCACCACCTCCATTAAGAAACCCAAAAAAGATGAGTCCAGGGACCTGGC
TCTCTGCAGTATGATCCTGACTGAAATGGAGACACATGAGGACTCCTGGCCTTTCCTGCTTCCTGTGAAC
TTGAAACTCGTACCCGGCTATAAGAAGGTTATCAAGAAACCTATGGATTTTTCTACAATTAGAGAAAAAC
TAAATAATGGACAGTATCCCAACTTTGAGACCTTTGCTCTAGACGTCAGGCTTGTTTTTGACAACTGTGA
AACATTTAATGAAGATGACTCGGACATAGGCAGAGCTGGC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:5357055), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 57817. Forward Primer - name:057817_F_IRAV87_g11_BC053917, sequence:TCCACAAGCAGCTCCCTC; Reverse Primer - name:057817_R_SP6_IRAV87_g11_BC053917, sequence:GGCCAGCTCTGCCTATGT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:12880 same embryo
 EMAGE:12879 same embryo
 EMAGE:12878 same embryo
 EMAGE:12881 same embryo
 EMAGE:12877 same embryo
 EurExpress:euxassay_011654 same experiment
 MGI:4823397 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS