EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:13021

Sh3tc1 SH3 domain and tetratricopeptide repeats 1 ( MGI:2678949)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_013707. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_013707_01	euxassay_013707_02	euxassay_013707_03	euxassay_013707_04

euxassay_013707_05	euxassay_013707_06	euxassay_013707_07	euxassay_013707_08	euxassay_013707_09

euxassay_013707_10	euxassay_013707_11	euxassay_013707_12	euxassay_013707_13	euxassay_013707_14

euxassay_013707_15	euxassay_013707_16	euxassay_013707_17	euxassay_013707_18	euxassay_013707_19

euxassay_013707_20	euxassay_013707_21	euxassay_013707_22	euxassay_013707_23	euxassay_013707_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13021_wholemount_strong.wlz

13021_wholemount_moderate.wlz

13021_wholemount_weak.wlz

13021_wholemount_possible.wlz

13021_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13021_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

mesenchyme

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

diencephalon meninges

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18

telencephalon meninges

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

hindbrain meninges

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

midbrain meninges

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18

spinal cord meninges

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8634
Entity Detected:	Sh3tc1, SH3 domain and tetratricopeptide repeats 1 ( MGI:2678949)
Sequence:	sense strand is shown >T8634 GGTCTGCGGAGGCTGAGGCAACAGTGAGAGGTGATGTGGCTCCTCCCCGCATCGTGGTCGGGCTGGCTGC TGAACCCCCTCCCAGTCAAATGACAGTGTATCCAACAGGAACTGTCAGCCAGGCTGCTGTCTATCCACAG TGACCAGGACCGGATCGTGGTGACATTTAAGACTTTTGAAGAAATCTGGAAGTTTTCTACCTATCATGCT CTTGGCTTCACACACCACTGCCTGGAGAACCTGCTGGTAGACCAGACCTTCTGGCTGCTTTCCCCCAGTG AGGAGGAGGAGACTGCCATCCTCATGTATGTAAACAAGGATGCCTTGAAGCAGACACACAAGAACCTCCT CACACAGGAAGGGTCCATCTTTGTTCTGTGTCCTGACCACCGAGTAAGAAGGACGATAGGACCCAAAGGT GCAGGGAAGGGTCCCCAGACCCTCAGACGGGCTTCGGGAGTCCCTCTGGGAGTGGCAATCCTGGAAACAG ACTCCTCATCACCCACACCCAACATGTCCTCCAATGTGGAAGCAGCAGAGGCCGAACCAGAGCCCTTGAC TCCATTTCACCAGTGGACTCTCAGGGTCCCATGGGATCCCATCGATGAATCCATGAGTGGACCTGGGACA CCTGATGCCCAGCTCATGAGGTTTGGCCTGGCCTCTGCGATAGCTGACTTCCAGGGCTCAGGCCCCGAAG AGATGTCCTTCAGCGTTGGTGATGTCATCGAGATCCTCGGTGCCCAGGTGCCTGGCCTGCCCTGGTGTGT GGGCCGGCACATGGCCTCTGGCCAGGTGGGCTTTGTGAGGACTGGCCTCGTCAGCATGCAGGGCCCGGCA TCTGACCTGGAAAGTGCCATTTTCCTCAGTGAGGAAGAAAGGTCATTCTTCAGAAGTGAGGGCCACTTCT CAGACGAAGATGCCCGGCGGCTCCTGAGCAGGACATCTGGCACAGATGTGTGCACCACATGCAGCCTGGA CTGGTTAGAAGAAGCTGAGGGCGAACAGCTGGAGCAACGAGAAATGTCTCTGCCTCACCTGAACCCAGAA CCACATGGGACTCTGCAGATGGTAAAGAACATTTTAGAAAAGTGCAAGGCCTGCCCTGACCACCCCGAAG CCCCGGTGTCCTGGAGCCTTGGAGGGGTGTCCAGAAGAGCGAGTTCACTGGACCTTGAGGAGCCCCGCTT CTGCCTGGATCCTGAGGATGACTGGACAGACCCCGAGCCTCTGGACTCACTGCTGCAGGTCCTGAATGCC CCTGGTTATGGGGCCCACTTCCGGAGCCTGTACGATATCTCACTTCCCTGGCTGAGCACCGCACTCTATG GCTTTGATGATGAAGAAGAGCTGGCTGAACGATTGGCACAGGCCCGAGGAGTTGCCAAGAAAGTCGACTT GAGCATGGCCCTGGCAAGGCTCTGCCTCCTGCTGGGGCGTCTGTGTGCCCGAAAGCTCAAACTATCCCAG GCCCGGGTGTACTTTGAAGAGGCACTGGGAGCTCTGGAGGGGAGTTTTGGGGACCTCTCCCTGGTGGCGG CAGTGTATAGCAGCCTGACTACTGTGTACCTAAAGCAGAAGAACGCAGAGAAGTGTGTGCAGGTGGCACC CAAAGCAGCGGCACTGCTCCTGGGGACACCCGGCCACTCCTGCAACGCAGATGCGGAGCTGCTGAAGTAT GCGCTGCGCAGGGTCATCTGTGGCCTCAGCCCACAGGCCGAGGCCCGGGCCTGCTTCCTGCTGGCCAGGC ACTACACCCACCTCAAACAGCCTGAGGAGGCCCTGCCATACCTGGAGAGACTGCTTCGCCTCAACAGGGA TGCAGGGAGCCCACAGGCCTCATGGCCTGAAGACTGTTACCTGCTTCTGGCTGACATTTATGGCCGGAAA TGCCTGCCCCATCTGGCGTTGAGCTGCCTCAGAGTGTCCTCACTGTGGACAAGGTGCTCTCTGCCTGGCT CCTTGAGGAGTGTGGACCTGGTACTCCAGAATGTTCCTGGGCCAAACAGCCAAAGGAGGGCAGGTCATAG CCTTCCCTCCCAGATTGCTTACTACCTCAGACAGGCATTGGCCTCTCTGGCTCCTGGCACAGGACAGGCA CTGCGTGGGCCGCTCTATGCCAGCCTGGCTCAACTGTACAGTCACCACCAGCAGTACAGCCAGGCCATTG CCTTCATGTCTCAGGCTGCAGAAGCTGACACTGCAGCTGGGGTCCACCCTGTCGTGGACCGCTTGGTGGC CCTCGCCTGGTTGCACATGCTTTGTGGGAACAGCTTAGTAGCCATGGACATCCTGAAGTGCATCTCAGAT GCAGCCGTGGCCAATAAGGACCAGGAGTGTGTTATGATGAACATGGTGGCCATGGCCCTGAAGAGGATGG GCAGGACCCGACAGGCTGCTGAAGGCTACTTCCGAGCTCTGCACATGGCCTATGCACAGGGCCACCTGCA GAGCCAGGCAGTGGTCCTGGCTAACTTTGGGGCCCTGTGTCTGCAAGCTGGTGCACGCAGCTTGGCCCAG CACTACCTGCGCGAGGCTGTGGGGCTCTTCTCCCAGGTGCCCAGTGGCGTGTGTGGCCGGGACTTCACCC AGGTGCTCCTGTGGTTGGGCCAACTGTGCACACGCCGGGCCCTCCCGCAGCAGGCTAAGTGCTACTACGA GTGGGCCTTTCTGGTGGCCGTGGAGACAGACCATCTGGAGAGTCAGTTACAGGCTGTGCAGAAGCTGTGC CTCTTCTACAGCAAGGTGATGCCCAATGAGGTGCGATGTGTCATCTACCACGAGTTCCAACTGGCACTGG CACGCAAGACCGCCAACAAGGTGCTGGAGGGACAGCTGCTGGAGGCCATCAGCCAGCTCTACCTGTCGCT GGGCACTGAGCGGGCCTACAAGTCGGCTCTGGATTACACTAAACGGAGCCTGGGGATTTTCATCGACCTG CAGCAGAAGGAGAAGGAGGCACGCGCCTGGCTGCAGGCAGGGAAGATCTACTATATCCTGCGTCAGAATG AGCTGGTGGACTTATACATCCAGGTGGCACAGAATGCAGCCCTGTATACTGGGGACCCCAAGCTGGGCCT GGAACTGTTTGAGGCAGCAGGGGACATTTTCTTCAATGGGACCTGGGAGCGGGAGAAAGCTGTGTCCTTC TACCGGGACCGGGCACTGCCCCTGGCTGTGACTGTAGGAGACCAGGAAGTGGAACTGCGCCTGTGCAACA AGCTGGTGGCCCTTCTGTCCGCGCTGGAGGCACCCCAGGAGGGCCTGGAGTTTGCCCATGAGGCCTTGGC ACTCAGCATCACCCTAGGTGACCGCCTGAATGAGCGAGTGGCCTACCACAGGCTAGCCACCCTGCACCAC CGGCTGGGCCACGGGGAACTGGCTGAGCACTTCTTCCTCAAGGCCCTGTCACTCTGCAGCTCGCCCCTGG AATTCGATGAGGAGACCCTGTACTATGTGAAGGTCTACCTGGTGCTGGGTGACATCATCTTCTATGACCT GAAGGACCCGTTTGATGCAGCTGGGTATTACCAGCTGGCACTGGCAGCAGCCGTGGACCTGGGCCACAAG AAAGCCCAGCTGAAGATCTACACCCGCCTGGCTACCATCTACCACCACTTCCTCGTGGACCGTGAGATGT CCCTCTTCTTCTACCAGAAAGCAAGGACCTTTGCCTCAGAACTGAACCTCCGCAGGACCAACCTGGTGCC CCAGCGCTTCTGTGGCCGGGCACCCTGGCTGGCCCCTGGCCACCCATCCTGACAACCTGAGAAGGGGCAT ATACAAGGGGACTCTTCCTCTTCTTGGGGTGTGGGCAACTTATTATTATTATTATTTTGCTGAGGGTTGG GGGGAACTGAAGGACCAAACAGTAGTAAATATTTTTCAGCAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5720249 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5720249 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13019 same embryo
	EMAGE:13023 same embryo
	EMAGE:13022 same embryo
	EMAGE:13020 same embryo
	EMAGE:13018 same embryo
	EurExpress:euxassay_013707 same experiment
	MGI:4828047 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source