EMAGE mouse gene expression spatial database

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EMAGE:13755

Gtf2h2 general transcription factor II H, polypeptide 2 ( MGI:1345669)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_019537. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_019537_01	euxassay_019537_02	euxassay_019537_03	euxassay_019537_04

euxassay_019537_05	euxassay_019537_06	euxassay_019537_07	euxassay_019537_08	euxassay_019537_09

euxassay_019537_10	euxassay_019537_11	euxassay_019537_12	euxassay_019537_13	euxassay_019537_14

euxassay_019537_15	euxassay_019537_16	euxassay_019537_17	euxassay_019537_18	euxassay_019537_19

euxassay_019537_20	euxassay_019537_21	euxassay_019537_22	euxassay_019537_23	euxassay_019537_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13755_wholemount_strong.wlz

13755_wholemount_moderate.wlz

13755_wholemount_weak.wlz

13755_wholemount_possible.wlz

13755_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13755_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

weak

regional

weak expression: see section 12 13 14 15

submandibular gland primordium

moderate

regional

moderate expression: see section 07 08 09 10 17 18 19

pancreas

weak

regional

weak expression: see section 10 11 12 13 18 19

vibrissa

weak

regional

weak expression: see section 05 06 07 20 21 22

midgut

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 17 18 19 21 weak expression: see section 06 16 20

lower jaw incisor

weak

regional

weak expression: see section 10 11 12 15 16

upper jaw incisor

weak

regional

weak expression: see section 11 12 15 16

liver

moderate

regional

moderate expression: see section 01 02 03 05 06 07 09 10 11 12 13 14 15 17 18 19 21 22 23 weak expression: see section 04 08 20 24

metanephros

weak

regional

weak expression: see section 09 10 11 12 13 19 20 21 22 23

lung

weak

regional

weak expression: see section 04 05 06 07 08 09 10 11 12 13 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T55068
Entity Detected:	Gtf2h2, general transcription factor II H, polypeptide 2 ( MGI:1345669)
Sequence:	sense strand is shown >T55068 AGCTGAGGACCGAGAGTTGAATAAGCATATATATCATTATAAGCACCATGGATGAAGAACCTGAGAGAAC CAAGCGGTGGGAAGGAGGCTATGAGAGAACCTGGGAAATTCTTAAAGAAGATGAAACTGGATCACTTAAA GCTACAATAGAAGATATTCTCTTCAAGGCAAAGAGGAAAAGAGTGTTTGAGCACCATGGACAAGTCCGAC TTGGAATGATGCGCCACCTGTATGTGGTGGTGGATGGATCGAGAACAATGGAAGATCAGGATTTAAAGCC CAATAGACTGACTTGCACTTTAAAGTTGCTGGAATACTTTGTAGAAGAATATTTTGATCAAAACCCTATC AGTCAGATTGGAATAATTGTAACTAAGAGTAAAAGAGCTGAAAAACTGACTGAACTCTCAGGAAACCCAA GGAAACATATAACATCTTTGAAGAAAGCTGTAGATATGACCTGCCATGGAGAACCATCGCTCTATAATTC CTTAAGCATGGCTATGCAGACCCTAAAACACATGCCTGGACATACAAGTAGAGAAGTGCTCATCATCTTC AGTAGCCTCACCACCTGTGATCCATCTAATATTTACGATCTCATCAAGACCCTGAAGACAGCTAAAATTA GAGTGTCTGTTATTGGATTATCTGCGGAGGTTCGAGTTTGTACTGTACTTGCTCGTGAAACTGGTGGCAC ATACCATGTTATCTTAGATGAAACCCATTACAAGGAGTTGTTGGCACATCATGTGAGCCCCCTTCCTGCC AGCTCAAGCTCCGAGTGCTCACTCATTCGCATGGGATTCCCTCAGCATACCATTGCTTCTTTGTCTGATC AGGATGCAAAACCATCCTTCAGCATGGCGCATTTGGATAACAACAGCACTGAGCCAGGGCTTACACTGGG AGGCTACTTCTGC
Notes:	The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:AGCTGAGGACCGAGAGTTGA; Reverse Primer - name:unspecified, sequence:GCAGAAGTAGCCTCCCAGTG. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13754 same embryo
	EMAGE:13753 same embryo
	EMAGE:13752 same embryo
	EurExpress:euxassay_019537 same experiment
	MGI:4825274 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source