EMAGE mouse gene expression spatial database

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EMAGE:13890

Slc3a2 solute carrier family 3 (activators of dibasic and neutral amino acid transport), member 2 ( MGI:96955)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007752. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007752_01	euxassay_007752_02	euxassay_007752_03	euxassay_007752_04

euxassay_007752_05	euxassay_007752_06	euxassay_007752_07	euxassay_007752_08	euxassay_007752_09

euxassay_007752_10	euxassay_007752_11	euxassay_007752_12	euxassay_007752_13	euxassay_007752_14

euxassay_007752_15	euxassay_007752_16	euxassay_007752_17	euxassay_007752_18	euxassay_007752_19

euxassay_007752_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13890_wholemount_strong.wlz

13890_wholemount_moderate.wlz

13890_wholemount_weak.wlz

13890_wholemount_possible.wlz

13890_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13890_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

strong

regional

strong expression: see section 08 09 12 moderate expression: see section 11

submandibular gland primordium

strong

regional

strong expression: see section 05 06 07 08 13 14 15 16

vibrissa

strong

regional

strong expression: see section 04 05 06 07 20

diencephalon lateral wall mantle layer

strong

single cell

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16

diencephalon meninges

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 14 15 16

telencephalon mantle layer

strong

single cell

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

telencephalon meninges

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

medulla oblongata alar plate mantle layer

strong

single cell

strong expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16

medulla oblongata basal plate mantle layer

strong

single cell

strong expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16

hindbrain meninges

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18

rest of cerebellum mantle layer

strong

single cell

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18

pons mantle layer

strong

single cell

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18

midbrain meninges

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16

midbrain mantle layer

strong

single cell

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16

spinal cord mantle layer

strong

single cell

strong expression: see section 05 06 07 08 09 10 11 12

spinal cord meninges

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13

midgut

strong

regional

strong expression: see section 09 10 11 12 13 14 15

lower jaw incisor

strong

regional

strong expression: see section 10 13

lower jaw molar

strong

regional

strong expression: see section 05 06 07 16 17

upper jaw incisor

strong

regional

strong expression: see section 10

upper jaw molar

strong

regional

strong expression: see section 05 06 07 16 17

liver

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1413
Entity Detected:	Slc3a2, solute carrier family 3 (activators of dibasic and neutral amino acid transport), member 2 ( MGI:96955)
Sequence:	sense strand is shown >T1413 TCCTCGAGACTGTTGNCCTACTGGAGAAGCAGTTAGGAAGCTCTGCTAGCCTCACGGCCACGGGACGCCT CTCTGAACGGGGATCCAGGCAGGATTAGAGCTGCCTCACTGACTACAGGCCGTGTCGTGTCACCGTTTCT GCAGGCACCATGAGCCAGGACACCGAAGTGGACATGAAAGATGTGGAGCTGAACGAGCTAGAACCGGAGA AGCAGCCCATGAATGCAGCGGACGGGGCGGCGGCCGGGGAGAAGAACGGTCTGGTGAAGATCAAGGTGGC GGAGGACGAGACGGAGGCCGGGGTCAAGTTCACCGGCTTATCCAAGGAGGAGCTACTGAAGGTAGCGGGC AGCCCTGGCTGGGTGCGCACCCGCTGGGCGCTGCTGCTGCTCTTCTGGCTCGGTTGGCTGGGCATGCTGG CGGGCGCCGTGGTTATCATCGTTCGGGCGCCGCGCTGCCGTGAGCTGCCTGTACAGAGGTGGTGGCACAA GGGCGCCCTCTACCGCATCGGCGACCTTCAGGCCTTTGTAGGCCGGGATGCGGGAGGCATAGCTGGTCTG AAGAGCCATCTGGAGTACTTGAGCACCCTGAAGGT
Notes:	The probe template was PCR amplified from IMAGE:2373227 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2373227 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	MGI:4828227 same experiment
	EurExpress:euxassay_007752 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source