EMAGE mouse gene expression spatial database

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EMAGE:13962

Fnbp1l formin binding protein 1-like ( MGI:1925642)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007867. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007867_01	euxassay_007867_02	euxassay_007867_03	euxassay_007867_04

euxassay_007867_05	euxassay_007867_06	euxassay_007867_07	euxassay_007867_08	euxassay_007867_09

euxassay_007867_10	euxassay_007867_11	euxassay_007867_12	euxassay_007867_13	euxassay_007867_14

euxassay_007867_15	euxassay_007867_16	euxassay_007867_17	euxassay_007867_18	euxassay_007867_19

euxassay_007867_20	euxassay_007867_21	euxassay_007867_22	euxassay_007867_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13962_wholemount_strong.wlz

13962_wholemount_moderate.wlz

13962_wholemount_weak.wlz

13962_wholemount_possible.wlz

13962_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13962_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon

moderate

homogeneous

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17

pituitary gland

moderate

regional

moderate expression: see section 12 weak expression: see section 10 11 13 14 15

telencephalon

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

hindbrain

moderate

homogeneous

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

midbrain

moderate

homogeneous

moderate expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 17 18 weak expression: see section 07

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 17 18 20 weak expression: see section 07 08 16 19

vagus x ganglion

weak

regional

weak expression: see section 08 16

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 18 weak expression: see section 07

spinal cord

moderate

homogeneous

moderate expression: see section 08 09 10 11 12 13 14 15 16

dorsal root ganglion

moderate

regional

moderate expression: see section 07 08 09 14 15 16 17 18 weak expression: see section 12 13

neural retina

moderate

regional

moderate expression: see section 01 02 03 20 21 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T3082
Entity Detected:	Fnbp1l, formin binding protein 1-like ( MGI:1925642)
Sequence:	sense strand is shown >T3082 TTACGCTAAAGCTGAATGACAATTGTGTGAACATTAATGCCTTCATAAGATCAAGTACACCACTGTTATG CAGCTGACATATAGTATATTACATTTGAGGCTAGTAAACTATACAGTTTTAGATATTAAATCTCGTTACA GGGTTATTTATATAATGTGACATTATTCTATACTGACAGACTACATGAAGTAGTTTTAAAAAGTAGTGCT ATTTTTATTTCAAAAGTTAGCAATGAGAAGGAAATGTGACCTGGCTGTGTTTGTCTTCTGTACAGAGACT GAAGTGCTTATGTTTTTCTGACTAACAATCACAGAGGGCAAAGTTCAACGCTTTCTTGTACAGACTGTTC TTTTCAAGCTACTGTGTGTTTTTCTAAAGCTGGTGTTTGCTTCCATAGTAGGCAAGTTCCTTCATGTGGA GTAGTGCAACCTGTGTATGGGTTATAACATTTGAAGACACTTGGACTTGCACAGTTTTTAAGTCATTCTT AAACCATTCTTAAACTTTGAACATGTGAGCTGTCT
Notes:	The probe template was PCR amplified from IMAGE:1546894 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1546894 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13966 same embryo
	EMAGE:13963 same embryo
	EMAGE:13967 same embryo
	EMAGE:13964 same embryo
	EMAGE:13965 same embryo
	EurExpress:euxassay_007867 same experiment
	MGI:4824890 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source