EMAGE mouse gene expression spatial database

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EMAGE:14112

Preb prolactin regulatory element binding ( MGI:1355326)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001791. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001791_01	euxassay_001791_02	euxassay_001791_03	euxassay_001791_04

euxassay_001791_05	euxassay_001791_06	euxassay_001791_07	euxassay_001791_08	euxassay_001791_09

euxassay_001791_10	euxassay_001791_11	euxassay_001791_12	euxassay_001791_13	euxassay_001791_14

euxassay_001791_15	euxassay_001791_16	euxassay_001791_17	euxassay_001791_18	euxassay_001791_19

euxassay_001791_20	euxassay_001791_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14112_wholemount_strong.wlz

14112_wholemount_moderate.wlz

14112_wholemount_weak.wlz

14112_wholemount_possible.wlz

14112_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14112_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

moderate

regional

moderate expression: see section 01 02 12 13 14 15 16 17 18

meckel's cartilage

moderate

regional

moderate expression: see section 03 04 05 06 07 13 14 15 16 17 18 19

lower jaw incisor

moderate

regional

moderate expression: see section 08 09 10

lower jaw molar

moderate

regional

moderate expression: see section 05 06 07 15 16

upper jaw incisor

moderate

regional

moderate expression: see section 08 09 10

upper jaw molar

moderate

regional

moderate expression: see section 05 06 07 08 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4405
Entity Detected:	Preb, prolactin regulatory element binding ( MGI:1355326)
Sequence:	sense strand is shown >T4405 CGGACGCGTGGGTGAGAGGGGTCGGCAGTCTTCCGGAAAACTCTAGCGCCAAGTTCCTGAGCTTTCGGAG GCGAGGCGCGGCATGTCGTGGGTTCCGCGGGGTTGGCGGTGAACGTGCGGGCGGGATGGGTCGGCGCCGG GGTGTGGAGCTGTACCGGGCCCCGTTCCCGTTGTACGCGCTTCGGATAGACCCCAAGACTGGGCTGCTCA TCGCTGCGGGCGGAGGAGGAGCTGCCAAGACCGGCATAAAGAATGGCGTGCATTTTCTGCAGCTAGAGCT GATCAACGGGTGCCTGAGCGCTTCCTTGCTGCACTCTCATGACACGGAGACACGGGCCACCATGAATTTG GCGCTTGCTGGTGACATTCTTGCTGCCGGACAGGATGCCCAGTGTCAGCTTCTTCGTTTCCAGGTCCATC AACAGAAGGGCAGTAAAGCGGAGAAGTCAGGTTCCAAGGAGCAGGGACCTCGACAGAGAAAGGGGGCTCC TCCAGCAGAGAAGAAATCGGGAGCACAAGTTCACCCCGAAGGGGTTGAACTCAAAGTAAAGAATTTGGAG GCAGTACAGACAGACTTCAGCAATGAACCGCTGCAGAAAGTTGTGTGCTTCAACCATGATAACACCCTGC TTGCCACTGGAGGAACTGATGGTCATGTTCGTGTCTGGAAGGTACCTAGCCTGGAGAAAGTTCTGGAGTT CAAAGCCCATGAAGGGGAGATTGGAGATTTGACTTTGGGTCCTGATGGCAAGCTGGTTACAGTGGGCTGG GACTTTAAGGCCTCTGTGTGGCAGAAGGATCAACTGGTGACACAGCTACAGTGGCAAGAGAATGGACCAG CCTCTTCTAATACACCATACCGCTACCAGGCCTGCAGGTTTGGGCAGGTTCCAGATCAGCTCGGTGGGCT GCGACTCTTCACAGTGCAGATACCCCACAAGCGCCTACGTCAGCCCCCACCCTGCTACCTCACAGCCTGG GACAGTTCCACCTTCTTGCCTCTTCGGACCAGATCCTGTGGCCATGAGGTCATTTCCTGCCTCAGTGTCA GTGATTCGGGTACCTTCCTAGGCCTAGGCACGGTCACTGGCTCTGTCGCCATCTACATAGCTTTCTCTCT CCAGCGCCTGTATTATGTGAAGGAGGCCCATGGCATTGTGGTAACAGATGTGACCTTTCTACCTGAGAAG GGTTGTGGTCCAAAGCTCCTTGGGCCCCATGAAACTGCTCTTTTCTCTGTGGCTGTGGATAGTCGTTGCC AGTTGCACCTGCTGCCTTCACGGCGGAGTGTTCCTGTATGGCTTCTGCTCCTGCTGTGTGTCGGCCTTAT TATTGTGACCATCCTGCTGCTCCAGACTGCCTTCCCGGGATTTCTCTAACCTCTAGAGCGATGGGAATCA GCCTTGGACAGTGCCGCCTTCCAGAGCAGAATCACTGAGCCCCACGGCTGAAGCTGCATCTGATGAAACT GATGGGTACTGCTTGTCCCTCAGCTCACACTTGGCGGTGGCCTCTCGATCACTCTGGGTCTTGGGAGCTC TTGCCTTCACCTGTGGATCCACTTAAGACATTGTGGTCTGAAGCTCAGGCCACAGTGCCTGCCTCGTTCC CTCAGCCTCCCTCCCAGGGCTCCAGAGCTCAGCTCTTCCTTTTCTAGAAGCATGTGAAGATGCCCAAGAG CCTGGAGACATTGCCATCCTTCCTACAGAAACTGTTTCTCCTCCTCCCCTCCAGCCTTCACACAAGGGCC TTGGCTTTGTTGAGACAGAGCCTGTGCTGGCACTTGCTGTTAGGGTAGGAAGAGGCACAAAGAGGACCCC GGTAGCGGTGATCTGTGGCCTTCTGTGGGTAGCACCAAGCCAGTCAGTCCAGACACATAGTGAGTTCCTG GAGGGTCTACTGCAGCCTGAGGAAAGGGAACCTCAGCTCGTTAGGCAGGAAGAGTTGATATTTAATAAAG AGAGAACAATTGAACCGAGAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4921119 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4921119 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	MGI:4827404 same experiment
	EurExpress:euxassay_001791 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source