EMAGE mouse gene expression spatial database

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EMAGE:14167

C3 complement component 3 ( MGI:88227)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001854. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001854_01	euxassay_001854_02	euxassay_001854_03	euxassay_001854_04

euxassay_001854_05	euxassay_001854_06	euxassay_001854_07	euxassay_001854_08	euxassay_001854_09

euxassay_001854_10	euxassay_001854_11	euxassay_001854_12	euxassay_001854_13	euxassay_001854_14

euxassay_001854_15	euxassay_001854_16	euxassay_001854_17	euxassay_001854_18	euxassay_001854_19

euxassay_001854_20	euxassay_001854_21	euxassay_001854_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14167_wholemount_strong.wlz

14167_wholemount_moderate.wlz

14167_wholemount_weak.wlz

14167_wholemount_possible.wlz

14167_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14167_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

liver lobe

strong

homogeneous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 moderate expression: see section 20 21 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4954
Entity Detected:	C3, complement component 3 ( MGI:88227)
Sequence:	sense strand is shown >T4954 CTGCCCCTTACCCCTTCATTCCTTCCACCTTTTTCCTTCACTATGGGACCAGCTTCAGGGTCCCAGCTAC TAGTGCTACTGCTGCTGTTGGCCAGCTCCCCATTAGCTCTGGGGATCCCCATGTATTCCATCATTACTCC CAATGTCCTACGGCTGGAGAGCGAAGAGACCATCGTACTGGAGGCCCACGATGCTCAGGGTGACATCCCA GTCACAGTCACTGTGCAAGACTTCCTAAAGAGGCAAGTGCTGACCAGTGAGAAGACAGTGTTGACAGGAG CCAGTGGACATCTGAGAAGCGTCTCCATCAAGATTCCAGCCAGTAAGGAATTCAACTCAGATAAGGAGGG GCACAAGTACGTGACAGTGGTGGCAAACTTCGGGGAAACGGTGGTGGAGAAAGCAGTGATGGTAAGCTTC CAGAGTGGGTACCTCTTCATCCAGACAGACAAGACCATCTACACCCCTGGCTCCACTGTCTTATATCGGA TCTTCACTGTGGACAACAACCTACTGCCCGTGGGCAAGACAGTCGTCATCCTCATTGAGACCCCCGATGG CATTCCTGTCAAGAGAGACATTCTGTCTTCCAACAACCAACACGGCATCTTGCCTTTGTCTTGGAACATT CCTGAACTGGTCAACATGGGGCAGTGGAAGATCCGAGCCTTTTACGAACATGCGCCGAAGCAGATCTTCT CCGCAGAGTTTGAGGTGAAGGAATACGTGCTGCCCAGTTTTGAGGTCCGGGTGGAGCCCACAGAGACATT TTATTACATCGATGACCCAAATGGCCTGGAAGTTTCCATCATAGCCAAGTTCCTGTACGGGAAAAACGTG GACGGGACAGCCTTCGTGATTTTTGGGGTCCAGGATGGCGATAAGAAGATTTCTCTGGCCCACTCCCTCA CGCGCGTAGTGATTGAGGATGGTGTGGGGGATGCAGTGCTGACCCGGAAGGTGCTGATGGAGGGGGTACG GCCTTCCAACGCCGACGCCCTGGTGGGGAAGTCCCTGTATGTCTCCGTCACTGTCATCCTGCACTCAGGT AGTGACATGGTAGAGGCAGAGCGCAGTGGGATCCCGATTGTCACTTCCCCGTACCAGATCCACTTCACCA AGACACCCAAATTCTTCAAGCCAGCCATGCCCTTTGACCTCATGGTGTTCGTGACCAACCCCGATGGCTC TCCGGCCAGCAAAGTGCTGGTGGTCACTCAGGGATCTAATGCAAAGGCTCTCACCCAAGATGATGGCGTG GCCAAGCTAAGCATCAACACACCCAACAGCCGCCAACCCCTGACCATCACAGTCCGCACCAAGAAGGACA CTCTCCCAGAATCACGGCAGGCCACCAAGACAATGGAGGCCCATCCCTACAGCACTATGCACAACTCCAA CAACTACCTACACTTGTCAGTGTCACGAATGGAGCTCAAGCCGGGGGACAACCTCAATGTCAACTTCCAC CTGCGCACAGACCCAGGCCATGAGGCCAAGATCCGATACTACACCTACCTGGTTATGAACAAGGGGAAGC TCCTGAAGGCAGGCCGCCAGGTTCGGGAGCCTGGCCAGGACCTGGTGGTCTTGTCCCTGCCCATCACTCC AGAGTTTATTCCTTCATTTCGCCTGGTGGCTTACTACACCCTGATTGGAGCTAGTGGCCAGAGGGAGGTG GTGGCTGACTCTGTGTGGGTGGATGTGAAGGATTCCTGTATTGGCACGCTGGTGGTGAAGGGTGACCCAA GAGATAACCATCTCGCACCTGGGCAACAAACGACACTCAGGATTGAAGGAAACCAGGGGGCCCGAGTGGG GCTAGTGGCTGTGGACAAGGGAGTGTTTGTGCTGAACAAGAAGAACAAACTCACACAGAGCAAGATCTGG GATGTGGTAGAGAAGGCAGACATTGGCTGCACCCCAGGCAGTGGGAAGAACTATGCTGGTGTCTTCATGG ATGCAGGCCTGGCCTTCAAGACAAGCCAAGGACTGCAGACTGAACAGAGAGCAGATCTTGAGTGCACCAA GCCAGCAGCCCGCCGCCGTCGCTCAGTACAGTTGATGGAAAGAAGGATGGACAAAGCTGGTCAGTACACT GACAAGGGTCTTCGGAAGTGTTGTGAGGATGGTATGCGGGATATCCCTATGAGATACAGCTGCCAGCGCC GGGCACGCCTCATCACCCAGGGCGAGAACTGCATAAAGGCCTTCATAGACTGCTGCAACCACATCACCAA GCTGCGTGAACAACACAGAAGAGACCACGTGCTGGGCCTGGCCAGGAGTGAATTGGAGGAAGACATAATT CCAGAAGAAGATATTATCTCTAGAAGCCACTTCCCACAGAGCTGGTTGTGGACCATAGAAGAGTTGAAAG AACCAGAGAAAAATGGAATCTCTACGAAGGTCATGAACATCTTTCTCAAAGATTCCATCACCACCTGGGA GATTCTGGCAGTGAGCTTGTCAGACAAGAAAGGGATCTGTGTGGCAGACCCCTATGAGATCAGAGTGATG CAGGACTTCTTCATTGACCTGCGGCTGCCCTACTCTGTAGTGCGCAACGAACAGGTGGAGATCAGAGCTG TGCTCTTCAACTACCGTGAACAGGAGGAACTTAAGGTGAGGGTGGAACTGTTGCATAATCCAGCCTTCTG CAGCATGGCCACCGCCAAGAATCGCTACTTCCAGACCATCAAAATCCCTCCCAAGTCCTCGGTGGCTGTA CCGTATGTCATTGTCCCCTTGAAGATCGGCCAACAAGAGGTGGAGGTCAAGGCTGCTGTCTTCAATCACT TCATCAGTGATGGTGTCAAGAAGACACTGAAGGTCGTGCCAGAAGGAATGAGAATCAACAAAACTGTGGC CATCCATACACTGGACCCAGAGAAGCTCGGTCAAGGGGGAGTGCAGAAGGTGGATGTGCCTGCCGCAGAC CTTAGCGACCAAGTGCCAGACACAGACTCTGAGACCAGAATTATCCTGCAAGGGAGCCCGGTGGTTCAGA TGGCTGAAGATGCTGTGGACGGGGAGCGGCTGAAACACCTGATCGTGACCCCCGCAGGCTGTGGGGAACA GAACATGATTGGCATGACACCAACAGTCATTGCGGTACACTACCTGGACCAGACCGAACAGTGGGAGAAG TTCGGCATAGAGAAGAGGCAAGAGGCCCTGGAGCTCATCAAGAAAGGGTACACCCAGCAGCTGGCCTTCA AACAGCCCAGCTCTGCCTATGCTGCCTTCAACAACCGGCCCCCCAGCACCTGGCTGACAGCCTACGTGGT CAAGGTCTTCTCTCTAGCTGCCAACCTCATCGCCATCGACTCTCACGTCCTGTGTGGGGCTGTTAAATGG TTGATTCTGGAGAAACAGAAGCCGGATGGTGTCTTTCAGGAGGATGGGCCCGTGATTCACCAAGAAATGA TTGGTGGCTTCCGGAACGCCAAGGAGGCAGATGTGTCACTCACAGCCTTCGTCCTCATCGCACTGCAGGA AGCCAGGGACATCTGTGAGGGGCAGGTCAATAGCCTTCCTGGGAGCATCAACAAGGCAGGGGAGTATATT GAAGCCAGTTACATGAACCTGCAGAGACCATACACAGTGGCCATTGCTGGGTATGCCCTGGCCCTGATGA ACAAACTGGAGGAACCTTACCTCGGCAAGTTTCTGAACACAGCCAAAGATCGGAACCGCTGGGAGGAGCC TGACCAGCAGCTCTACAACGTAGAGGCCACATCCTACGCCCTCCTGGCCCTGCTGCTGCTGAAAGACTTT GACTCTGTGCCCCCTGTAGTGCGCTGGCTCAATGAGCAAAGATACTACGGAGGCGGCTATGGCTCCACCC AGGCTACCTTCATGGTATTCCAAGCCTTGGCCCAATATCAAACAGATGTCCCTGACCATAAGGACTTGAA CATGGATGTGTCCTTCCACCTCCCCAGCCGTAGCTCTGCAACCACGTTTCGCCTGCTCTGGGAAAATGGC AACCTCCTGCGATCGGAAGAGACCAAGCAAAATGAGGCCTTCTCTCTAACAGCCAAAGGAAAAGGCCGAG GCACATTGTCGGTGGTGGCAGTGTATCATGCCAAACTCAAAAGCAAAGTCACCTGCAAGAAGTTTGACCT CAGGGTCAGCATAAGACCAGCCCCTGAGACAGCCAAGAAGCCCGAGGAAGCCAAGAATACCATGTTCCTT GAAATCTGCACCAAGTACTTGGGAGATGTGGACGCCACTATGTCCATCCTGGACATCTCCATGATGACTG GCTTTGCTCCAGACACAAAGGACCTGGAACTGCTGGCCTCTGGAGTAGATAGATACATCTCCAAGTACGA GATGAACAAAGCCTTCTCCAACAAGAACACCCTCATCATCTACCTAGAAAAGATTTCACACACCGAAGAA GACTGCCTGACCTTCAAAGTTCACCAGTACTTTAATGTGGGACTTATCCAGCCCGGGTCGGTCAAGGTCT ACTCCTATTACAACCTCGAGGAATCATGCACCCGGTTCTATCATCCAGAGAAGGACGATGGGATGCTCAG CAAGCTGTGCCACAGTGAAATGTGCCGGTGTGCTGAAGAGAACTGCTTCATGCAACAGTCACAGGAGAAG ATCAACCTGAATGTCCGGCTAGACAAGGCTTGTGAGCCCGGAGTCGACTATGTGTACAAGACCGAGCTAA CCAACATAGAGCTGTTGGATGATTTTGATGAGTACACCATGACCATCCAGCAGGTCATCAAGTCAGGCTC AGATGAGGTGCAGGCAGGGCAGCAACGCAAGTTCATCAGCCACATCAAGTGCAGAAACGCCCTGAAGCTG CAGAAAGGGAAGAAGTACCTCATGTGGGGCCTCTCCTCTGACCTCTGGGGAGAAAAGCCCAACACCAGCT ACATCATTGGGAAGGACACGTGGGTGGAGCACTGGCCTGAGGCAGAAGAATGCCAGGATCAGAAGTACCA GAAACAGTGCGAAGAACTTGGGGCATTCACAGAATCTATGGTGGTTTATGGTTGTCCCAACTGACTACAG CCCAGCCCTCTAATAAAGCTTCAGTTGTATTTCAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5134713 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5134713 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14165 same embryo
	EMAGE:14168 same embryo
	EMAGE:14166 same embryo
	EMAGE:14164 same embryo
	EurExpress:euxassay_001854 same experiment
	MGI:4823553 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source