EMAGE mouse gene expression spatial database

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EMAGE:14253

Abi3 ABI gene family, member 3 ( MGI:1913860)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004063. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004063_01	euxassay_004063_02	euxassay_004063_03	euxassay_004063_04

euxassay_004063_05	euxassay_004063_06	euxassay_004063_07	euxassay_004063_08	euxassay_004063_09

euxassay_004063_10	euxassay_004063_11	euxassay_004063_12	euxassay_004063_13	euxassay_004063_14

euxassay_004063_15	euxassay_004063_16	euxassay_004063_17	euxassay_004063_18	euxassay_004063_19

euxassay_004063_20	euxassay_004063_21	euxassay_004063_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14253_wholemount_strong.wlz

14253_wholemount_moderate.wlz

14253_wholemount_weak.wlz

14253_wholemount_possible.wlz

14253_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14253_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

regional

moderate expression: see section 09 weak expression: see section 08 10 11 12

heart ventricle

weak

regional

weak expression: see section 08 09 10 11 12 13 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5558
Entity Detected:	Abi3, ABI gene family, member 3 ( MGI:1913860)
Sequence:	sense strand is shown >T5558 GCCATGGCGGAGCTGCAGCAGTTACAGCAGCTGCAGGAGTTTGATATCCCCACGGGCCGGGAGGCTCTGC GGGGCAACCACAGCGCCCTGCTACGGGTGGCCAACTACTGCGAGGATAACTACTTGCAGGCCACAGACAA GCGGAAGGCGCTGGAAGAGACGATGGCTTTCACCACCCAGGCCCTAGCCAGTGTGGCCTACCAAGTGGGT AACCTGGCCGGGCATACTCTTCGAATGCTGGATCTACAGGGTGCTGCCCTGCGGCAGGTAGAAGCCAAGA TGAGCACACTGGGCCAGATGGTAAACATGCATATGGAGAAGGTAGCTAGAAGGGAGATCGGCACTTTGGC CACTGTTGTGCGGCTGCCCTCTAACCAGAAAGTCATCCCTCCTGAGAGCCTGCCTTCCCTCACTCCCTAC CACAGAAAACCCCTCAACTTTGCCTGCTTGGATGACATTGGCCACGGAGTCAAGGACTTGAGCACGCAGC TGTCACGGACCGGGACCCTGTCTCGCAAGAGCATAAAGGCGCCTGCTACACCTGTTTCCGCCACGCTGGG GAGACCACCCCGGATCCCTGAGCCGGTGCAGCTCCCAGCCGTGCCAGACGGCAAGCTCTCCGCTGCCTCC TCTGCCTCTTCCTTGGCCTCGGCAGGCAGTGCGGAAGGTGCCAGTGGGATCCCCCAGTCCAAGGGACAGG TAGCGCCTGCAACCCCGCCTCCTCCACCTGTAGCACCTGTAACCCCGCCTCCTCCACCGTTGTCTGCCGA GGTCTTCTTGCCGCCCCCTCCGCTGGAGGTGTCCCAGCCCCCTCTGGAAGCAGAGTTGCCCCTGCCTCCT CCCCCAGCTCTAGAGGGGGATGAACTGGGGCTGCTGCCTCCGCCCCCACCAGGTTTTGGACCAGATGAGC CCAGCTGGGTCCCTGCTAGCTACTTGGAGAAAGTGGTGACCCTGTACCCATACACCCGGCAAAAGGACAA TGAGCTCTCCTTCTCTGAAGGCACCGTCATCTGTGTCATTCGCCGCTACTCGGATGGCTGGTGTGAGGGT GTCAGTTCAGAGGGCACTGGATTCTTCCCAGGGAACTATGTGGAGCCCAGCTGCTGACAGCCCAGGATCT ATCCCTGCACCAACCCTGCACTGCCTCGGTGGGCCTCTCGAGCCCCAGGAAGCCAGCTTCCACAGTCAAA GCTGGACTAAGGACCTGTCTACCTCTTGGGCTGTGAACTGTGTTCAGTCCCTGCTCCATACAGCAGTGGG AAGAGGGTGGGCTAGAGAGGGGTGGTACTCAGGGGGGACTGCTGCAGATGGCATAAAGAATACAAAAACA AACAAACCTAGAAGGCAAGGAGTTTAAGCACCTTGCCCAGAGGACCCCCAGCTCATGCTCAGAAGATCGC CAGCTTTGAATAAAACTGCTGACCTCCAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6474610 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6474610 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14250 same embryo
	EMAGE:14249 same embryo
	EMAGE:14252 same embryo
	EMAGE:14248 same embryo
	EMAGE:14251 same embryo
	EurExpress:euxassay_004063 same experiment
	MGI:4822882 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source