EMAGE mouse gene expression spatial database

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EMAGE:14369

Clspn claspin homolog (Xenopus laevis) ( MGI:2445153)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004199. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004199_01	euxassay_004199_02	euxassay_004199_03	euxassay_004199_04

euxassay_004199_05	euxassay_004199_06	euxassay_004199_07	euxassay_004199_08	euxassay_004199_09

euxassay_004199_10	euxassay_004199_11	euxassay_004199_12	euxassay_004199_13	euxassay_004199_14

euxassay_004199_15	euxassay_004199_16	euxassay_004199_17	euxassay_004199_18	euxassay_004199_19

euxassay_004199_20	euxassay_004199_21	euxassay_004199_22	euxassay_004199_23	euxassay_004199_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14369_wholemount_strong.wlz

14369_wholemount_moderate.wlz

14369_wholemount_weak.wlz

14369_wholemount_possible.wlz

14369_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14369_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

olfactory cortex ventricular layer

moderate

homogeneous

moderate expression: see section 09 10 11 14 15 16

telencephalon ventricular layer

moderate

homogeneous

moderate expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 24 weak expression: see section 04

midbrain ventricular layer

weak

regional

weak expression: see section 11 12 13 14 15 16 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7883
Entity Detected:	Clspn, claspin homolog (Xenopus laevis) ( MGI:2445153)
Sequence:	sense strand is shown >T7883 CGAGCTGGAGACGGCGGGAGCTGTCGCCGTCAAGTTGAGGTTACTGCAGACACGTTCTCCTGGAGCTGTG CGGAAGGGAGGCCGGAATCATGACCGGCGAGGTGGGTTCTGAGGTTAACCTGGAAGTCAACGATCTAAAA CTTCTTTCACAAGAAGCAGCAGACAGTCCTGTAGACAGTGGACAGGGCAGCTTTGAAACACTCGAGCCCT TGAGTGAAAGAGATTCTGATGAAGAGATTTTTGTGAGCAAGAAACCTAAGAGCAGGAAAGTGTTACAAGA CAGCGATTCTGAGGCTGAGGACAGAGACGATGCTCCGGAGAAACCCACCTACGACGACAGCGCAGAGGAC ACTCAGGAGAACTTACATTCAGGGAAGAGTCAGAGCAGAAGCTTTCCCAAAGCCCTGGCTGACAGTGATG AGAGTGACATGGAGGAAACTCCGAGTCAGGAGAGTCCTGAGACTCAAGAGGCTCCTTCCTTGGAGCCAGG CCACCAGACAGGACACTCTGTGGACTTCACCACCGGCAGAAAGCTCTCCAAAACACTCCTCAGAGAAGGA GCTGAGGGAAAAGCAAAATCCAAGAGACGGCTGGAGAAAGAGGAGAGAACAATGGAGAAAATCAGGAGAC TAAAAAAGAAGGAAACAAGATGTGAGGAAAGTGATGCAGACCGGCCGCTTAATGACAGTGGCTGTCTCCT TGAAGATAGTGATCTTTTTGAGACAGGTTTGGAGGAAGAAAATGACTCTGCACTGGAGGATGAAGAGTCG TTAGAGTCAATAAGGGCGGCTGTAAAAAACAAGGTGAAAAATCGCAAGAAAAAGGAACCAACTCTGGAGA GTGAGGCTTTTTCATTAGAAGACGGAAATGAGTTATCCAAAGGCAGTGCGAGGAAGGAAAGAAAGGCAGC GAGGCTAAGTAAGGAAGCATTAAAAAAACTGCATAGTGAGACCCAGCGTCTGGTCCGAGAATCTGCACTT AATCTTCCATATCATATGCCTGAGAGTAAAACTATTCACGATTTCTTCAAACGTAAGCCCCGGCCCACGT GCCAGGGCAGTGCCATGGCCCTGCTGAAGTCATGTAAGTATCAGTCGGGCCATTACAAGGAAACCGTAAA CCCTGCAGATGCAGCTGGAATGGGTGCTGAGGACTCCAGCAGAGGTTCTGAGCAGAGGACAGGGGCTGGA ATTGCAGCGGAAACTAATGTTCTCTCTGAGGTCTCAGAAGAAGCCGGGATCACTGCTGGATCAGATGAGG CTTGTGGGAAGGATCCGGTAAGACGAGGAGAGCTGGAAATTGAGGAGACTGAGAAGCACAGTGATGACAG ACCTTATTCTCCTGGGGACAGATCCATGTCACAGCAGGAATCCTCCATCCCCAGGATCGAAGACAATGAG GGGCATCAGGCTGGAGACCTCACCGAATCTGACCCTCCTGCCCTGGAGGGAGAAGAACTGAAAACAGTAG AAAAAACAGACGCAAAAGAGGGAATGCCTGAACAGAAAACCCAGTCTGCAGCAGCAGCGGCAGTGGCAGT AGTGACGGCGGCGGCGGCGCCACCTGAAAAGGTGAGACGATTCACCGTGGACCGACTTAGACAGCTGGGA GTGGATGTTTCCAGTCAACCTCGGCTGGGCGCTGATGAAGATTCCTTTGTGATACTTGATGAACCTAAAA CCAACAGAGAATTGGAAGCCTTGAAGCAACGTTTCTGGAGGCACGCTAACCCAGCAGCCAGTCCCAGGGC TTGTCAGACGGTGAACGTGAACATCATCGTGAAGGACTTGGGCACTAACGGGAAGGAGGAGCTGAAGGCA GAAGTGGTACCCGTCACTTTGGCAGCAGAGAAGCTGGAGGGAGCAAGCCACGCAAAGCCAGGTGAAAAAC TGCAGATGTTGAAAGCTAAGTTGCAGGAGGCAATGAAACTCCGAAGGCTTGAGGAGCGGCAGAAGCGCCA AGCATTATTTAAATTAGATAATGAAGATGGATTTGAAGAGGAGGAGGAAGAGGAAGAAATGACAGATGAG TCTGAGGAAGATGGAGAAGAGGAGACTACAGAATATCTTCTTGGTAGTGAAGATACAGAAACCAAAGATG AAAAAGAAACAGATAAAGAAAATACAGACACCAGTAGTGACATTGGCAAGTCTGTTGCCCTCTGTGTCCC CAAGCCTCTCTCTTCAGACTCCACCTTACTTCTGTTTAAGGACAGCTCTTCTAAGATGGGTTACTTTCCT ACTGAAGAAAAGTCAGAAACAGATGAACACTTGGCCAAGCAGTCTGACAAACTGGATGAGGATGACTCGA GTTCACTGCTAACAAAGGAGAGCAGTCACAATAGCAGCTTTGAGCTGATTGGTTCCACAATTCCATCCTA TCAGCCTTGCAACAGACAGATAGGCCGTGGGGCAAGTTTTCTCCCTACAGCAGGATTCAGATCGCCGTCC CCTGGGCTGTTCCGAGGGAGTTTGATCAGCTCGGCTTCTAAGAGTTCAGGAAAACTGTCTGAGCCTTCAC TTCCCGTAGAGGATTCCCAGGATCTGTACACCGCCTCCCCAGAGCCTAAGACACTTTTCCTAGGAGCAGG AGACTTCCAGTTCTGTTTGGAAGATGACACTCAGAGCCAACTGTTGGATGCAGATGGATTTTTAAACATT AGAAATCACCGCCATCGATATCAGGCTGTGAAGCCCCAGTTGCCACTGGCCAGCATGGACGAGAATGCCA TGGACGCCAACATGGACGAGCTGCTGGATTTGTGCACTGGACAGTTTACATCTCAGCCTGAGGAGAAATG TCAACCAAGGAAGAATGACAAAAAAGAAAACATGGAGGAACTTTTGAATCTCTGCTCGGGCAAATTTCCT ACTCAAGATGCCTCCCCGGTGGCCCCACTGGGGCTGAGGAGTCAGGAGAAGGAGAGCAGCACAGAAGATC CGATGGAAGAGGCACTTGCTCTTTGCTCCGGCTCTTTCCCAACAGACAGGGAGGAAGAGGGTGAAGAGGA GGAATTTGGAGACTTCCAGCTTGTTTCAAAGGAGAATGGATTTGCTAGTGACGAGGATGAACACAGTGAC TCCAATGATGAAGAGCTGGCCCTGGACCTGGAGGATGATGAAGAAGAGCTCCTGAAGCAGTCTGAGAAGA TGAAGCGCCAAATGAGACTGAAGAAATACTTGGAAGATGAGGCAGAGGTGTCAGGCAGTGACGTGGGAAG TGAGGACGAGTACGATGGGGAGGAGATTGATGAATACGAAGAGGACGTCATTGATGAGGTGCTTCCGTCT GATGAGGAGCTGGAGAGTCAGATCAAGAAGATACACATGAAAACCATGTTGGATGATGATAAGCGGCGAC TACGGTTATATCAAGAGAGGTACCTTGCTGATGGGGATCTACACAGCGACGGGCCTGGACGAACAAGAAA ATTCCGATGGAAACACATAGATGATACCTCCCAGATGGATCTATTTCACAGAGACTCCGATGACGATCAG GTTGAAGAACAGCTTGACGAGACCGAAGCCAAGTGGAGGAAGGAACGGATTGAGCGAGAGCAGTGGCTTC GGGAACAGGCTCAACAGGGGAAAATTGCTGCTGACGAAGAGGACATTGGGGACGACAGTCAGTTTATGAT GCTAGCCAAGAAAGTCACAGCCAAAGCACTGCAGAAGAATGCCAGTCATACTGTGGTTGTTCAGGAATCA AAGTCTGTGCTCAGAAACCCTTTTGAAACCATCAGACCAGGAGGTGCTCACCAGCTGAAGACAGGCTCCC TGCTAAACCAGCCCAAAGCTGTGCTCCAAAAACTTGCTGCCCTGTCTGACCTCAACCCCAGCGCTCCCCG AAACTCAAGGAACTTTGTCTTTCATACACTTTCTCCCACCAAGGCAGAGGCAGCAAAGGACTCATCTAAG CCTCAGGTCAGAAGAAGGGGACTGTCGTCAATGATGTCACCGTCTCCTAAGCGGCTAAAGACAAACGGAA GCAGTCCAGGGCCAAAGCGAAGCATCTTCAGATACTTGGAAAGCTAACGCCATCCAGGATGCCAGTGTCT GTGGAAAGTACTCAGATTTCCTGGGTTGACACCACTTTCAAGTCAAGGTGTTCAGAGGAAGAAGAAAGTG TGGCATTATAGAAAATTGCAGAAAGCACTGAAAGCCAGCCTGGGATGAGGCTCCCACAGCCACACGGCCC TTAACACAGTACCAGGCCGAGAAGCACGCACCCAGCTGTTGCATCGTCCTGTTCCTTCTGTGTCATGTAA ACTTCTAGTCTTGATCTCTGATTAACCTTCATCGCCTTTGACATAAGGACTCAAGCTGGTACCTAGTTGA GAAAGAGGTTATCCCTCCCTTTCTGCACCTTCCCCAGGACTAGAGTTTCAACTTAAAGCATTTGTTCCAG AGCCTCCCTCCCTTCTGCAGAAACCTGACCACTATTTATTGCTGCTTTGGGGCCGAGTTTCTTGCCCTGA CCATCAGAGGACATTTTAATATTTCATCACTAATATTGAGAGCAGACCAGTAGAATTTAATAAAAACGTT GAAGAGAACTGCTATTAGTCGCTTTCTTTGGCTTTCAGAAACATAGGCCAGTTCTCTTCTATCAGCAGAA TTCAGTGCTGCTTTGCAAAAACCATGGCAGGTGAACGTTGGCTTCAGTGTCGAAAGCTGAGCGTGAGAAG TTGGATGAGGTTGTGTCCGTCCTTTATTGAGAGACATAAAGCAGCCTGTTCGACTGATGAGATTAAGATG TAGGTTTTCTGTTAGGTGCTGGGGCTTTTGGTTTGGGTTTTTCTAATACTCAGGATGCTGACTTGGACAG AACATACATTTGTATATTTGTAATCTTGTAATGGGAGAAATGTGTACAAAGCTGTTTTAATATGTATGTA GGTTTTCAATAAATCTCATTGCCTTGTGGGCAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6507692 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6507692 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14375 same embryo
	EMAGE:14371 same embryo
	EMAGE:14372 same embryo
	EMAGE:14373 same embryo
	EMAGE:14374 same embryo
	EMAGE:14370 same embryo
	EurExpress:euxassay_004199 same experiment
	MGI:4823938 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source