EMAGE mouse gene expression spatial database

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EMAGE:14735

Klf15 Kruppel-like factor 15 ( MGI:1929988)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005688. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005688_01	euxassay_005688_02	euxassay_005688_03	euxassay_005688_04

euxassay_005688_05	euxassay_005688_06	euxassay_005688_07	euxassay_005688_08	euxassay_005688_09

euxassay_005688_10	euxassay_005688_11	euxassay_005688_12	euxassay_005688_13	euxassay_005688_14

euxassay_005688_15	euxassay_005688_16	euxassay_005688_17	euxassay_005688_18	euxassay_005688_19

euxassay_005688_20	euxassay_005688_21	euxassay_005688_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14735_wholemount_strong.wlz

14735_wholemount_moderate.wlz

14735_wholemount_weak.wlz

14735_wholemount_possible.wlz

14735_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14735_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon lateral wall ventricular layer

moderate

regional

moderate expression: see section 11 12 13

telencephalon ventricular layer

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

medulla oblongata basal plate ventricular layer

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17

rest of cerebellum ventricular layer

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

pons ventricular layer

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

midbrain ventricular layer

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16

spinal cord ventricular layer

moderate

regional

moderate expression: see section 10 11

naris

moderate

regional

moderate expression: see section 11 12 14 15 16

nasal septum

moderate

regional

moderate expression: see section 13

viscerocranium

moderate

regional

Expression in the turbinate bone.

temporal bone petrous part

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 15 16 17 18 19 20 21 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T2759
Entity Detected:	Klf15, Kruppel-like factor 15 ( MGI:1929988)
Sequence:	sense strand is shown >T2759 TTCGGACGAGGCCAAGAGCAGCCACCTCAAGGCCCACCTGCGTCGGCACACAGGCGAGAAGCCCTTTGCC TGCACCTGGCCAGGCTGCGGCTGGAGGTTTTCCCGCTCAGATGAGTTGTCAAGGCACCGGCGATCTCACT CGGGTGTGAAGCCGTACCAGTGTCCCGTGTGCGAGAAGAAATTCGCGCGGAGTGACCACCTCTCCAAACA CATCAAAGTGCATCGCTTCCCACGAAGCAGCCGCGCAGTACGCGCCATCAACTGAGCGCAGTGGCCGCCC TTCCCTCCCCCAGCTCCACGTTTTGTTTTTAAATGCAATAACTTATTGCCTCTTTTCAGAAGGATGTGAC AATATTACCAGCCCCCTCCCCCTTCTGAATCTTAGGAGGTATGACCCAGAGCCACCATGGCTGCCTTTCT GGGGAAGACCTAGAGTCCCTATGGTCCCTGGGGGCTGGTTCCCCGGTGGCCCAGGTGGCCCAGGCAGGCC TGTGCCCTTGTGCCTTTGTGCCTTCCTGCCAGCTGGAAGCAGTGTTTGGGGGGCCCTTGCCCTCTTCCCA CTGGGCTC
Notes:	The probe template was PCR amplified from IMAGE:1514543 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1514543 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14733 same embryo
	EMAGE:14732 same embryo
	EMAGE:14734 same embryo
	EurExpress:euxassay_005688 same experiment
	MGI:4825781 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source