EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:14844

Zfhx4 zinc finger homeodomain 4 ( MGI:2137668)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005820. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005820_01	euxassay_005820_02	euxassay_005820_03	euxassay_005820_04

euxassay_005820_05	euxassay_005820_06	euxassay_005820_07	euxassay_005820_08	euxassay_005820_09

euxassay_005820_10	euxassay_005820_11	euxassay_005820_12	euxassay_005820_13	euxassay_005820_14

euxassay_005820_15	euxassay_005820_16	euxassay_005820_17	euxassay_005820_18	euxassay_005820_19

euxassay_005820_20	euxassay_005820_21	euxassay_005820_22	euxassay_005820_23	euxassay_005820_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14844_wholemount_strong.wlz

14844_wholemount_moderate.wlz

14844_wholemount_weak.wlz

14844_wholemount_possible.wlz

14844_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14844_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hand epidermis

moderate

regional

moderate expression: see section 01 02 03 04 23 24

foot epidermis

moderate

regional

moderate expression: see section 02 03 04 05 06 07 19 20 21 22 23 24

medulla oblongata basal plate mantle layer

strong

regional

strong expression: see section 07 08 14 15

nasal septum

moderate

regional

moderate expression: see section 13 14 15 16 17 18 weak expression: see section 11 12

urethra of male

moderate

regional

moderate expression: see section 11 12 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T35564
Entity Detected:	Zfhx4, zinc finger homeodomain 4 ( MGI:2137668)
Sequence:	sense strand is shown >T35564 TGCTTCTGGCCTAGATGGTAATTTCAATAGTGTCCGAATGGATATGTTCAGTGTGTAGGAGTGAAGACAG GATCCCGTGCTTAAAAAAAAATTTAAAAATAAAAAATTTTTAAAAAAAGACTTTAACTGCAGTTCCAAAG CTTCTCTAACCCAAAAATTACAGTACCAAATGATTGGCTCAGGATTGTTTTTCCCATATTGATATGCTGG CAATATAGGATGGTATGTGGTGAAGAAGACTAATGCAGGTGGTTGAATGCGCTTGTAATATATGCTAAAA TATGGAAAAGGAAAAAAATATCTCACAAGTTCTTTGGGAACTTGTTTCAAGCCAAAAACTCTCAAGACAG AAAATTGCACCTCAGCTGGGTTGATTTCAAAATGCCAGCATGGACAGTATGGGAGGACGATCCGGATGTG TCAAAGAGAACTTCTCTTAGTTTAGTTAGGTGTAATTCAGTAGCTTTAAATTCTCAGGTCAGAACATAAC ATTTCTCATTTGTTAAAAGCAGCAAGAAGCCTGGTAAAACTGTGACTTTTCCCCAAATGTCAATCTTTAT TAGAAAGCATTTTCTAGGTGTGTTTAGTGTGCAAAGAAACTTTCTAACCCTTACCGGACACCGCACAGCT TCCTGCACTCAAGCCGCAGGGTAGTACAGTTTTACCACAGAGGGACTCTTGAGCAATGGAGTCACGTGTC TGCCCTGTGTATTGCAGTTTGTCTTGACACAAGC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 91784. Forward Primer - name:091784_F_cDNA_Zfhx4, sequence:TGCTTCTGGCCTAGATGGTAAT; Reverse Primer - name:091784_N_SP6_cDNA_Zfhx4, sequence:GCTTGTGTCAAGACAAACTGCAA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14848 same embryo
	EMAGE:14845 same embryo
	EMAGE:14846 same embryo
	EMAGE:14843 same embryo
	EMAGE:14847 same embryo
	EurExpress:euxassay_005820 same experiment
	MGI:4829288 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source