EMAGE mouse gene expression spatial database

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EMAGE:14878

3830431G21Rik RIKEN cDNA 3830431G21 gene ( MGI:3036228)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007981. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007981_01	euxassay_007981_02	euxassay_007981_03	euxassay_007981_04

euxassay_007981_05	euxassay_007981_06	euxassay_007981_07	euxassay_007981_08	euxassay_007981_09

euxassay_007981_10	euxassay_007981_11	euxassay_007981_12	euxassay_007981_13	euxassay_007981_14

euxassay_007981_15	euxassay_007981_16	euxassay_007981_17	euxassay_007981_18	euxassay_007981_19

euxassay_007981_20	euxassay_007981_21	euxassay_007981_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14878_wholemount_strong.wlz

14878_wholemount_moderate.wlz

14878_wholemount_weak.wlz

14878_wholemount_possible.wlz

14878_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14878_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rest of cerebellum mantle layer

moderate

regional

moderate expression: see section 04 05 06 12 13 14 15

midbrain mantle layer

strong

single cell

strong expression: see section 03 04 05 06 07 09 10 11 12 moderate expression: see section 13 14

trigeminal v ganglion

strong

single cell

strong expression: see section 02 03 04 05 06 07 16 17 18 19 20

dorsal root ganglion

moderate

single cell

moderate expression: see section 01 02 03 04 05 06 07 09 10 11 12

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T35615
Entity Detected:	3830431G21Rik, RIKEN cDNA 3830431G21 gene ( MGI:3036228)
Sequence:	sense strand is shown >T35615 TCTCTCTTCGTTCCTGATCCTCCAGTACAACATTTGTCCATGCCCTGCACTTATTCCATGTTCTTGGAGA GGGAAAAGGAGGCTCTAAGATATAACCCCAGGTTCACAGCACCCCACACCCTCTCTGCAGTATTCCTGGG GGCTGTCCTGGCTGTAAGGCCACCCAGGCCTCCTATTCACAAATGGGTTTCTCCATATATAGCTCCAGCA ACCTGCTGGAGGGTGCAGGAGAAGTGTGGGATCCCACTTTGTTCTTTTCCAGCCCCGATTGAGAGTCCTT GACCCCAGGGTTTCCCAAAGTCCGCACAGCCAGATCTGGAAGAAGCTCCGGTGTTACCCACAGTCAGGCC TGTTGTGACCCTGCTGCCCTGAGAGCGCAGAGGTCTGTCTCTCTCCCAGCTGGCTCACCTTGCTGCCTCC TCTCCCTGCAGCAGACTCACTCAGTGGACCAATGCAGCCAGGAACCCACCACATGGGAACATGGGCCTGT GGGGAACTAGTAGCCATTGTTTGGTAGAGCATTGTCAGAGTGACACTGTAACACCAGCCTGCGGGTTTTA TGGAAGACCACTACCCTTCAGGAGGCCAAGGACTGAGGCGTGTGCTCCTTATCTGTGACAGTCTTTTGGG GCCCATCCCTCAGAAGCTGCCTGAGTCTATCAGTCTCGACAAACAAGACTTGGGGAGGGGGCGGAGATGT GGACAGTGGTTAA
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 83786. Forward Primer - name:083786_F_cDNA_3830431G21Rik, sequence:TCTCTCTTCGTTCCTGATCCTC; Reverse Primer - name:083786_N_SP6_cDNA_3830431G21Rik, sequence:TTAACCACTGTCCACATCTCC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14880 same embryo
	EMAGE:14881 same embryo
	EMAGE:14877 same embryo
	EMAGE:14879 same embryo
	EMAGE:14882 same embryo
	EurExpress:euxassay_007981 same experiment
	MGI:4822706 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source