EMAGE mouse gene expression spatial database

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EMAGE:14948

Bsn bassoon ( MGI:1277955)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008029. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008029_01	euxassay_008029_02	euxassay_008029_03	euxassay_008029_04

euxassay_008029_05	euxassay_008029_06	euxassay_008029_07	euxassay_008029_08	euxassay_008029_09

euxassay_008029_10	euxassay_008029_11	euxassay_008029_12	euxassay_008029_13	euxassay_008029_14

euxassay_008029_15	euxassay_008029_16	euxassay_008029_17	euxassay_008029_18	euxassay_008029_19

euxassay_008029_20	euxassay_008029_21	euxassay_008029_22	euxassay_008029_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14948_wholemount_strong.wlz

14948_wholemount_moderate.wlz

14948_wholemount_weak.wlz

14948_wholemount_possible.wlz

14948_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14948_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

facial vii ganglion

weak

regional

weak expression: see section 03 04

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 05 15 16

trigeminal v ganglion

weak

regional

weak expression: see section 03 04 05 06 07 08 16 17 18 19 20 21

vagus x ganglion

weak

regional

weak expression: see section 06

dorsal root ganglion

weak

regional

weak expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T36060
Entity Detected:	Bsn, bassoon ( MGI:1277955)
Sequence:	sense strand is shown >T36060 GATCTCCAAGTTCTCACCCATCGAGGAGGCCAAGGATGTGGAATCCGACCTGGCCTCCTATCCCCCGCCC ACCGTCAGCAGCAGCCTGACCTCTCGGGGCAGAAAGTTCCAGGATGAAATCACCTATGGGCTCAAGAAGA ACGTGTACGAACAGCAGCGGTACTACGGGGTGTCCAGCCGCGACGCGGCAGAGGAGGATGAGCGCATGTA CGGTAGCAGCAGCCGGTCCCGAATGGCGTCGGCATACAGTGGGGAGAAGCTGTCCAGCCATGACTATAGC AGCCGAGGCAAAGGGTATGAACGGGAACGGGACACTGCAGAGCGACTTCAAAAGGCGGGCTCCAAGCCCT CATCCCTGAGCATGGCCCATGGCCGGGCACGGCCCCCCATGAGGAGCCAGGCCTCTGAAGAGGAGAGCCC TGTCAGCCCCTTAGGGCGGCCCCGCCCCGCAGGGGGCGCTCTTCCTCCCGGGGACACCTGCCCACAGTTC TGCTCCAGCCATTCCATGCCTGACGTCCAGGAACATGTCAAGGACGGACCGCGGGCCCACGCGTATAAGC GTGAGGAGGGCTACATGTTGGATGATTCCCACTGCGTGGTTTCTGACAGCGAAGCGTATCAC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 97439. Forward Primer - name:097439_F_cDNA_Bsn, sequence:GATCTCCAAGTTCTCACCCATC; Reverse Primer - name:097439_N_SP6_cDNA_Bsn, sequence:GTGATACGCTTCGCTGTCAGA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14946 same embryo
	EMAGE:14950 same embryo
	EMAGE:14949 same embryo
	EMAGE:14951 same embryo
	EMAGE:14947 same embryo
	EurExpress:euxassay_008029 same experiment
	MGI:4823509 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source