EMAGE mouse gene expression spatial database

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EMAGE:14969

Bin1 bridging integrator 1 ( MGI:108092)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008004. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008004_01	euxassay_008004_02	euxassay_008004_03	euxassay_008004_04

euxassay_008004_05	euxassay_008004_06	euxassay_008004_07	euxassay_008004_08	euxassay_008004_09

euxassay_008004_10	euxassay_008004_11	euxassay_008004_12	euxassay_008004_13	euxassay_008004_14

euxassay_008004_15	euxassay_008004_16	euxassay_008004_17	euxassay_008004_18	euxassay_008004_19

euxassay_008004_20	euxassay_008004_21	euxassay_008004_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14969_wholemount_strong.wlz

14969_wholemount_moderate.wlz

14969_wholemount_weak.wlz

14969_wholemount_possible.wlz

14969_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14969_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

upper arm muscle

strong

regional

strong expression: see section 01 02 03 18 19 21 22

upper leg muscle

strong

regional

strong expression: see section 01 02 03 04 18 19 20 21

diaphragm

strong

regional

strong expression: see section 01 02 03 04 05 06 07 14 15 16 17 18 19 20 21

forearm rest of mesenchyme

strong

regional

strong expression: see section 01 02 22

shoulder joint primordium

strong

regional

strong expression: see section 22

shoulder rest of mesenchyme

strong

regional

strong expression: see section 01 02 21

hand mesenchyme

strong

regional

strong expression: see section 22 moderate expression: see section 01 02 03 18 19 20 21

foot mesenchyme

moderate

regional

moderate expression: see section 02 03 17 18 21

vertebral axis musculature

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

not examined

regional

not examined expression: see section 14

tongue muscle

moderate

regional

moderate expression: see section 10 11 12 13 14

tail mesenchyme

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 12

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T36049
Entity Detected:	Bin1, bridging integrator 1 ( MGI:108092)
Sequence:	sense strand is shown >T36049 TACCTAGGCCAGTTCCCTGATATCAAGTCGCGCATTGCCAAGCGGGGGCGGAAGCTGGTGGACTATGACA GTGCCCGGCACCACTATGAGTCTCTTCAAACCGCCAAAAAGAAGGATGAAGCCAAAATTGCCAAGCCTGT CTCGCTGCTTGAGAAAGCCGCCCCCCAGTGGTGCCAAGGCAAACTACAGGCTCATCTTGTAGCTCAAACT AACCTGCTCCGAAATCAGGCAGAAGAGGAGCTCATCAAAGCCCAGAAGGTGTTCGAGGAGATGAACGTGG ATCTGCAGGAGGAGCTGCCATCCCTGTGGAACAGCCGTGTAGGTTTCTATGTCAACACGTTCCAGAGCAT CGCGGGTCTGGAGGAAAACTTCCATAAAGAGATGAGTAAGCTCAATCAGAACCTCAATGATGTCCTGGTC AGCCTAGAGAAGCAGCACGGGAGCAACACCTTCACAGTCAAGGCCCAACCCAGTGACAATGCCCCTGAGA AAGGGAACAAGAGCCCGTCACCTCCTCCAGATGGCTCCCCTGCTGCTACCCCTGAGATCAGAGTGAACCA TGAGCCAGAGCCGGCCAGTGGGGCCTCACCCGGGGCTACCATCCCCAAGTCCCCATCTCAGCTCCGGAAA GGCCCACCTGTCCCTCCGCCTCCCAAACACACCCCATCCAAGGAGATGAAGCAGGAGCAGATTCTCAGCC TTTTTGATGACGCATTTGTCCCTGAGATCAGCGT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 98909. Forward Primer - name:098909_F_cDNA_Bin1, sequence:TACCTAGGCCAGTTCCCTGATA; Reverse Primer - name:098909_N_SP6_cDNA_Bin1, sequence:ACGCTGATCTCAGGGACAAAT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14970 same embryo
	EMAGE:14971 same embryo
	EMAGE:14968 same embryo
	EurExpress:euxassay_008004 same experiment
	MGI:4823475 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source