EMAGE mouse gene expression spatial database

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EMAGE:14982

Dync1h1 dynein cytoplasmic 1 heavy chain 1 ( MGI:103147)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008019. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008019_01	euxassay_008019_02	euxassay_008019_03	euxassay_008019_04

euxassay_008019_05	euxassay_008019_06	euxassay_008019_07	euxassay_008019_08	euxassay_008019_09

euxassay_008019_10	euxassay_008019_11	euxassay_008019_12	euxassay_008019_13	euxassay_008019_14

euxassay_008019_15	euxassay_008019_16	euxassay_008019_17	euxassay_008019_18	euxassay_008019_19

euxassay_008019_20	euxassay_008019_21	euxassay_008019_22	euxassay_008019_23	euxassay_008019_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14982_wholemount_strong.wlz

14982_wholemount_moderate.wlz

14982_wholemount_weak.wlz

14982_wholemount_possible.wlz

14982_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14982_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

forebrain

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

hindbrain

moderate

homogeneous

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

midbrain

moderate

homogeneous

moderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18

facial vii ganglion

strong

regional

strong expression: see section 04 05 19 20

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 06 17 moderate expression: see section 16

trigeminal v ganglion

strong

regional

strong expression: see section 03 04 05 06 07 08 09 17 18 19 20 21 22

vagus x ganglion

strong

regional

strong expression: see section 07

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 06 07 17 moderate expression: see section 18

trigeminal v nerve

strong

regional

strong expression: see section 17 moderate expression: see section 10

spinal cord

moderate

homogeneous

moderate expression: see section 06 07 08 09 10 11 12 13

dorsal root ganglion

strong

regional

strong expression: see section 05 06 07 08 09 10 13 14 15

neural retina

moderate

regional

moderate expression: see section 02 03 04 05 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T36296
Entity Detected:	Dync1h1, dynein cytoplasmic 1 heavy chain 1 ( MGI:103147)
Sequence:	sense strand is shown >T36296 ACATTTAGCAGCATCCCTGTCTCTAGGATATGCAAGTCTCCAAATGAGCGCGCCCGCTTGTATTTCCTGC TGGCTTGGTTCCATGCCATCATCCAAGAGCGCCTGCGCTATGCTCCACTGGGGTGGTCAAAGAAGTACGA ATTTGGAGAATCTGACCTTCGATCAGCTTGTGACACGGTGGACACGTGGCTGGACGACACAGCCAAGGGC CGGCAGAACATCTCACCGGATAAGATCCCATGGTCTGCCCTGAAGACCCTGATGGCGCAGTCCATCTATG GTGGGCGGGTGGACAATGAGTTCGACCAGCGTCTGCTCAACACCTTCCTGGAGCGGCTGTTCACCACCCG CAGCTTCGACAGTGAATTCAAGCTGGCGTGCAAGGTTGACGGGCACAAGGACATCCAGATGCCTGATGGT ATCAGGCGAGAGGAGTTTGTGCAGTGGGTGGAGCTGCTGCCTGACGCCCAGACACCCTCATGGCTGGGCC TCCCCAACAATGCTGAAAGGGTCCTGCTTACCACCCAAGGCGTGGACATGATCAGCAAGATGCTGAAGAT GCAGATGCTGGAGGACGAGGATGACCTGGCCTACGCAGAGACGGAGAAGAAGGCAAGGACGGACTCCACC TCCGATGGCCGTCCAGCCTGGATGAGGACACTGCACACCACGGCGTCCAACTGGCTGCACCTCATCCCAC AGACACTGAGCCCCCTGAAGCGCACCGTGGAGAACATCAAGGATCCT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 91739. Forward Primer - name:091739_F_cDNA_Dnchc1, sequence:ACATTTAGCAGCATCCCTGTCT; Reverse Primer - name:091739_N_SP6_cDNA_Dnchc1, sequence:AGGATCCTTGATGTTCTCCAC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14979 same embryo
	EMAGE:14980 same embryo
	EMAGE:14978 same embryo
	EMAGE:14983 same embryo
	EMAGE:14981 same embryo
	EurExpress:euxassay_008019 same experiment
	MGI:4824427 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source