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EMAGE:15139

Arhgap20 Rho GTPase activating protein 20 ( MGI:2445175)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:15139 EMAGE:15139 EMAGE:15139 EMAGE:15139 EMAGE:15139
"Pseudo-wholemount" of euxassay_010398. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_010398_01 euxassay_010398_02 euxassay_010398_03 euxassay_010398_04
EMAGE:15139 EMAGE:15139 EMAGE:15139 EMAGE:15139 EMAGE:15139
euxassay_010398_05 euxassay_010398_06 euxassay_010398_07 euxassay_010398_08 euxassay_010398_09
EMAGE:15139 EMAGE:15139 EMAGE:15139 EMAGE:15139 EMAGE:15139
euxassay_010398_10 euxassay_010398_11 euxassay_010398_12 euxassay_010398_13 euxassay_010398_14
EMAGE:15139 EMAGE:15139 EMAGE:15139 EMAGE:15139 EMAGE:15139
euxassay_010398_15 euxassay_010398_16 euxassay_010398_17 euxassay_010398_18 euxassay_010398_19
EMAGE:15139 EMAGE:15139 EMAGE:15139 EMAGE:15139
euxassay_010398_20 euxassay_010398_21 euxassay_010398_22 euxassay_010398_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:15139Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
15139_wholemount_strong.wlz
15139_wholemount_moderate.wlz
15139_wholemount_weak.wlz
15139_wholemount_possible.wlz
15139_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:15139_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
mesenchyme
weak weak
regionalweak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T37564
Entity Detected:Arhgap20, Rho GTPase activating protein 20 ( MGI:2445175)
Sequence:sense strand is shown

>T37564
CCCTACATAAAGGAACCTGCTGATAGCATGCATCTCATAGCGCAGAAGAATAGCCACATAATAATTAGGG
AAATTACTCTCGAGGTCAGTTTTCCCTGGCTGACTAATCTCAGGGGCAAGACTTCAGAGAAACAAGAGAT
GCTGGAGTTGGTAGCAGCCATGCAATGGTATTGTGCTGTGATCAGTAAGTGTGTGCAGAGATGTACCCAG
ATCAGGAAGCTGGGTCCTGCAGTACATACAGTATATTACAGAAAGTGGCCTCTCTTTCTTAGACCTAATG
GCCAGTTGCCATGTGACACTTGTGTGCACAGATATACCCAGATCAGGAAGCTGGGTCCTGCAATACATAC
AAGATATTACAGAAAGTGGCCCCTCTAACTTAGACCTAATGGCCAGTTACCATATGACACTTCTCTATAT
AAGTCATACTTTTCTCATTTTGAAAAATGGCAGTTTCCTGCAACAGCTCTCCCTTCTTGGTTCCACAAGC
AGTATTTATCAATATGGCGTCTCTGTGGAATGTTTCACTGTAGCCCTTCCTGTTAGATTACAGTCAGCCA
AGAGGAGACAAGGGACAGCTGTTAATTATTCTTTCATTCTACAGATTGTTCCCGATTTGAGATTGGCAGA
CTGTAAGATTATTGAGGAGAACACAATGACATTGCTCTTGGGTGGTCTCTGATGGCCACTGCCTTTGGGT
TTTCTTGCTGAAGTTCTCCACTTTTTCCAAATGATTTATAGTTTATAAATATTCAAGTTTAGACCCTGGC
AGTTTATCATAGACCTTTGTCCTCTCTCTCTACCTTCAAGGCTACATCAGTATGTCTTCCTTCCACAGCC
AGAATGTAATGGAAGGA
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 71552. Forward Primer - name:071552_F_cDNA_Arhgap20, sequence:CCCTACATAAAGGAACCTGCTG; Reverse Primer - name:071552_N_SP6_cDNA_Arhgap20, sequence:TCCTTCCATTACATTCTGGCTG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:15137 same embryo
 EMAGE:15140 same embryo
 EMAGE:15142 same embryo
 EMAGE:15138 same embryo
 EMAGE:15141 same embryo
 EurExpress:euxassay_010398 same experiment
 MGI:4823217 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS