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EMAGE:15345

Cd200 CD200 antigen ( MGI:1196990)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:15345 EMAGE:15345 EMAGE:15345 EMAGE:15345 EMAGE:15345
"Pseudo-wholemount" of euxassay_010522. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_010522_01 euxassay_010522_02 euxassay_010522_03 euxassay_010522_04
EMAGE:15345 EMAGE:15345 EMAGE:15345 EMAGE:15345 EMAGE:15345
euxassay_010522_05 euxassay_010522_06 euxassay_010522_07 euxassay_010522_08 euxassay_010522_09
EMAGE:15345 EMAGE:15345 EMAGE:15345 EMAGE:15345 EMAGE:15345
euxassay_010522_10 euxassay_010522_11 euxassay_010522_12 euxassay_010522_13 euxassay_010522_14
EMAGE:15345 EMAGE:15345 EMAGE:15345 EMAGE:15345 EMAGE:15345
euxassay_010522_15 euxassay_010522_16 euxassay_010522_17 euxassay_010522_18 euxassay_010522_19
EMAGE:15345 EMAGE:15345 EMAGE:15345 EMAGE:15345 EMAGE:15345
euxassay_010522_20 euxassay_010522_21 euxassay_010522_22 euxassay_010522_23 euxassay_010522_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:15345Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
15345_wholemount_strong.wlz
15345_wholemount_moderate.wlz
15345_wholemount_weak.wlz
15345_wholemount_possible.wlz
15345_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:15345_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
rib
strong strong
regionalstrong expression: see section 02 03 04 05 06 07 08 17 18 19 20 21 22 23
radius
strong strong
regionalstrong expression: see section 01
ulna
strong strong
regionalstrong expression: see section 01
humerus
strong strong
regionalstrong expression: see section 01 02 03 22 23 24
fibula
strong strong
regionalstrong expression: see section 01 02 24 moderate expression: see section 23
tibia
strong strong
regionalstrong expression: see section 01 02 24 moderate expression: see section 23
femur
strong strong
regionalstrong expression: see section 03 04 05 20 21 22
brain
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
vibrissa
weak weak
regionalweak expression: see section 06 07 08 09 22 23
facial vii ganglion
moderate moderate
regionalmoderate expression: see section 04 05 06 07 19 20 21
glossopharyngeal ix ganglion
moderate moderate
regionalmoderate expression: see section 06 07 17 18
trigeminal v ganglion
moderate moderate
regionalmoderate expression: see section 03 04 05 06 07 08 09 17 18 19 20 21 22
vagus x ganglion
moderate moderate
regionalmoderate expression: see section 07 08 17
vestibulocochlear viii ganglion
moderate moderate
regionalmoderate expression: see section 05 06 07 08 17 18 19
trigeminal v nerve
moderate moderate
regionalmoderate expression: see section 10 17
spinal cord
moderate moderate
regionalmoderate expression: see section 08 09 10 11 12 13 14 15
cervico-thoracic ganglion
weak weak
regionalweak expression: see section 10 16
cervical ganglion
moderate moderate
regionalmoderate expression: see section 17 weak expression: see section 09 16
thoracic ganglion
weak weak
regionalweak expression: see section 11 14
dorsal root ganglion
moderate moderate
regionalmoderate expression: see section 06 07 08 09 10 11 14 15 16 17
neural retina
moderate moderate
regionalmoderate expression: see section 02 03 04 23 24
anterior naris
moderate moderate
regionalmoderate expression: see section 12
viscerocranium
moderate moderate
regionalExpression in the turbinate bone.
aorta
moderate moderate
regionalmoderate expression: see section 10 11 12 13 14 15
mandible
strong strong
regionalstrong expression: see section 03 04 05 06 07 08 09 10 11 12 13 16 17 18 19 20 21 22 23
maxilla
strong strong
regionalstrong expression: see section 04 05 06 07 08 09 10 11 12 13 17 18 19 20 21 22 23
basisphenoid bone
moderate moderate
regionalmoderate expression: see section 11 12
orbito-sphenoid
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 21 22 23 24
clavicle
strong strong
regionalstrong expression: see section 04 05 06 07 08 09 16 17 18 19 20 21
scapula
strong strong
regionalstrong expression: see section 02 03 22 23
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T30567
Entity Detected:Cd200, CD200 antigen ( MGI:1196990)
Sequence:sense strand is shown

>T30567
AAGAAAGGCGCTGCACACAACTGCATCCTTACGATGTTCTCTAAAAACATCCCAGGAACCCTTGATTGTG
ACATGGCAGAAAAAGAAAGCCGTGAGCCCAGAAAACATGGTCACCTACAGCAAAACCCATGGGGTTGTAA
TCCAGCCTGCCTACAAAGACAGGATAAATGTCACAGAGCTGGGACTCTGGAACTCAAGCATCACCTTCTG
GAACACAACATTGGAAGATGAGGGCTGCTACATGTGTCTCTTCAACACGTTTGGTTCTCAGAAGGTCTCA
GGAACAGCTTGCCTTACCCTCTATGTACAGCCCATAGTACACCTTCACTACAACTATTTTGAAGACCACC
TAAACATCACTTGCTCTGCGACTGCCCGTCCAGCCCCTGCCATCTCCTGGAAGGGTACTGGGACAGGAAT
TGAGAATAGTACCGAGAGTCACTTCCATTCAAATGGGACTACATCTGTCACCAGCATCCTCCGGGTCAAA
GACCCCAAAACTCAAGTTGGGAAGGAAGTGATCTGCCAGGTTTTATACCTGGGGAATGTGATTGACTACA
AGCAGAGTCTGGACAAAGGATTTTGGTTTTCAGTTCCACTGTTGCTAAGCATTGTTTCTCTGGTAATTCT
TCTGATCTTGATCTCCATCTTACTATACTGGAAACGTCACCGAAATCAGGAGCGGGGTGAATCATCACAG
GGGATGCAAAGAATGAAATAAGAGCTCTAAAGAAATTATACAGAACCCTGAACGTGTTTCCCTGGTCTAC
TTGAATCTGATGTGAAAGAAAAGCAGGAGGGAAAAGGCCA
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:5707683), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 59014. Forward Primer - name:059014_F_IRAV112_d08_Cd200, sequence:AAGAAAGGCGCTGCACAC; Reverse Primer - name:059014_R_SP6_IRAV112_d08_Cd200, sequence:ATGGCCTTTTCCCTCCTG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:15344 same embryo
 EMAGE:15348 same embryo
 EMAGE:15346 same embryo
 EMAGE:15347 same embryo
 EMAGE:15343 same embryo
 EurExpress:euxassay_010522 same experiment
 MGI:4823720 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS