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EMAGE:15789

Slc4a5 solute carrier family 4, sodium bicarbonate cotransporter, member 5 ( MGI:2443220)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:15789 EMAGE:15789 EMAGE:15789 EMAGE:15789 EMAGE:15789
"Pseudo-wholemount" of euxassay_013237. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_013237_01 euxassay_013237_02 euxassay_013237_03 euxassay_013237_04
EMAGE:15789 EMAGE:15789 EMAGE:15789 EMAGE:15789 EMAGE:15789
euxassay_013237_05 euxassay_013237_06 euxassay_013237_07 euxassay_013237_08 euxassay_013237_09
EMAGE:15789 EMAGE:15789 EMAGE:15789 EMAGE:15789 EMAGE:15789
euxassay_013237_10 euxassay_013237_11 euxassay_013237_12 euxassay_013237_13 euxassay_013237_14
EMAGE:15789 EMAGE:15789 EMAGE:15789 EMAGE:15789 EMAGE:15789
euxassay_013237_15 euxassay_013237_16 euxassay_013237_17 euxassay_013237_18 euxassay_013237_19
EMAGE:15789 EMAGE:15789 EMAGE:15789 EMAGE:15789
euxassay_013237_20 euxassay_013237_21 euxassay_013237_22 euxassay_013237_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:15789Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
15789_wholemount_strong.wlz
15789_wholemount_moderate.wlz
15789_wholemount_weak.wlz
15789_wholemount_possible.wlz
15789_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:15789_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
lateral ventricle choroid plexus
strong strong
regionalstrong expression: see section 07 moderate expression: see section 08 09 10 11 12 13 18 19 20 weak expression: see section 16 17
metencephalon part of 4th ventricle choroid plexus
strong strong
regionalstrong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T39849
Entity Detected:Slc4a5, solute carrier family 4, sodium bicarbonate cotransporter, member 5 ( MGI:2443220)
Sequence:sense strand is shown

>T39849
CCCTCATGGTGGATGATCTATTCAGCGATGTGGCTTATAAAGCTCGGAACCGAGAAGATCTGATCGCAGG
GATTGATGAATTTCTAGACGAGGTCATTGTCCTTCCCCCTGGAGAGTGGGACCCAAACATCAGAATTGAG
CCACCCAAGAAAGTTCCCTCAGCTGACAAGAGGAAATCTGTGTTCTCTCTGGCAGAGCCGGGCCAGATGA
ATGGCTCTGTGGGCGGAGGCGGAGCCTCGGCTGGAGGAGGCGGCAGTGGCGGAGGGGCTGGAGGCAGCGG
GGCTGGCGGAGTGGGCAGTGGCGACGAAGCTGAGATGCCAGCTATGCACGAAATCGGGGAGGAGCTGATC
TGGACCGGAAGGTTCTTTGGAGGACTGTGTCTGGATGTCAAGAGGAAGCTGCCCTGGTTCCCAAGCGACT
TCTATGATGGCTTCCACCTCCAGTCCATCTCTGCTGTTCTGTTCATCTACCTCGGCTGTATTACCAACGC
GATCACCTTTGGTGGGCTTCTGGGGGATGCCACAGACAACTACCAGGGAGTAATGGAGAGCTTCCTGGGC
ACTGCCATGGCTGGCAGCTTGTTCTGCCTCTTCTC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 108022. Forward Primer - name:108022_F_cDNA_LOC381788, sequence:CCCTCATGGTGGATGATCTATT; Reverse Primer - name:108022_N_SP6_cDNA_LOC381788, sequence:GAGAAGAGGCAGAACAAGCTG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:15793 same embryo
 EMAGE:15788 same embryo
 EMAGE:15790 same embryo
 EMAGE:15791 same embryo
 EMAGE:15792 same embryo
 EurExpress:euxassay_013237 same experiment
 MGI:4828246 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS