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EMAGE:15847

Dok6 docking protein 6 ( MGI:3639495)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:15847 EMAGE:15847 EMAGE:15847 EMAGE:15847 EMAGE:15847
"Pseudo-wholemount" of euxassay_013254. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_013254_01 euxassay_013254_02 euxassay_013254_03 euxassay_013254_04
EMAGE:15847 EMAGE:15847 EMAGE:15847 EMAGE:15847 EMAGE:15847
euxassay_013254_05 euxassay_013254_06 euxassay_013254_07 euxassay_013254_08 euxassay_013254_09
EMAGE:15847 EMAGE:15847 EMAGE:15847 EMAGE:15847 EMAGE:15847
euxassay_013254_10 euxassay_013254_11 euxassay_013254_12 euxassay_013254_13 euxassay_013254_14
EMAGE:15847 EMAGE:15847 EMAGE:15847 EMAGE:15847 EMAGE:15847
euxassay_013254_15 euxassay_013254_16 euxassay_013254_17 euxassay_013254_18 euxassay_013254_19
EMAGE:15847
euxassay_013254_20

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:15847Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
15847_wholemount_strong.wlz
15847_wholemount_moderate.wlz
15847_wholemount_weak.wlz
15847_wholemount_possible.wlz
15847_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:15847_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
forebrain
weak weak
regionalweak expression: see section 01 02 03 04 05 07 08 09 10 11 12 13 14 15 16 17 18 19
hindbrain
weak weak
regionalweak expression: see section 03 04 05 07 08 09 10 11 12 13 14
midbrain
weak weak
regionalweak expression: see section 03 04 05 07 08 09 10 11 12
facial vii ganglion
weak weak
regionalweak expression: see section 03 15 16
glossopharyngeal ix ganglion
weak weak
regionalweak expression: see section 04 05 13 14
trigeminal v ganglion
weak weak
regionalweak expression: see section 02 03 04 05 13 14 15 16 17 18
spinal cord
weak weak
regionalweak expression: see section 07 08 09 10
dorsal root ganglion
weak weak
regionalweak expression: see section 04 05 06 11 12
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T39865
Entity Detected:Dok6, docking protein 6 ( MGI:3639495)
Sequence:sense strand is shown

>T39865
CACCCAACCTGGATATTTATGGTGAATGCACAATGCAGATCACTCATGAGAATATCTATCTCTGGGATAT
CCACAATGCGAAGGTCAAGCTAGTGATGTGGCCTCTTAGCTCGCTGAGGAGATATGGTCGGGATTCAACT
TGGTTCACCTTCGAGTCAGGAAGAATGTGTGACACAGGAGAAGGACTATTCACTTTTCAAACAAGGGAAG
GAGAAATGATCTATCAGAAGGTCCACTCTGCAACACTGGCTATAGCTGAGCAGCACGAAAGATTAATGCT
AGAAATGGAGCAGAAGGCCCGGCTGCAGACAAGCTTGACTGAGCCGATGACGTTATCGAAATCCATCTCT
CTTCCTCGGAGTGCATACTGGCATCACATTACACGTCAGAAC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 111537. Forward Primer - name:111537_F_cDNA_LOC383319, sequence:CACCCAACCTGGATATTTATGG; Reverse Primer - name:111537_N_SP6_cDNA_LOC383319, sequence:GTTCTGACGTGTAATGTGATGC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:15842 same embryo
 EMAGE:15846 same embryo
 EMAGE:15843 same embryo
 EMAGE:15845 same embryo
 EMAGE:15844 same embryo
 EurExpress:euxassay_013254 same experiment
 MGI:4824374 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS