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EMAGE:15950

LOC433868 (LOC433868)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:15950 EMAGE:15950 EMAGE:15950 EMAGE:15950 EMAGE:15950
"Pseudo-wholemount" of euxassay_013384. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_013384_01 euxassay_013384_02 euxassay_013384_03 euxassay_013384_04
EMAGE:15950 EMAGE:15950 EMAGE:15950 EMAGE:15950 EMAGE:15950
euxassay_013384_05 euxassay_013384_06 euxassay_013384_07 euxassay_013384_08 euxassay_013384_09
EMAGE:15950 EMAGE:15950 EMAGE:15950 EMAGE:15950 EMAGE:15950
euxassay_013384_10 euxassay_013384_11 euxassay_013384_12 euxassay_013384_13 euxassay_013384_14
EMAGE:15950 EMAGE:15950 EMAGE:15950 EMAGE:15950 EMAGE:15950
euxassay_013384_15 euxassay_013384_16 euxassay_013384_17 euxassay_013384_18 euxassay_013384_19
EMAGE:15950 EMAGE:15950
euxassay_013384_20 euxassay_013384_21

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:15950Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
15950_wholemount_strong.wlz
15950_wholemount_moderate.wlz
15950_wholemount_weak.wlz
15950_wholemount_possible.wlz
15950_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:15950_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
rib
moderate moderate
regionalmoderate expression: see section 01 02 03 04 07 14 15 16 17 18 19
humerus
moderate moderate
regionalmoderate expression: see section 03 04 18 19
lower lip
strong strong
regionalstrong expression: see section 07
lower jaw mesenchyme
strong strong
regionalstrong expression: see section 07 15
upper lip
strong strong
regionalstrong expression: see section 09 10 11 12 13 14 15
upper jaw mesenchyme
strong strong
regionalstrong expression: see section 07 15
axial skeleton
strong strong
regionalstrong expression: see section 07 08 09 10 11
clavicle
moderate moderate
regionalmoderate expression: see section 06 07 09 10 11 14 15
axial skeleton tail region
strong strong
regionalstrong expression: see section 11 12
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T40087
Entity Detected:LOC433868, (LOC433868)
Sequence:sense strand is shown

>T40087
TATATGTAGCCTGGGACGCTTTCATAAACACTGATGACGTGTTTGTGCAAAGCAAGCAATTTGAGGAGAA
ACGCCTGGGACGTCGGAAAGAAGGAGTGATCGATTACAAAGAACGTTCCTTCAATCAGGTGAAGGCTCTC
CTCAGAAGATTTCCTGTCTCTGC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 151276. Forward Primer - name:151276_F_cDNA_LOC435768, sequence:TATATGTAGCCTGGGACGCTTT; Reverse Primer - name:151276_N_SP6_cDNA_LOC435768, sequence:GCAGAGACAGGAAATCTTCTGAG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:15949 same embryo
 EMAGE:15948 same embryo
 EMAGE:15952 same embryo
 EMAGE:15953 same embryo
 EMAGE:15951 same embryo
 EurExpress:euxassay_013384 same experiment
 MGI:4825544 same experiment
  Allen Brain Atlas same gene
  BioGPS same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS