EMAGE mouse gene expression spatial database

EMAGE:16119

Fgf17 fibroblast growth factor 17 ( MGI:1202401)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_013537. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_013537_01	euxassay_013537_02	euxassay_013537_03	euxassay_013537_04

euxassay_013537_05	euxassay_013537_06	euxassay_013537_07	euxassay_013537_08	euxassay_013537_09

euxassay_013537_10	euxassay_013537_11	euxassay_013537_12	euxassay_013537_13	euxassay_013537_14

euxassay_013537_15	euxassay_013537_16	euxassay_013537_17	euxassay_013537_18	euxassay_013537_19

euxassay_013537_20	euxassay_013537_21	euxassay_013537_22	euxassay_013537_23

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Expression Pattern Description

Spatial Annotation:

Annotation colour key:

wholemount mapping

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Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

telencephalon ventricular layer

moderate

regional

moderate expression: see section 10 11 12

rest of cerebellum ventricular layer

moderate

regional

moderate expression: see section 10 11 12 13 14 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T45074
Entity Detected:	Fgf17, fibroblast growth factor 17 ( MGI:1202401)
Sequence:	sense strand is shown >T45074 CGGGTCAGGAATAAATCAAGAGAGATCGTAGGGGCCAGCCTCCTGATAGAGGACGCTCTCGCAGCTCCAA TCCTTTGCGCCCGGGGACCTCTGCCCGAAGCCAGTAGCCCAAGAGAGCGAGTACGCCAATC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from genomic DNA prepared from tail-tips of two wild-type C57BL/6J mice), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 190289. Forward Primer - name:190289_F_exon_Fgf17, sequence:CGGGTCAGGAATAAATCAAGAG; Reverse Primer - name:190289_N_SP6_exon_Fgf17, sequence:GATTGGCGTACTCGCTCTCTT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:16120 same embryo
	EMAGE:16116 same embryo
	EMAGE:16118 same embryo
	EMAGE:16115 same embryo
	EMAGE:16117 same embryo
	EurExpress:euxassay_013537 same experiment
	MGI:4824842 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source