EMAGE mouse gene expression spatial database

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EMAGE:16746

Mir539 microRNA 539 ( MGI:3619427)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_019297. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_019297_01	euxassay_019297_02	euxassay_019297_03	euxassay_019297_04

euxassay_019297_05	euxassay_019297_06	euxassay_019297_07	euxassay_019297_08	euxassay_019297_09

euxassay_019297_10	euxassay_019297_11	euxassay_019297_12	euxassay_019297_13	euxassay_019297_14

euxassay_019297_15	euxassay_019297_16	euxassay_019297_17	euxassay_019297_18	euxassay_019297_19

euxassay_019297_20	euxassay_019297_21	euxassay_019297_22	euxassay_019297_23	euxassay_019297_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

16746_wholemount_strong.wlz

16746_wholemount_moderate.wlz

16746_wholemount_weak.wlz

16746_wholemount_possible.wlz

16746_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:16746_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

humerus

moderate

regional

moderate expression: see section 01 02 weak expression: see section 20 21 22 24

femur

moderate

regional

moderate expression: see section 02 03 weak expression: see section 17 18 21

scapula

weak

regional

weak expression: see section 01 02

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T70082
Entity Detected:	Mir539, microRNA 539 ( MGI:3619427)
Sequence:	sense strand is shown >T70082 GGAGAAATTATCCTTGGTGTGT
Notes:	The probe used by the EURExpress consortium to detect the presence of miRNA mmu-miR-539 was custom designed and ordered from Sigma-Exiqon by the EURExpress partner in Geneva and then distributed to the ISH generating units within the consortium.
Chemistry:	LNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:16747 same embryo
	EMAGE:16744 same embryo
	EMAGE:16743 same embryo
	EMAGE:16745 same embryo
	EurExpress:euxassay_019297 same experiment
	MGI:4826330 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source