EMAGE mouse gene expression spatial database

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EMAGE:17118

Dnahc1 dynein, axonemal, heavy chain 1 ( MGI:107721)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001508. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001508_01	euxassay_001508_02	euxassay_001508_03	euxassay_001508_04

euxassay_001508_05	euxassay_001508_06	euxassay_001508_07	euxassay_001508_08	euxassay_001508_09

euxassay_001508_10	euxassay_001508_11	euxassay_001508_12	euxassay_001508_13	euxassay_001508_14

euxassay_001508_15	euxassay_001508_16	euxassay_001508_17	euxassay_001508_18	euxassay_001508_19

euxassay_001508_20	euxassay_001508_21	euxassay_001508_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17118_wholemount_strong.wlz

17118_wholemount_moderate.wlz

17118_wholemount_weak.wlz

17118_wholemount_possible.wlz

17118_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17118_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

lateral ventricle choroid plexus

moderate

regional

moderate expression: see section 06 07 08 09 12 15 16 17 18

cerebellum

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 18 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4501
Entity Detected:	Dnahc1, dynein, axonemal, heavy chain 1 ( MGI:107721)
Sequence:	sense strand is shown >T4501 CGGACGCGTGGGCGGACGCGTGGGTCGAAGCACTGAAAACTTTGCTGTTTAGGGGAGCCGGAGCTCTGGC TGGGACGCCTCTCTGAAGAACAGCATGGAGGAGTGTAATAAAGAAGGACCAAGCAGCAGCTCTCAGGGCC CAGGATACTGTCCGGTGAAGGTTCCTGAAAGCCATGACCTGGAGAAGATATTACAAGAATCAAATTATCA TCCAGAAAGAAATCCCCTAAATCCTGACCCCAAGACCCCGCCTTTACCCCTCACAGACCTAAGACAGCCC AGGAAGTCGCCTTTGACAGGCACTGATAAGAAGTACCCCCTGATGAAGCAGCGCGGATTCTATTCTGACA TCCTCAGCCCTGGAACCTTAGACAAGCTTGGGAATGTGTGCTGTGGCCCCTACATGTCCCAGAATTTGAT TCGCCAAGCTGACCTCGACAAGTTCACCCCAAAAGTTGACTCATTCGTCATTCCTGAGGACTTTCAGGAG CGTGTGGAGCAGCAGATCATTGGTGCTACCACGCGGCTGCTCACCCAGACTGACTTCCCGCTACAGTCCT ATGAGCCCAAAGTGCAGGTCCCATTCCAGGTGCTGCCTGGCCAGTGCCCTCGGAAGATTGAGATCGAGAG GAGGAAGCAGCAATACTTGCGCCTGGACATTGAGCAGCTGTTGACCAGCGAGGGCATCGACTCTAACAAG CTCATGCCCAGACATCCAGACCTGCAGCACCCACAAACCATCGAACAGGGCCGTGATCCACTCTTCCCCA TATATCTCCCACTGAAGGTGTTTGATAATGAAGAGTTTGATTGCCGGACTCCCACAGAGTGGCTCAACAT GGGCTTGGAGCCAGGCTCACAAAACAGGAAGCCCGTCCCTGGAAAAGCCCTTCTTCCCACAGACGACGAT CTGGGGCACGAGGACCCCAAGAATCAGGAACTGGACTATAGATGGTGTGAAGTTGGCGTCCTTGACTACG ATGAAGAAAAAAAACTGTATTTGGTGCAGAAAACTGACAAGAGAGGCTTGGTGCGCGATGAGATGGGGAT GCCCATCCTGAATGGAGGGATCACCCCTGCGGGAAGGCCACCACTCCTGGCCACCCAGTACTGGGTCCCG AGGATCCAGCTCCTCTTCTGTGCTGAGGATCCACGTGTGTTCACACAGCGCGTGGTCCAGGCCAACGCTC TGCGCAAGTACACAGAGGCGCTGTTGATGTACAACCTGTATGTGGATTGCATGCCCACCGAGGGCCGGCG GGTCATCAATGAGCAGAGCTTGAGCAAGATAAAGCAGTGGGCTTTGAGCACACCGCGCATGCGCAAGGGG CAGAGCGTCCTGGAGCATCTTAGCTGCCTGGCCAGAGAAGTGAACCTGGACTACGAACGTAGCATGAACA AGATCAACTTTGACCAAATTGTCTCTTCTAACCCAGAGACATTCTCCTATGTGACTCTTCCTGAAAAAGA GGAAGAGAAGGTACCCAATCAAGGTGAGAGCCACTGGACCCTGGCTGCCCACCTGGCCAGCTCACCCCAC CTCCGCCTCTCTTGATTTTGTTCAGTCAGTAAAGGTCTAAAAGTCTCAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4952398 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4952398 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17120 same embryo
	EMAGE:17117 same embryo
	EMAGE:17119 same embryo
	EurExpress:euxassay_001508 same experiment
	MGI:4824337 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source