EMAGE mouse gene expression spatial database

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EMAGE:17164

Slc25a21 solute carrier family 25 (mitochondrial oxodicarboxylate carrier), member 21 ( MGI:2445059)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_001565_01	euxassay_001565_02	euxassay_001565_03	euxassay_001565_04	euxassay_001565_05

euxassay_001565_06	euxassay_001565_07	euxassay_001565_08	euxassay_001565_09	euxassay_001565_10

euxassay_001565_11	euxassay_001565_12	euxassay_001565_13	euxassay_001565_14	euxassay_001565_15

euxassay_001565_16	euxassay_001565_17	euxassay_001565_18	euxassay_001565_19	euxassay_001565_20

euxassay_001565_21	euxassay_001565_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17164_wholemount_strong.wlz

17164_wholemount_moderate.wlz

17164_wholemount_weak.wlz

17164_wholemount_possible.wlz

17164_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17164_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

liver lobe

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4763
Entity Detected:	Slc25a21, solute carrier family 25 (mitochondrial oxodicarboxylate carrier), member 21 ( MGI:2445059)
Sequence:	sense strand is shown >T4763 GATAAGCAGCCAGCTAAACAAACTCCGCAGTAGGTCTCGATAGGCAAATTCATAAGCCCCTATCTTGAAG CTCGAGCCTGACCCACCGAGCTAGGTCAAGTCTACCCAGCCCCCAGCTGTCAGTGTTCCCCCTCCGGTGC CCCGGTGGAAAGGGCAGCCTTAGGCAGCGCTGGGCAAGTTCGGACCCCCTCCGCCAACCTCGCGGCGACT CGGTGCCCGAACCGCCGCCTTTAGCTGGATCGCAGCCACACCGGGAAGAGTTCAGCCCCAGGACCGACGG GGCCCGGTCTCCATCGGCGGCGGCCTTCAGAGGAGCTCGCTCGGGCAACTTGGTCACCCGGTCCCCTGGT CGCGAAGATGTCGGCCTCCAACGTCAGCTTACTGCACGAGACCTCCAGGCAGGTCGCGGCCGGAGGGTCT GCAGGTCTTGTAGAAATTTGCCTGATGCATCCTCTCGATGTGGTGAAAACCAGGTTCCAGGTGCAGAGAA GTGTAACAGACCCACAAAGTTATAGAACTGTAAGAGGCAGCTTCCAGATGATCTTCCGAACAGAGGGGTT GTTTGGCTTTTACAAGGGAATTATCCCACCAATTTTAGCTGAAACACCAAAAAGAGCAGTCAAGTTTTCT ACCTTTGAGCTGTACAAGAAATTTCTGGGATATATGTCACTATCACCAGGGCTGACATTTCTCATCGCTG GCTTAGGATCGGGACTAACAGAAGCTGTCGTGGTTAACCCTTTCGAGGTGGTAAAAGTTGGCTTGCAAGT CAATCGGAACTTGTTCAAAGAGCAACCATCTACTTTTGCTTATGCAAGACAAATCATTAAGAAGGAAGGC TTGGGGTTCCAGGGCCTCAACAAAGGATTGACAGCAACTCTGGGACGTCACGGAATTTTCAACATGGTTT ATTTTGGCTTCTACCACAACGTCAAAAATATTATTCCATCCAGTAAGGATCCAACCTTGGAGTTTTTGAG AAAATTTGGGATTGGTTTTGTCTCAGGGACAATGGGCTCAGTGTTTAACATCCCCTTTGATGTTGCAAAG AGTCGGATCCAAGGGCCCCAGCCAGTTCCTGGAGAGATCAAGTACCGAAGCTGCTTCAAAACAATGGAGA TGATCTACCGGGAAGAAGGGATTTTAGCCTTATACAAAGGCCTGGTCCCTAAGGTGATGAGACTTGGACC AGGTGGTGGGGTGATGTTGCTGGTGTATGAATACACCTATGCATGGCTGCAGGAAAACTGGTGATGGACT GAGAAGCGATTTCCTCTTGACAAAACAGCTCTGCATCCCCAGGAAAAGAAGATAAGCTAGACACACCCGT TAAGAGGGGAAGACAACGTGCTCAGGAACAAAGCCTGTTCCAGTGTTTTATAAATTTTTAGATAAATTGA CATGGTAGTTTGGGCCATATACATAACACAAGTCAAAATTTAAATATATTTTATAAGGAATTACTTTTCC ATACGTTATATTTTAAAAGAGAAGTAAGAAAAATGTAAATACACCAAAACAGCCTTTTAAGAAAAAAAAA AAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5042653 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5042653 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17165 same embryo
	EMAGE:17167 same embryo
	EMAGE:17168 same embryo
	EMAGE:17166 same embryo
	EurExpress:euxassay_001565 same experiment
	MGI:4828151 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source