EMAGE mouse gene expression spatial database

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EMAGE:17327

Pwp1 PWP1 homolog (S. cerevisiae) ( MGI:1914735)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003751. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003751_01	euxassay_003751_02	euxassay_003751_03	euxassay_003751_04

euxassay_003751_05	euxassay_003751_06	euxassay_003751_07	euxassay_003751_08	euxassay_003751_09

euxassay_003751_10	euxassay_003751_11	euxassay_003751_12	euxassay_003751_13	euxassay_003751_14

euxassay_003751_15	euxassay_003751_16	euxassay_003751_17	euxassay_003751_18	euxassay_003751_19

euxassay_003751_20	euxassay_003751_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17327_wholemount_strong.wlz

17327_wholemount_moderate.wlz

17327_wholemount_weak.wlz

17327_wholemount_possible.wlz

17327_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17327_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

trachea

strong

regional

strong expression: see section 12 13

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6962
Entity Detected:	Pwp1, PWP1 homolog (S. cerevisiae) ( MGI:1914735)
Sequence:	sense strand is shown >T6962 CTCAAGAGTAGTGTCTCCCAGCTCCCAGGGCTCCAACCTGCGCCGGGTTCAAACCGAAGATCATGAACCG CAGCCGCCAAGTGACCTGCGTGGCCTGGGTCCGCCGCGGCGTGGCCAAAGAGACTCCAGACAAGGTAGAG CTAAGTAAAGAGGAAGTGAACCGTCTCATCGCTGAAGCAAAAGGAAAGTTGCAAGAAGAAGGTGGGAGTG AAGAAGAGGAGGCAGGCAACCCTTCAGAAGATGGCATGCAGAGTGGCCCGACCCAGGCACCGCCTAGGGA GTCCCTGGAGGATGGGGATCCACAAGATGACCGCACACTGGACGATGACGAGCTGGCGGGGTACGACTTA GATAATTATGACGAAGAGGATAACCCAGATGCTGAGACCATTGGCGAGTCTCTGTTGGGTCTTACGGTCT ATGGCAGTAATGACCAGGATCCATATGTCACCTTGAAAGACACGGAACAATATGAGCATGAAGATTTCTT GATTAAGCCGACTGACAACCTCATCGTTTGTGGCCGTGCTGAACAAGAGCAGTGCAATCTAGAGGTGCAC GTTTACAATCAAGAAGAGGAGTCTTTCTACGTCCACCATGACATACTCCTGTCTGCATACCCTCTGAGTG TGGAATGGCTGAATTTTGATCCTAGCCCAGATGCATCTACTGGGAATTACATTGCTGTGGGAAACATGAC TCCTGTCATCGAGGTATGGGACCTGGATATAGTGGACTCTCTAGAACCAGTCTTCACACTGGGAAGCAAG CTTTCCAAAAAGAAGAAGAAGAAAGGGAAGAAGAGTTCCTCAGCAGAAGGACATACTGATGCTGTCCTTG ACCTATCCTGGAATAAGACAGTCAGAAATGTGCTTGCAAGTGCATCGGCAGACAGCACTGTAGTTCTCTG GGATCTGTCTGTGGGGAAGTCAGTAGCCAGGCTTACTGCACATACAGACAAGGTTCAGACTCTGCAGTTT CATCCATTTGAAGCACAGACTCTGATTTCTGGGTCCTATGACAAATCGGTGGCTCTGTATGACTGCAGAG ATCCCAGCCAGAACCACCGCCAGTGGCGGTTCAGTGGGCAGATCGAGAGAGTGACCTGGAACCACTTCTC ACCGTGTCATTTCTTGGCCAGTACAGATGATGGCTTTGTCTATAACCTGGATGCACGTTCAGACAAGCCA ATCTTTACACTTAATGCACACAACGATGAAATCTCTGGTCTTGATCTTAGCAGTCAGATCAAAGGCTGCC TTGTGACTGCTTCCGCTGACAAATTTGTGAAGATCTGGGACATCTTAGGAGATAGGCCAAGTCTCATTCA CTCTAGAGACATGAAAATGGGAGTTCTCTTTTGTTCCTCCTGCTGTCCTGACTTGCCATTTGTTTATGCC TTTGGAGGACAGAAAGAAGGGCTTCGGGTTTGGGACATAAGCACTGTGTCTTCAGTAAATGAAGCATTTG GAAGACGGGAGCGACTCGTTATAGGCGGCACGAAAGGTTTATCTGTTAGCGGCCCCTGTGGGAGCAGGAG TCCACAGCAGACACCAATGGAGTCCTGACAAAGATCAGTTTCCTGCTGTGCTTACCTTAACTCCGTTTCA AAAGACTGGCCTAAAAATGTCCACGTATATCTACAACCATAGATGGAAATTCAGGTCCTGTTGCCATCCT TTCTGCAGCCTTGGTGGCAGTCCACAGCCTTTGGGCTGCCTTCTACTGGGTACTGTACAGAGTTCTTGCC TTTCTTGAAAATATAAAGTTTTTTAAAAATGATTCCTTAACAGGCCATCTGACCTTTGGAACTCTAGGCA TAAAATACATGGTAACTGGAGAGTTTTTGATACGGGTCTTGCTGTGTTAGCAAAGCACTGGTGTGGTGGC TCGTGCTTGTCCCAGTCCTGGGGAGGAGAGAGAGACAGGCAGATTCCTAGGACCAGGCTGCCCAGCGAAT CTCACCTATCTCCATCTTCCCAGGCTAGGATTACCTATATGCATGGTAGGTTCTAGAATTGAATTCAGGC CCTCGTGTTGGCAAATCAAACATTTCACTAACTGAGCAGTGTCCCTAACCCATTGATAAATGTGATCCCT AAAGGTATTTTCACCTTTTGAATGTTAATCATATTTTGACAACTTTTGGTTTTAAACTTCCATGTTTTCA GGGATATTTTGTTTAAAATGTCAATTATTTTTTAAATGGGGATTTTGTTCATACAATTTTCTATGATCAA AATATTTTACTAAATAGACAACAAACTGTCAAAGTAATACATAACCTTGTAAGTTTTGGTCCCAGTACAT TCTCCCATCTATTTCCTTCAAATAAACTTGAACTTGTCAAGCCCAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3986908 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3986908 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17326 same embryo
	EMAGE:17325 same embryo
	EMAGE:17328 same embryo
	EurExpress:euxassay_003751 same experiment
	MGI:4827545 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source