EMAGE mouse gene expression spatial database

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EMAGE:17423

Erc2 ELKS/RAB6-interacting/CAST family member 2 ( MGI:1098749)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003848. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003848_01	euxassay_003848_02	euxassay_003848_03	euxassay_003848_04

euxassay_003848_05	euxassay_003848_06	euxassay_003848_07	euxassay_003848_08	euxassay_003848_09

euxassay_003848_10	euxassay_003848_11	euxassay_003848_12	euxassay_003848_13	euxassay_003848_14

euxassay_003848_15	euxassay_003848_16	euxassay_003848_17	euxassay_003848_18	euxassay_003848_19

euxassay_003848_20	euxassay_003848_21	euxassay_003848_22	euxassay_003848_23	euxassay_003848_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17423_wholemount_strong.wlz

17423_wholemount_moderate.wlz

17423_wholemount_weak.wlz

17423_wholemount_possible.wlz

17423_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17423_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

forelimb

moderate

regional

moderate expression: see section 01 02 03 04 05 06 22 23 24

hindlimb

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 21 22 23 24

adrenal gland

moderate

regional

moderate expression: see section 07 08 15

brain

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

adenohypophysis

strong

regional

strong expression: see section 09 11 12 13 14 15 not examined expression: see section 10

physiological umbilical hernia dermis

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

facial vii ganglion

strong

regional

strong expression: see section 06 07 18 19

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 05 06 17 18 19 20

trigeminal v ganglion

strong

regional

strong expression: see section 03 04 05 06 07 08 09 17 18 19 20 22

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 05 06 07 17 18

spinal cord

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13

cervico-thoracic ganglion

strong

regional

strong expression: see section 08 13 14

cervical ganglion

strong

regional

strong expression: see section 07 08 15 16

thoracic ganglion

strong

regional

strong expression: see section 09 12 13

dorsal root ganglion

strong

regional

strong expression: see section 05 06 07 08 09 10 14 15

retina

strong

regional

strong expression: see section 02 03 04 05 23 24 moderate expression: see section 01

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 06 10 11 12 13 14 15 16 17 18

esophagus

moderate

regional

moderate expression: see section 11 12

stomach

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09

midgut

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15 16 17 18

bladder

moderate

regional

moderate expression: see section 13

metanephros

moderate

regional

moderate expression: see section 06 07 08 09 15 16 17

lung

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 not examined expression: see section 02

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7809
Entity Detected:	Erc2, ELKS/RAB6-interacting/CAST family member 2 ( MGI:1098749)
Sequence:	sense strand is shown >T7809 CGGCGCTCGGCGCTGGCCGCGCTCCCCTCCACCTCCCCACCACCTCCCCGCGCTGCCCGCGCTCCACCGT CTCGCGCTCCTGATCGCTCCAGCTCCTCCCGGCCGCCCGACGCCGCAGCCACGCGAGGTTGTAAAAATCC ATGTGGGACCGCGCCGGGGCGAGCATCGCTAAGATCTCTGAGTGGAGTCATTTCCTGGTACAGACTTCAC AAAGAATGTGCTGAGAATTCTAGTCTGTGAAGGGAACATTGGCTTAAGCCCTCACGTGGCCTAGCATTTC TTCTCTTCAGTAGCACCACATCACAGAAGAAAAATGTACGGGAGCGCAAGAACAATCAGCAATCTGGAAG GCAGCCCTTCCAGGTCACCACGTCTGCCAAGGTCTCCTCGTTTGGGCCACCGAAGAACAAGCAGTGGGGG AGGGGGAGGGACAGGCAAGACTCTGTCTATGGAGAATATCCAGTCCCTTAATGCAGCCTATGCTACGTCT GGACCCATGTACTTGAGTGACCATGAAGGGGTGGCATCGACAACTTACCCAAAGGGAACCATGACTCTGG GCAGGGCGACAAATCGAGCGGTGTATGGAGGCCGGGTCACAGCCATGGGGAGTAGTCCCAACATTGCTTC CGCTGGACTTTCCCACACGGATGTCCTTTCCTATACGGATCAACATGGAGGGCTGAGCGGCTCATCCCAC CACCACCACCACCAGGTGCCCTCCATGTTGAGGCAGGTAAGAGACAGCACGATGTTGGATCTTCAAGCCC AGCTCAAAGAGCTGCAGAGAGAGAATGACCTCCTACGCAAGGAGCTTGACATTAAGGACAGCAAACTGGG GTCTTCCATGAACAGCATCAAGACTTTCTGGAGTCCTGAGCTGAAGAAGGAGAGAGTCTTGAGGAAAGAG GAGGCAGCTCGCATGTCTGTCCTCAAGGAGCAGATGAGGGTTTCTCACGAAGAAAACCAGCACCTGCAGT TGACCATCCAGGCCCTTCAGGATGAGCTGCGGACCCAGAGAGACCTCAACCACCTCCTGCAGCAAGAGAG CGGCAATAGAGGAGCCGAGCACTTCACCATTGAGCTGACAGAGGAGAACTTCCGCAGGCTCCAAGCTGAG CACGACAGGCAGGCTAAGGAGCTGTTCCTTCTGCGGAAGACATTGGAAGAAATGGAGTTGAGAATCGAAA CACAGAAACAGACTCTCAATGCCCGAGACGAGTCCATTAAAAAGCTCCTGGAGATGCTGCAGAGCAAGGG CTTGCCATCGAAAAGCCTTGAAGATGACAATGAGCGCACACGGCGAATGGCGGAGGCTGAGTCTCAGGTC AGCCACTTGGAAGTGATTTTAGACCAGAAAGAGAAGGAAAACATCCACCTGAGAGAGGAATTGCACCGAA GAAGCCAACTTCAGCCGGAGCCAGCCAAGACCAAGGCTCTCCAGACTGTCATCGAAATGAAGGACACAAA AATTGCTTCATTGGAGCGTAACATCCGGGACCTGGAGGATGAGGTCCAGATGTTAAAAGCCAATGGTGTG CTCAACACAGAGGACCGGGAGGAGGAGATCAAACAAATCGAGGTGTACAAAAGCCACTCCAAGTTTATGA AGACCAAGATTGATCAGCTGAAACAGGAACTTTCCAAGAAGGAGTCAGAACTTCTTGCCTTACAAACAAA GCTTGAAACCCTTAGCAATCAAAATTCAGATTGCAAGCAACACATTGAAGTGCTTAAAGAGTCGCTTACT GCCAAAGAACAGAGGGCTGCCATCCTTCAGACTGAGGTAGATGCACTGAGATTACGGCTGGAAGAGAAAG AATCTTTTCTCAATAAGAAAACAAAACAGCTTCAAGACCTCACCGAAGAGAAGGGGACCCTAGCTGGAGA GATCCGTGATATGAAAGATATGTTAGAAGTAAAGGAAAGGAAAATCAATGTTCTTCAGAAAAAAATTGAA AACTTGCAAGAACAACTTAGGGATAAGGACAAACAGCTGACCAACCTGAAAGACAGAGTGAAGTCCCTGC AGACAGACTCCAGCAACACGGACACAGCTCTGGCCACACTGGAGGAGGCCTTGTCGGAAAAGGAGAGAAT AATAGAGCGCTTGAAAGAGCAGAGGGAGAGAGATGATCGAGAAAGACTAGAAGAGATAGAATCCTTTCGA AAGGAGAACAAAGACCTCAAAGAGAAGGTCAACGCTTTACAGGCTGAGCTGACAGAGAAAGAGTCCAGTT TAATCGACCTCAAAGAACATGCATCCTCGTTAGCTTCTGCAGGACTGAAAAGGGACTCCAAACTGAAGTC TCTAGAAATAGCCATTGAACAAAAGAAGGAGGAATGTAACAAACTGGAGGCACAATTAAAAAAGGCACAT AATATTGAAGATGACTCCAGGATGAACCCCGAGTTTGCAGACCGACTAAAACAGCTTGACAAGGAAGCAT CTTACTACCGAGATGAGTGTGGCAAGGCTCAAGCAGAAGTGGACAGGTTGCTGGAGATCCTCAAGGAGGT GGAGAATGAGAAAAACGACAAAGACAAGAAGATCGCCGAACTGGAGAGGCACATGAAAGATCAGAATAAG AAGGTAGCCAACCTCAAGTACAACCAACAGCTGGAGAAGAAGAAGAATGCCCAGCTGTTAGAAGAAGTGC GCCGGCGAGAAGATAGCATGGTTGACAACTCGCAGCATTTGCAGATTGAGGAGCTGATGAATGCCTTGGA GAAAACCAGGCAGGAACTGGATGCCACCAAAGCACGCCTCGCCTCTACCCAGCAATCTCTGGCTGAAAAA GAAGCCCACCTGGCCAACCTCCGGATTGAGAGGAGGAAACAGCTGGAGGAGATCCTGGAGATGAAACAGG AAGCGCTACTTGCGGCCATCAGTGAAAAAGACGCAAACATTGCCTTGCTGGAGTTATCTGCCTCCAAAAA GAAAAAGACACAGGAGGAAGTCATGGCTCTGAAGCGGGAGAAAGACCGACTGGTGCATCAGTTAAAGCAG CAGACCCAGAACAGGATGAAGCTGATGGCAGACAATTACGATGAAGACCATCACCATTACCACCACCACC ACCATCACCACCACCACCGGTCTCCTGGGAGGTCACAGCATTCCAACCACAGGCCCTCTCCAGACCAGCT GTCAGAAGGGTTGGATAAAAGGATAGCTCAGCACTGCAGCAGTATCCTGATAATCTATTGCAGCCTGGCT CTCCTCACTATCCACCAGCGGCGGCCGGCCGTGGCAGCAGGTCTGAAAGGAAGGGGAGTTTTTGCCTTCA CTTTCCTGCTCAATTCTGTTCTCCTCGATTGATTTGAGTGTAAGGGAAAGAAAGAGTATGAAGAAGGAGA AAATGACAACCCCTTCGTTTTCTAACAAGGATTTGGACCTTTACGAAGTGAGCATCTCTGCGGAAAGCTG CGTAGCAAGCGTTTTGTTCCTGTTGCTTTGTCTCTTGGATACATGGGCACTACACGGGATTCAGAGTGCA GATTCAACTTCAGTTGGACAGCTAGCTCCCGTCTCCTGTTGTCCTGCTGGTCAGTCTAAGTGGTCCTAAC ACATTAGATTATCAGACAATCTAAGCTTGCTGTGAACAAGTGCTGCTGCTGGTGTTTGTATACCATGGTG CATGGAGTGTTTGACAAAGAAACCAAGAGACCAAAAATCGAGAGCCTTTACCCTCCTCCATCTCTTTAAT CTGTTGGTTAAGGAACCACATTGAACAACGGTATTATCAGTAGTTACCTCTGGGCCAGGTTGGAAATGAA ATCAATTTTTTTAAACCACTAATAACTTGCCAAGAAATCGCCTTATGAGAACATGGTGTGTGTCTCTGAC AGTGCATGGCACATCTAAATTGGTCATTCGTAATTTTATTGTCCTGCTCATGTACCAGAGAATGACAAAA GCCTGTGGCACCTGAATATCCACTCCACATGTTCCTTGAACCCCTCAGCTGGGGCATGACTTGGCCTCAG GCTCTTCTCTTTCTCATACATGCAGGTCTCCATGACAAAGCTGAGGTTCGTTCATGTTTCGAGATCCAAA CGCTGTGCAAGCAATGTGAGCCTGGATCTCCTCTAAATGAAAGGTCACTTCTCAGTCCTTTTTTGTTTTG TTTTGTTTTGTTTTAAGAAAATGTCTCCAACATGGATTCCAAAGCATTTTCAGCATCCAACATGAAAACA TTGATGAATGAAGAGCAATCACAAAAGCTGTATGTCTGTAAATAGTAATATACAACCTGTATGGAAACAG AGTCCTTATCTTAAAGCACATGCCAGGGAAAGAGACCGCACTCCGAGGTCTTTTGCATACAGTGACAAGG TACTAAGGAGAATTCAACTCCTGCTGCTTCCTCTGAAGATTAAAAAGTATTAGTCCTTACAACTCCGTAA ACACACACAACTGAGCCTGGTAAATGTGCAGCTCTTGTACTGACCCATTTTCCTGTCAATGGGAGAACCC CTCCCACCCACCTGCATTCCTAAAGGACAAACAATGAATTTAAGCATGAGTGGCCTTAATAAATACCCTT GTCTTTCTTTGCTGCTGTGGAAAGGGTTCTAAGCAGAAGTTGCTGAAATCTTGCATTGACTCTAATCATT ATTACCTTCCAAAATGTTGGTGCAATTAACTCTGGACCTCTCTCATGAGCCATAGAGATATTTAAAAGAC AAACCCCATATTTGCTGGGGAAAGGAAACTGTCCCTGGGCTGCACACTTTATTCCTGGTGCACCGGGGGT GATGTACTCAAGCCGGTTTCTTGCTTTGGGCTTCATTCTTGTCAGTAAATAGTGACCTGTGTAAAGCTTA TGTGTTACATAAATGATCCAAATACATTCATGTTTAAAAAAGTTTGTCCTTTAACTATCAAAAGAATAGG TCTTTGTTACAGAAGGGAGCAGGGTTCTTTGCAAGTTTATTTTGTACATTTTGGCAGAAGGGTGCAGTGA AAACCTAAGCCACAGAGGAGTCCCTAGGTATGGCTTGGATGTGGTCAGAGAAGCCCTCATGGCTTCCGGC CACCAGGTCCTGGCCTGAACTCTGCACCTAAATACTTGAAATTGGAAGTAGAAGAACCAGGATGGCAAGC AGGGTCCTTGAAGTAGCAACAAAGGGATCAACTCTGTGGTCAGCTGTCTGCATTCGTGCCAATTTTGACA CAGCTCCTGTTTGGGGAACATTTCTTTGCCCCACTTTTGCCGGTTGAAAGTATTATCGATCTTACAAGCA TATCATTCAGTCCCTGCAAAATCCAATTCTATCTAAGACAGCATCTTTTCCCATTTTGTGTACCATTCTC CAATATTTGGGGGTTTCCATCAAAAATATCCCTTTCCACACACTGAAAATTGTGTCTGGGTTTACAACAA ACACTGTGTTGTTCAGAGGATATTTCCTTTGTGAAAACTTTAGCAGGTTCCCTTCTAATCATAGTGTAGT TGAAATTAAACAAACAAACAAAAAGAAGTTTGGGCTAACATTTAGGAGAAGTTGGTCTAAGTCTTACCTT GTGTAAAAGCAGGCTTGTATTTATAACCTATTTGATTTCTATATACAGTCTCAATTTTGTATTTAATGAT TTTTGTGTATTAAGTATGCACGTTCACAGCGTGTCAACTTTCATTTGGAAGTGGGTTTTATTTTTTAAAC AGTGTTTATGATGAGCCCTCAAGCGTATTGATATAAGCTCTAGCTGCACCGTCTTTGTGTAGAGCGCCGT TTGAATGCTGCCAAGGGTCCCTGTATCTAATTCCCCGAGACCCTTGTTTGATAGTGCTTCTTGTAATATT TCTTCAAGTGAGTGGCATGTGGGTTGTGATATTGATCCATGTGATTATGGACATTGATATGAAAAAAAAT TTAAAAATAAATAAATAAAATTAAGGAACCCTCGAAACTATCACTGGGCATGTATCAGCCAGTCATTGTA ACCTCTTGTCTAGTAGAATACCCATGTGCAAGGTATGTACAGCTCATAACTTTGTACTGTGTGTATGAGG AGTGCTTTGTGCCGATTGTCTTCTTTATATTCATCAAATAAACCTTGTTTCTTTCTGAAGTGAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6331997 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6331997 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17424 same embryo
	EMAGE:17427 same embryo
	EMAGE:17422 same embryo
	EMAGE:17426 same embryo
	EMAGE:17425 same embryo
	EurExpress:euxassay_003848 same experiment
	MGI:4824610 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source