EMAGE mouse gene expression spatial database

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EMAGE:17493

Mrpl13 mitochondrial ribosomal protein L13 ( MGI:2137218)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003941. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003941_01	euxassay_003941_02	euxassay_003941_03	euxassay_003941_04

euxassay_003941_05	euxassay_003941_06	euxassay_003941_07	euxassay_003941_08	euxassay_003941_09

euxassay_003941_10	euxassay_003941_11	euxassay_003941_12	euxassay_003941_13	euxassay_003941_14

euxassay_003941_15	euxassay_003941_16	euxassay_003941_17	euxassay_003941_18	euxassay_003941_19

euxassay_003941_20	euxassay_003941_21	euxassay_003941_22	euxassay_003941_23	euxassay_003941_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17493_wholemount_strong.wlz

17493_wholemount_moderate.wlz

17493_wholemount_weak.wlz

17493_wholemount_possible.wlz

17493_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17493_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

weak

regional

weak expression: see section 07 08 09 10 17 18 19 20

olfactory cortex ventricular layer

weak

homogeneous

weak expression: see section 10 11 15 16 17

telencephalon ventricular layer

weak

homogeneous

weak expression: see section 02 03 04 05 06 07 08 09 10 11 12 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9742
Entity Detected:	Mrpl13, mitochondrial ribosomal protein L13 ( MGI:2137218)
Sequence:	sense strand is shown >T9742 GGTGCAAAGTAAAGGACGCCGCGGGTCGGAGAGCATGTCCAGCTTCTCTAGGGCGCCCCAGCAATGGGCC ACTTTTGCTCGAATGTGGTATCTCTTAGATGGCAAAATGCAGCCCCCTGGCAAACTTGCGGTTATAGCAT CCAACAAACTGCAAGGATTAAATAAACCAGTGTACCATCAGCTGAGTGACTGTGGAGACCACGTTGTCAT AATAAACACAAGACATATTGCATTTTCTGGAAACAAATGGGAACAAAAAGTGTACTCCTCACATACTGGC TACCCAGGCGGCTTTAGACAAGTAACAGCTGCGCAGCTTCACCGGAAAGACCCCGTGGCGATTGTGAAAT TGGCTATTTATGGCATGCTGCCCAAAAACCTGCACAGAAGGACAATGATGCAAAGGCTGCATCTTTTCCC AGACGAGGATATTCCGGAAGATATTCTTAAGAATTTAGTGGAAGAGCTTCCTCAGCCACGGAGAGTGCCT AAACGGCTGGACGAGTACACCCAAGAAGAAATAGAGGCTTTCCCCAGAGTGTGGACTCCACCTGATGATT TTCGGATGTAGGAGAATGGAAATGATAGAACATTACAGCAAGAGACTGAATGTTTCCTGAAGTATTACCA TGACCCAAGGCAGAGAGAAACCATGGGAACTCCCTGTGGGGAACACTCAAGAAGGCCAAGCCTCAGCAGG AAAGTGCAGTAGAAGATACTGTTTTACAGACAAGTCCTGTGTAACCTGCTTTAAATGCTACACATGTTAA TAAAATGCTTATTTGGTTAGCTAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6741102 using vector (pDNR-LIB) specific primers. Forward Primer - name:M13 forward (-21), sequence:TGTAAAACGACGGCCAGT; Reverse Primer - name:T3-pDNR-LIB rv, sequence:CAAATTAACCCTCACTAAAGGGTGTTCACTTACCTACTGGGC. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6741102 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17494 same embryo
	EMAGE:17491 same embryo
	EMAGE:17489 same embryo
	EMAGE:17492 same embryo
	EMAGE:17490 same embryo
	EurExpress:euxassay_003941 same experiment
	MGI:4826440 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source