EMAGE mouse gene expression spatial database

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EMAGE:17646

6330408A02Rik RIKEN cDNA 6330408A02 gene ( MGI:2447816)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005978. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005978_01	euxassay_005978_02	euxassay_005978_03	euxassay_005978_04

euxassay_005978_05	euxassay_005978_06	euxassay_005978_07	euxassay_005978_08	euxassay_005978_09

euxassay_005978_10	euxassay_005978_11	euxassay_005978_12	euxassay_005978_13	euxassay_005978_14

euxassay_005978_15	euxassay_005978_16	euxassay_005978_17	euxassay_005978_18	euxassay_005978_19

euxassay_005978_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17646_wholemount_strong.wlz

17646_wholemount_moderate.wlz

17646_wholemount_weak.wlz

17646_wholemount_possible.wlz

17646_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17646_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9558
Entity Detected:	6330408A02Rik, RIKEN cDNA 6330408A02 gene ( MGI:2447816)
Sequence:	sense strand is shown >T9558 CCCAGGTGGAGATGGGTCTGATGTCCAGCTCTTCTCCCCACTTCTACTCAAGGTCCCAGGATGGAGCTGA CAGAGCCCTTACCGAGCGCAGCCGTGCAGAAGGAGGAACAGGAATTGTTGGATCGAACTTTCTTCTCTTG GGCTGAGTTCAGCCGCTTCTTCGACAAGTGGTGCCAGCAGCGACTGGTAGTCTTCTCGGTGAAGAGCTCC ACACGTGTGGCACGAAGCCCCTGGGCCAACACGCCCCCACTCTACAGGCTTATCCACGTGCTCAAGTACA GCTACGTGCTGCTGGTGTGCAAGGATGTGCGCATGCCCAACAAGTCCACAGCGTGGCCTCCCCAGCCCAG CTGCCCTGCCTTCATCACTGTCAAGCTGAGCCCTTTGCGAGATCGACTCGTGGTGACTGAGTGCCAGCTG ACCCACTCACACCCAGCCTGCCCACGTGAGTTCGCCTACCACTTCCGCCCAGGCCACTTGCTGGCCAATT CCTGCCTGCCAGTCCGCATCACCAACCAGATCTCCAAACAGTTTGTGGCCCCTGCTGATGTGCGCCGCCT GCTGACCCATTGCAAGGGCCCAGACCATGGGGTCCTAGATGCCCTGCAAGTGCTTGAGGGGCTCTTCCGC ACAGATCCGGAGGCCAAGGTGAAGCTGGTGTTCGTGGAGGATCAAGCAATGGTAGAGACTGTGTTCCTTC TAACATCGCGCACCAGGGCGCTGCTGCGGCGCTTCCCACGCATCCTATTGGTGGACCGGCTACCTGGGTT GCAGGGCACCCTGGACCTGATGGCAGTACTGTGTGTGGACAGCGCTGGACGTGCGCGCCAGGCTGCCTGT TGCGTGGCGCGTCCCGGCACGCCTAGCCTACTGAGATTCATGCTGGTTTCCCTGCTGCAAAGCGCTCCTG ATGTCAAAGGCCGAGTGCGCTGCCTCACAGCTGGACCAGAGGTGGCCGGACAACTGCGTGCAGTTCGCCA GCTGTTGCCGTGTGCACGTGTGCAGATCTGTCGCGCTCAGGGTCTAGAGACGCTCTTCAGTAAGGCACAG GAGTTGGGTGGTGCCAGCCAGGAGGATCCGGACCTGTGGCCACTCCTCTGCCGCCTAGCAGATTCAAAAT CCTATACTGCTTACAGCGAGGCGCTGGCTGAGTTGCGTTCCCAGGCTCCAACTGCTTTCATCAGATATTT TGAGCACAACTGGGCGCCCCGCCACGACATGTGGGTACGTTTCCGTGCCTACGAAGCAACCCGTGACCTG GATGCCTGTGCTCTTGTGCGCAGCCATCGCAGGCGACTCTTAAGACGCCTCAGCCCCTCACATAGTGTAG CTCAGTGCCTTCGTGACCTAGTGGCCATGCAATGGGCAGATGCAACTGGAGAGGCAGCCTCAGAGGGTGT TAATGGCGACCGAGTGTTGTTGGAGAGTGAACCCAGGAATAGGGCAAGGATGGAGAAAGAGAAGGGGAGG GGCACAGAGACCAGCCACTGTGGAAGGCCTCATCGAGAGAGTGAGAAAGGGAGGCGACTGCACTTGAGAG ATGGTCAAGGAGTCTGGTTGGAGAATCAGAAGGTGAGGGGTCGTGAAGGGACAGGAGCCCAGCTGGAAAA AGAGCTTTTGAGAGAGCCAGAGGTCAGAGACTGGAGGGGAACCCAGATAGAGAATAGGGTAAGGGTGCTG GGGCCCATAGATCGGAGAGGCAACCTGGTTGAGTCTGAGAATGCCAAGAGTCTTGAAGGAAGGCCCTGGA GAGACGTACAGTTGCAGGAGGAGAGGGCACGTGCACCAAAACCTCAAGACTGGAAAGAGCCTCCGTTTGA AATAGAGAAGGGGATCGACGTTAGAAAGTGGAGGGAGGTCCATGTGGAGAAGCCCTTAGAGTTAGTCCCC AAGAACGGAGATCAGAGGGGAGCTGAGGGGCAGAAAGGGCCGGAGACTGCAGAGGAGAGAAGTGGGAGGT TTCTAGTCAAAAGGAGGCGGGGCCTGGAGGACATTGTTGTTGTTCATTTAGAGGACGCAAGAGCAACAGG CATAGTAAATGGAGATGGCAGGGGACCCCAACCTGTGAGTTTCAAGAATGGAGGACAGCAGGTGGAGTTT GGGGACCCTGGGGGGAGGCTTCTAGGGATGGGGAATGGTGTACCATGCACCACCCCTGTGCAAACTGTGT TGAAAGGCAATCCAGAATGGGCAGTGAACAGGGTTGAGTCCCTGGCTGCAGGGGAGGCAGTACAGGGAGG AAGTGAAGAAGGCCCAGGGGAACCAAAGAGACTTTGCCTCCCCTGTAGAGATGAGGGAGTGGACTGTGAG CTACTAGCCAAGTTCCGAGCAGCCTGTGGGCCAGAGTTGGCAGATCTAGTGGCAGAGGAGCTAGCCTTCG CTAGGCAACATGGGACCCGAGGTTTCCACTGGACCGGAGCTGGCTTTGCCCTTAAGGATGGCACCTCAGA CTTCTTCCTGGATGGGGCCCTGACACACTGCAGCTGCTCAATCTATGCTGCCAGATGCCTGCCCTGCAGA CACCTCTTTGCTGCACGTCTCCTCACAGGGGCAGCCTTGTTCCACATGGACCTGCTCAGAGATTGCTGGG GAAGATCTCAGGAGCCCTGACCCTGTGTACCCCTGCTTGCTGCCCTCCACATGGAGGGCAAGAACATAGT CTTTGATCCCAAAGATAATAGGGTTTATGCCCTGCCCGGTGGGTCACCCGAGCACTTCTAATCACCTCCT GAGTCATCTAAAGACCTCCTCTGGCCTTCTTTCTCTACATCCTAGGGAATGCTGGCTATTATCTCTGAGA TCCTGGAGCCACACCTGGGGAAGTGCTAATGGCCAGGACATATCGAAAGGACATTGCCCTGGAAAATAAA TGAGGAAACGAAGAGGTTATGAGAGACAGGGTCAGCCAAGAAAGGGATAGAGCTGGAGCAGGGGGATGCA CAGAGGAGGTCATGGAAAGAAGCTAAGCTATGGGGACTGACAAGAGTAAGGATGGAGAGCCCTAGAGACT ACAAGGTTGAAATGGGAAAAGCCAGGAAGAATGAGCAAAACTGGAGAAAGGATAGGAGGGAGACAAGAGA TAGGGCTGGGGAAGTGAAGCATGGAAGGAGAGAGGGTGAGAAGGGGTGAAGACAGTTTTTAAAGGGAGAT GACAAAGCAGAGGAAGACAGAAGATGATAGGATGACCTGGGGAGTTGGCCATTTCTCAGGGTGGTGCGGG GGAGATGGCTCTCTGGTGGGACAACCTGCATACTGCCTCAGAGGGAAGGGAACTAAGTGAACAGCTGTAC TTTTAGATTGAAATAAACAGCATTATTTTGAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30608389 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30608389 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17648 same embryo
	EMAGE:17647 same embryo
	EurExpress:euxassay_005978 same experiment
	MGI:4822776 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source