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EMAGE:17834

Rap1gap2 RAP1 GTPase activating protein 2 ( MGI:3028623)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:17834 EMAGE:17834 EMAGE:17834 EMAGE:17834 EMAGE:17834
"Pseudo-wholemount" of euxassay_009038. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_009038_01 euxassay_009038_02 euxassay_009038_03 euxassay_009038_04
EMAGE:17834 EMAGE:17834 EMAGE:17834 EMAGE:17834 EMAGE:17834
euxassay_009038_05 euxassay_009038_06 euxassay_009038_07 euxassay_009038_08 euxassay_009038_09
EMAGE:17834 EMAGE:17834 EMAGE:17834 EMAGE:17834 EMAGE:17834
euxassay_009038_10 euxassay_009038_11 euxassay_009038_12 euxassay_009038_13 euxassay_009038_14
EMAGE:17834 EMAGE:17834 EMAGE:17834 EMAGE:17834 EMAGE:17834
euxassay_009038_15 euxassay_009038_16 euxassay_009038_17 euxassay_009038_18 euxassay_009038_19
EMAGE:17834 EMAGE:17834 EMAGE:17834 EMAGE:17834
euxassay_009038_20 euxassay_009038_21 euxassay_009038_22 euxassay_009038_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:17834Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
17834_wholemount_strong.wlz
17834_wholemount_moderate.wlz
17834_wholemount_weak.wlz
17834_wholemount_possible.wlz
17834_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:17834_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
pancreas
moderate moderate
regionalmoderate expression: see section 06 07 08 11 12
nasal cavity olfactory epithelium
moderate moderate
regionalmoderate expression: see section 09 10 11 12 13 14 15 16 17 18
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T36419
Entity Detected:Rap1gap2, RAP1 GTPase activating protein 2 ( MGI:3028623)
Sequence:sense strand is shown

>T36419
CAGATAGAAGGCCTTAGCCAGAGTTTCTCTCTCTGTGATAATGAATGTCAAATGGTGTTTCTTGGAAAAC
CATCTGGACTTCAGTTTTGTGGGCCCTTTTTGATACTCAAGCCTCCTAGGCCCTGTTTGCATCTCAACAG
ACCATGTCATCCTCCAGATGAACAGAAAGAACTTGCCTGAGGCCACACAGCCGCCTTGAGCCCGTTCCTG
CATACCAACTGTACTTACTGTTGGCACAGCAAGCAGTTGCATTTCCTGGAGCCTGGGTTTTGAGGAAGCC
TGGTAGCTGGGGTCTCTGCACTGGCGGGCAGGTGCTGAGGTCCGAGGTATTGATAGGGCCTCTGCCTCTG
CTCTTTTCCAGCATGACAAGCTTCGATCAGTGAGGACCCGGGGGCTTGGGAAATGGAGGAGGAGGGATTG
CAGTCAAGTGCTTCTTTGGGTTTTCAGTGACTGGGACAAAGTCATCTGGACAAAATGCATTAGCTACCCT
TGACTTCTTTGTTCGAAATCAGGACAGAAGATGATAAAATGAACTAATTCTCTGATATATCTTTTCTTCC
TCCCTCTCTCCATCCCTCCCTTCCTCATTTTTTGGTGGTGCTGGGAACGTCCCCAAGGCCTTGCTCATGC
TGAGTGAGGGCTCTGCCGCTGAGTCTAGGCCCAGGCCTCTCCAGGTTTTAAAATGGAGATGGGTAATAAA
AGCCCAGCTCAGGTGACCAGGCAGGGCACAGCCAGTGTCATTTTCTTTTCTTTGACCCTTTATGGTGCCA
ACTCAAACATCACCTTTTGTCCTTTGTTTCTTGGTCTGCAAAGACAGCTGTGTTGTGTGTACGTGTGTTG
GGTCCTGGGGTTCCGATAGAACATATGGCTTCTGGGC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 87541. Forward Primer - name:087541_F_cDNA_Garnl4, sequence:CAGATAGAAGGCCTTAGCCAGA; Reverse Primer - name:087541_N_SP6_cDNA_Garnl4, sequence:GCCCAGAAGCCATATGTTCTAT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:17835 same embryo
 EMAGE:17838 same embryo
 EMAGE:17839 same embryo
 EMAGE:17837 same embryo
 EMAGE:17836 same embryo
 EurExpress:euxassay_009038 same experiment
 MGI:4827608 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS