EMAGE mouse gene expression spatial database

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EMAGE:17908

Srl sarcalumenin ( MGI:2146620)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_009606. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_009606_01	euxassay_009606_02	euxassay_009606_03	euxassay_009606_04

euxassay_009606_05	euxassay_009606_06	euxassay_009606_07	euxassay_009606_08	euxassay_009606_09

euxassay_009606_10	euxassay_009606_11	euxassay_009606_12	euxassay_009606_13	euxassay_009606_14

euxassay_009606_15	euxassay_009606_16	euxassay_009606_17	euxassay_009606_18	euxassay_009606_19

euxassay_009606_20	euxassay_009606_21	euxassay_009606_22	euxassay_009606_23	euxassay_009606_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17908_wholemount_strong.wlz

17908_wholemount_moderate.wlz

17908_wholemount_weak.wlz

17908_wholemount_possible.wlz

17908_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17908_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diaphragm

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 15 16 17 18 19 20 21 22 23 24

forelimb

strong

regional

strong expression: see section 01 02 03 04 05 06 not examined expression: see section 07 08 09 10 11

hindlimb

strong

regional

strong expression: see section 01 02 03 04 05 06 07 10 11 12 not examined expression: see section 08 09

vertebral axis musculature

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

pons ventricular layer

strong

regional

strong expression: see section 11

midbrain ventricular layer

strong

regional

strong expression: see section 12

tongue muscle

strong

regional

strong expression: see section 11 12 13 14 15 16 17

tail mesenchyme

strong

regional

strong expression: see section 17 18 19 20 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T37079
Entity Detected:	Srl, sarcalumenin ( MGI:2146620)
Sequence:	sense strand is shown >T37079 AGATCACAGAGGTGTTGGGTTTCAGAGAGGACAATGGAGACCTTGCTCCTAGTCCTTGGAGGCTTCTCTG GACAGACATTCTCCCAAGTTTCAGGGGAAGCTGTGTCCACAGCTTCTTTGTGGGAGCACTTGATAGAGGA CATGTCTCTTCTGGTTCCCAGAACCATGGTTTCTCTTTTCCTTTCTTCTCCATTCCCTGATGCATTAATA CTTCCAACTGAGAAAAAGTCCCTGAATCAGAACCAGTACCAGCCTTGGCATTGGCACTGGTGCCAGATAA GACCTGAGGTTCAGGTTCAACCTGAACTTCTGCAGATGCCCACTGAATGCAGTCAGGACAGCCAGGACCT TCAAACACCAGTGAATGTGCACTTGAGGGCGTCAGACTTGAGGGCACAGAGAGAGGACTCCAGAGAGTCT TGCATGGCACACATACATTGCCACCTTGAAGTGCAGGGGAGTGAGAGGCTCTGGGAGGATGAAGAGATTT TTGATGGAGCGGGCAAGGTGAAGTTCAGCCTGCCTTGAGGGCTTTCTCTGAGCACAGCCAGCTGCTTTTT TGGGAGCAAGCTCTCCCGGGGGCCTTAGTTGTGAGGCATGGGTTGGGATCCGCCAGCACCAGGGTGTCAT CTAGAACAGA
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 72412. Forward Primer - name:072412_F_cDNA_Srl, sequence:AGATCACAGAGGTGTTGGGTTT; Reverse Primer - name:072412_N_SP6_cDNA_Srl, sequence:TCTGTTCTAGATGACACCCTGG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17907 same embryo
	EMAGE:17909 same embryo
	EMAGE:17906 same embryo
	EMAGE:17905 same embryo
	EurExpress:euxassay_009606 same experiment
	MGI:4828443 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source