EMAGE mouse gene expression spatial database

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EMAGE:17917

Svil supervillin ( MGI:2147319)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011675. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011675_01	euxassay_011675_02	euxassay_011675_03	euxassay_011675_04

euxassay_011675_05	euxassay_011675_06	euxassay_011675_07	euxassay_011675_08	euxassay_011675_09

euxassay_011675_10	euxassay_011675_11	euxassay_011675_12	euxassay_011675_13	euxassay_011675_14

euxassay_011675_15	euxassay_011675_16	euxassay_011675_17	euxassay_011675_18	euxassay_011675_19

euxassay_011675_20	euxassay_011675_21	euxassay_011675_22	euxassay_011675_23	euxassay_011675_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17917_wholemount_strong.wlz

17917_wholemount_moderate.wlz

17917_wholemount_weak.wlz

17917_wholemount_possible.wlz

17917_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17917_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

epidermis

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

vibrissa

weak

regional

weak expression: see section 03 04 05 06 20 21 22 23

diencephalon meninges

moderate

regional

moderate expression: see section 15 16 17 18 weak expression: see section 07 08 09 10 11 12 13 14

telencephalon meninges

moderate

regional

moderate expression: see section 15 16 17 18 20 weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 19 21 22 23 24

hindbrain meninges

moderate

regional

moderate expression: see section 15 16 17 18 weak expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 19 20

midbrain meninges

weak

regional

weak expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18

spinal cord meninges

weak

regional

weak expression: see section 06 07 08 09 10 11 12 13

pharyngo-tympanic tube

moderate

regional

moderate expression: see section 01 02 21 22 23 24 weak expression: see section 20

tongue muscle

weak

regional

weak expression: see section 09 10 11 12 13 14 15

stomach

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 14 15 16 17

midgut

weak

regional

weak expression: see section 09 10 11 12 13

exoccipital bone

moderate

regional

moderate expression: see section 01 02 03 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T37101
Entity Detected:	Svil, supervillin ( MGI:2147319)
Sequence:	sense strand is shown >T37101 GTCATACGAAGGAGGTTGGAAGGACTGCAGCAAATAAGATCAAGGAAGAATGTCCCCTGGAAGCAGGTTT GCACAGCAGCAGCAACGTGACAATACATGAGTGTGACGAAGGATCCGAGCCCCTGGGATTCTGGGACGCT CTGGGGAGGAGGGACAGAAAGGCCTATGACTGCATGCTTCAAGATCCTGGAAGTTTTAACTTCGCGCCCC GCCTGTTCATCCTCAGCAGCTCCTCCGGAGACTTCTCTGCGACAGAGTTTGTGTACCCCGCACAAGCGCC CTCTGCCGTCAGCTCCATGCCTTTCCTGCAGGAGGACCTGTACAGCGCGCCGCAGCCAGCTCTCTTCCTT GTTGACAACCATCACGAGGTGTACCTCTGGCAAGGCTGGTGGCCCACTGAAAACAAGATAACCGGCTCTG CCCGCATTCGCTGGGCCTCAGACCGGAAGAGTGCCATGGAGACGGTCCTGCAGTACTGCCGAGGAAAGAA TCTCAAAAGGCCACCCCCCAAGTCTTACCTTATTCATGCTGGGCTAGAGCCCCTGACATTCACCAACATG TTTCCCAGCTGGGAACACAGAGAAGACATTGCTGA
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 92959. Forward Primer - name:092959_F_cDNA_Svil, sequence:GTCATACGAAGGAGGTTGGAAG; Reverse Primer - name:092959_N_SP6_cDNA_Svil, sequence:TCAGCAATGTCTTCTCTGTGTTC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17916 same embryo
	EMAGE:17914 same embryo
	EMAGE:17915 same embryo
	EMAGE:17913 same embryo
	EurExpress:euxassay_011675 same experiment
	MGI:4828548 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source