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EMAGE:17928

Stab2 stabilin 2 ( MGI:2178743)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:17928 EMAGE:17928 EMAGE:17928 EMAGE:17928 EMAGE:17928
"Pseudo-wholemount" of euxassay_011691. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_011691_01 euxassay_011691_02 euxassay_011691_03 euxassay_011691_04
EMAGE:17928 EMAGE:17928 EMAGE:17928 EMAGE:17928 EMAGE:17928
euxassay_011691_05 euxassay_011691_06 euxassay_011691_07 euxassay_011691_08 euxassay_011691_09
EMAGE:17928 EMAGE:17928 EMAGE:17928 EMAGE:17928 EMAGE:17928
euxassay_011691_10 euxassay_011691_11 euxassay_011691_12 euxassay_011691_13 euxassay_011691_14
EMAGE:17928 EMAGE:17928 EMAGE:17928 EMAGE:17928 EMAGE:17928
euxassay_011691_15 euxassay_011691_16 euxassay_011691_17 euxassay_011691_18 euxassay_011691_19
EMAGE:17928 EMAGE:17928 EMAGE:17928 EMAGE:17928 EMAGE:17928
euxassay_011691_20 euxassay_011691_21 euxassay_011691_22 euxassay_011691_23 euxassay_011691_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:17928Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
17928_wholemount_strong.wlz
17928_wholemount_moderate.wlz
17928_wholemount_weak.wlz
17928_wholemount_possible.wlz
17928_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:17928_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
liver lobe
strong strong
single cellstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T37082
Entity Detected:Stab2, stabilin 2 ( MGI:2178743)
Sequence:sense strand is shown

>T37082
GCAACCTGTATGGAGAACAACACATGTGTGTGTAACTTGAATTACGAAGGTGACGGAATCACATGCACAG
TCGTGGATTTCTGCAAACAGAACAATGGGGGCTGTGCGAAGGTCGCCAAGTGCTCCCAGAAGGGCACACA
AGTCTCCTGCAGCTGCCAGAAGGGCTACAAGGGGGATGGCCACAGCTGCACAGAGATAGACCCCTGTGCA
AACGGTGTCAATGGTGGATGTCATGAGCATGCTACCTGCAGGATGACGGGCCCAGGCAAGCAGAAGTGTG
AATGTAAAAGTCACTATGTCGGGGATGGACGGGACTGTGAGCCCGAACAGCTGCCCCTTGACCGCTGCTT
ACAGGACAACGGACAATGCCACCCAGATGCCAACTGTGTGGACCTTCATTTCCAGGACACTACTGTTGGC
GTATTCCACCTACGCTCCCCACTGGGCCAGTACAAACTGACATTTGACAAAGCCAAAGAGGCCTGTGCTA
AGGAAGCTGCATCCATAGCCACCTACAACCAGCTCTCCTATGCCCAGAAGGCCAAGTATCACCTCTGCTC
AGCTGGCTGGCTGGAGAGTGGGCGGGTTGCCTACCCCACGATCTACGCCTCTAAGAAATGTGCAAACATT
GTGGGGATTGTAGACTACGGAACCAGGACCAACAAGAGTGAAATGTGGGACGTCTTCTGTTACAGAATGA
AAGATGTTAACTGCACCTGCAAGGCAGGCTACGTGGGCGATGGCTTCTCCTGCAATGGGAACCTGCTGCA
AGTCCTCATGTCCTTCCCCTCACTCACGAACTTCCTGACAGAGGTGCTGGTTTTTTCCAGGAGCTCAGCC
CAAGGCCGGGCGTTTTTGAAACACCTGACTGACCTGTCCATCAGTGGC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 66805. Forward Primer - name:066805_F_cDNA_Stab2, sequence:GCAACCTGTATGGAGAACAACA; Reverse Primer - name:066805_N_SP6_cDNA_Stab2, sequence:GCCACTGATGGACAGGTCAGT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:17926 same embryo
 EMAGE:17924 same embryo
 EMAGE:17925 same embryo
 EMAGE:17927 same embryo
 EurExpress:euxassay_011691 same experiment
 MGI:4828473 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS