EMAGE mouse gene expression spatial database

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EMAGE:17945

Tex15 testis expressed gene 15 ( MGI:1934816)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011704. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011704_01	euxassay_011704_02	euxassay_011704_03	euxassay_011704_04

euxassay_011704_05	euxassay_011704_06	euxassay_011704_07	euxassay_011704_08	euxassay_011704_09

euxassay_011704_10	euxassay_011704_11	euxassay_011704_12	euxassay_011704_13	euxassay_011704_14

euxassay_011704_15	euxassay_011704_16	euxassay_011704_17	euxassay_011704_18	euxassay_011704_19

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17945_wholemount_strong.wlz

17945_wholemount_moderate.wlz

17945_wholemount_weak.wlz

17945_wholemount_possible.wlz

17945_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17945_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon meninges

weak

regional

weak expression: see section 08 09 10 11 12 13 14 15 16

telencephalon meninges

weak

regional

weak expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

hindbrain meninges

weak

regional

weak expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17

midbrain meninges

weak

regional

weak expression: see section 05 06 07 08 09 10 11 12 13 14 15 16

spinal cord mantle layer

weak

regional

weak expression: see section 09 13

spinal cord meninges

weak

regional

weak expression: see section 08 09 10 11 12 13 14 15 16

saccule mesenchyme

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 16 17

utricle mesenchyme

moderate

regional

moderate expression: see section 01 02 03 04 05 17 18 19

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 09 10 11 12 13 15 16

vomeronasal organ

strong

regional

strong expression: see section 13 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T37128
Entity Detected:	Tex15, testis expressed gene 15 ( MGI:1934816)
Sequence:	sense strand is shown >T37128 ATTGACTTGGTGCCACATACTGCATCTGTAAATTTTGGAAACACTGTGGCAGAATTAGAACATAACTACA AGCAGTTTTTTCTATTACTCAAAAATGTAATGTCTGTCCCTCAGAAAGATTTTGGAAAAATGGTTCATAT TATAAAAGTTATGAAGACAATTGAACATATGAAGCTGCTAAGTGCTAAAGATACTAAATTGTCCACTCAT CTTCTCTTTCTCCAAATGCTGCGCAACAAAAGGAATGCTTTGCAACAAAACAGACAAGAAAAGATGGAGA CACCCGTTACAGAACCTGGGGAGGACAGCAGTCAACCTGGGGTTTCTGAGCAGACACCTCCAGGTACAGA GTGCACAGTAAAAAACATTTCAGACTCCTCTAAAAAGCGACCTGTGACTGCAGACACATGTGAAGTCTCT CAGGGAAAAGGAAATACAGACACTGTTCCCAGTTGGAAAAAACAAAAGGTTACCATGAAAGATGTTGGAA ACATACAGACAGTATCCAAACATCCAAGCACTACAGGATCTCCTCCCAATGATGAAAACAAAATAGGATC AAATTCCTCTGACAGTCTGAAAAGCATCTCTGCATCTCCAGAAGTGGTCAAAAGACAGAGCTCAGTACTT GGTTCAGTGTCACCTGCTGAAAGTGTACAAGACACTTGCACACCAAAGTCAGAAAGCAAAGTAGAGCCAA CAGACAGCTTACCTGATTCTTTAGCATCTCTCACTGAACAGCAGGAAAACTCAAATGTCATAGAGAAAAG AAATGGGAATTCTAGTGTGGCTGAAACAAATGATAAGAAAGACTGTCCTTTAGTAACTTGTGACCAAAAG GATATAGATGCCTCTTACTCACCTGACCACACACCTG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 66325. Forward Primer - name:066325_F_cDNA_Tex15, sequence:ATTGACTTGGTGCCACATACTG; Reverse Primer - name:066325_N_SP6_cDNA_Tex15, sequence:CAGGTGTGTGGTCAGGTGAGT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17944 same embryo
	EMAGE:17943 same embryo
	EMAGE:17942 same embryo
	EMAGE:17947 same embryo
	EMAGE:17946 same embryo
	EurExpress:euxassay_011704 same experiment
	MGI:4828666 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source