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EMAGE:18006

E130119H09Rik RIKEN cDNA E130119H09 gene ( MGI:1925800)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:18006 EMAGE:18006 EMAGE:18006 EMAGE:18006 EMAGE:18006
"Pseudo-wholemount" of euxassay_011759. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_011759_01 euxassay_011759_02 euxassay_011759_03 euxassay_011759_04
EMAGE:18006 EMAGE:18006 EMAGE:18006 EMAGE:18006 EMAGE:18006
euxassay_011759_05 euxassay_011759_06 euxassay_011759_07 euxassay_011759_08 euxassay_011759_09
EMAGE:18006 EMAGE:18006 EMAGE:18006 EMAGE:18006 EMAGE:18006
euxassay_011759_10 euxassay_011759_11 euxassay_011759_12 euxassay_011759_13 euxassay_011759_14
EMAGE:18006 EMAGE:18006 EMAGE:18006 EMAGE:18006 EMAGE:18006
euxassay_011759_15 euxassay_011759_16 euxassay_011759_17 euxassay_011759_18 euxassay_011759_19
EMAGE:18006 EMAGE:18006 EMAGE:18006
euxassay_011759_20 euxassay_011759_21 euxassay_011759_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:18006Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
18006_wholemount_strong.wlz
18006_wholemount_moderate.wlz
18006_wholemount_weak.wlz
18006_wholemount_possible.wlz
18006_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:18006_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
lens
strong strong
regionalstrong expression: see section 01 02
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T37688
Entity Detected:E130119H09Rik, RIKEN cDNA E130119H09 gene ( MGI:1925800)
Sequence:sense strand is shown

>T37688
CTTACCCGTCACATCTTCAGTGGCAGCGTGTCTGCCAAATTGCTCTGTTTTGTTTCATCACAGCATTCAC
CTCTACCTGACATTTCCTCGGCTCCTTTGTCATTGGCTGTCTCCCTCTGCTTACACGCCCACTGTGGAAA
GCAGTGACTTGCTTGCCTTGGCTACTACCACAGCCCACAGAGTCGGACTCCTGAAAATCAGAAAACAATC
GATTCGGAAGCACTCACTTCCTAGACTGCCCGTCACTCAGGCTGCCCAGACCCCGACTCCAGACCTGATG
AGAGTTTCAAGGGAAAGAGAAGATCCAACTGCCTCGTGCTTCTCTCCTTCTTAGCACATTGAGAAACTGT
AGCCTCAAGCCTGAAATCCTTTTATAAGGAGAACGATAGACCAGTTCTGT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 84201. Forward Primer - name:084201_F_cDNA_E130119H09Rik, sequence:CTTACCCGTCACATCTTCAGTG; Reverse Primer - name:084201_N_SP6_cDNA_E130119H09Rik, sequence:ACAGAACTGGTCTATCGTTCTCC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:18000 same embryo
 EMAGE:18001 same embryo
 EMAGE:17998 same embryo
 EMAGE:18002 same embryo
 EMAGE:18003 same embryo
 EMAGE:18004 same embryo
 EMAGE:18005 same embryo
 EMAGE:17999 same embryo
 EurExpress:euxassay_011759 same experiment
 MGI:4824446 same experiment
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS