EMAGE mouse gene expression spatial database

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EMAGE:18138

Mdfi MyoD family inhibitor ( MGI:107687)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011949. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011949_01	euxassay_011949_02	euxassay_011949_03	euxassay_011949_04

euxassay_011949_05	euxassay_011949_06	euxassay_011949_07	euxassay_011949_08	euxassay_011949_09

euxassay_011949_10	euxassay_011949_11	euxassay_011949_12	euxassay_011949_13	euxassay_011949_14

euxassay_011949_15	euxassay_011949_16	euxassay_011949_17	euxassay_011949_18	euxassay_011949_19

euxassay_011949_20	euxassay_011949_21	euxassay_011949_22	euxassay_011949_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18138_wholemount_strong.wlz

18138_wholemount_moderate.wlz

18138_wholemount_weak.wlz

18138_wholemount_possible.wlz

18138_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18138_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 16 17 18 19 20 21 22

radius

moderate

homogeneous

moderate expression: see section 23

humerus

moderate

homogeneous

moderate expression: see section 01 02 03 18 19 20 21 22 23

fibula

moderate

homogeneous

moderate expression: see section 01 02 23

tibia

moderate

homogeneous

moderate expression: see section 01 02 23

femur

moderate

homogeneous

moderate expression: see section 02 03 04 05 06 17 18 19 20 21 23

naris

moderate

homogeneous

moderate expression: see section 08 09 12 13

nasal septum

moderate

homogeneous

moderate expression: see section 10 11

viscerocranium

moderate

homogeneous

Expression in the turbinate bone.

meckel's cartilage

moderate

homogeneous

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 15 16 17 19 20 21

axial skeleton

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14

basioccipital bone

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 07 08 09 10 11 12 13 14 15 16 17 18 19 20

basisphenoid bone

moderate

homogeneous

moderate expression: see section 10 11 12 13 14

temporal bone petrous part

moderate

homogeneous

moderate expression: see section 01 02 18 19 20 21 22

vault of skull

moderate

homogeneous

moderate expression: see section 01 02

orbito-sphenoid

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 16 17 18 19 20 21 22

scapula

moderate

homogeneous

moderate expression: see section 02 03 19 20 21

pelvic girdle skeleton

moderate

homogeneous

moderate expression: see section 07 08 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30012
Entity Detected:	Mdfi, MyoD family inhibitor ( MGI:107687)
Sequence:	sense strand is shown >T30012 TGGGCTGGAGGTAGCAAGATCCACTCACCCTGTAGAGGCATCTTCTGAAGAGGGCTTCCCGGAGGAGGCG GCACCCTCCATGCCCCATGACAGTGGTCTCCGGGCTCAGCAGGCTCTGAACAGCATTGACCTCGATGTCC CCACAGAAGCTGTGACGTGCCAGCCTCAAGGGAACCCCCAAGGCTGCACCCCACTACTGCCAAATGGCTC CAGCCACGACCACCTCTCAGAACCGGGCAGTGCAGGGCATGCGGGGAACGGTGCTCTGGGCGGGTCCAAG GCCCACCGGAAGTTGCAGACGCATCCATCTCTGGGCAGCCAGGCTGGAAGGAAAAGCAGAGGCAGCGCCC GGTCAGCCTCACAGGTCCCTCTCCAGGCACAGGAAGATTGCTGCGTCCACTGCATACTGTCCTGTCTATT CTGTGAGTTCCTGACGCTCTGTAACATCCTCCTGGACTGCGCCACCTGTGGCTCCTGCAGCTCTGAGGAC TCCTGCCTCTGCTGCTGCTGCTGTGGGTCCGGCGAGTGCGCGGACTGTGACCTGCCCTGCGACCTGGACT GCGGCATCGTGGATGCCTGCTGCGAGTCCGCAGACTGCTTGGAGATATGCATGGAGTGCTGTGGACTCTG TTTCTCCTCCTGATGCCATGGGGTGGCCCCAGAGCTACCGCACAAAGCTTGACGCCTCCCCTGACCCCGG GCCGCCCCCTCAGAATCCCAACCCAGATGTGAGAAGGTGGGACGCTCAGAGGGGCCACCTCAGCCACCGA ACAGGTCTGCTTTCAGACGCGTAGCCTGGTCCCCTCCACGGGTGACCAGGAACACGGCAT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3488088), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 10791. Forward Primer - name:010791_F_IRAV12-15_E23_Mdfi, sequence:TGGGCTGGAGGTAGCAAG; Reverse Primer - name:010791_R_SP6_IRAV12-15_E23_Mdfi, sequence:GATGCCGTGTTCCTGGTC. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18140 same embryo
	EMAGE:18135 same embryo
	EMAGE:18139 same embryo
	EMAGE:18137 same embryo
	EMAGE:18136 same embryo
	EurExpress:euxassay_011949 same experiment
	MGI:4826139 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source