EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:18144

Adrm1 adhesion regulating molecule 1 ( MGI:1929289)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011943. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011943_01	euxassay_011943_02	euxassay_011943_03	euxassay_011943_04

euxassay_011943_05	euxassay_011943_06	euxassay_011943_07	euxassay_011943_08	euxassay_011943_09

euxassay_011943_10	euxassay_011943_11	euxassay_011943_12	euxassay_011943_13	euxassay_011943_14

euxassay_011943_15	euxassay_011943_16	euxassay_011943_17	euxassay_011943_18	euxassay_011943_19

euxassay_011943_20	euxassay_011943_21	euxassay_011943_22	euxassay_011943_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18144_wholemount_strong.wlz

18144_wholemount_moderate.wlz

18144_wholemount_weak.wlz

18144_wholemount_possible.wlz

18144_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18144_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 14 15 16 17 18 19 20

humerus

moderate

homogeneous

moderate expression: see section 19 20 21 22 23

fibula

moderate

homogeneous

moderate expression: see section 22

tibia

moderate

homogeneous

moderate expression: see section 22

femur

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 15 16 17 18 19 20

naris

moderate

homogeneous

moderate expression: see section 11

nasal septum

moderate

homogeneous

moderate expression: see section 14

viscerocranium

moderate

homogeneous

Expression in the turbinate bone.

meckel's cartilage

moderate

homogeneous

moderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

axial skeleton

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 12 13 14

basioccipital bone

moderate

homogeneous

moderate expression: see section 01 02 03 15 16 17 18 19 20

basisphenoid bone

moderate

homogeneous

moderate expression: see section 07 08 09 10 11 12 13 14

temporal bone petrous part

moderate

homogeneous

moderate expression: see section 01 20

vault of skull

moderate

homogeneous

moderate expression: see section 01

orbito-sphenoid

moderate

homogeneous

moderate expression: see section 01 02 04 05 07 18 19 21 22

scapula

moderate

homogeneous

moderate expression: see section 18 19 20

pelvic girdle skeleton

moderate

homogeneous

moderate expression: see section 06 07 13

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30014
Entity Detected:	Adrm1, adhesion regulating molecule 1 ( MGI:1929289)
Sequence:	sense strand is shown >T30014 GTTCCGGGCAGGAAAAATGTCATTAAAAGGAACTACGGTCACCCCAGATAAACGGAAAGGTCTCGTGTAC ATCCAGCAGACGGACGACTCCCTTATTCACTTCTGTTGGAAAGACAGGACCTCTGGGACCGTGGAGGATG ACTTGATTATCTTTCCTGATGACTGTGAGTTCAAGCGGGTACCTCAGTGCCCCAGTGGGAGGGTCTACGT GCTCAAGTTTAAGGCAGGGTCCAAGCGGCTCTTCTTCTGGATGCAGGAGCCCAAGACTGACCAAGATGAG GAGCACTGCCGGAAAGTCAACGAGTGTCTGAACAACCCCCCCATGCCTGGGTCACTGGGAGCAAGTGGGA GTAGTGGCCATGAGCTTTCAGCACTGGGCGGTGAGGGTGGCCTGCAGAGCCTGTTGGGGAACATGAGTCA CAGCCAGCTTATGCAGCTCATCGGACCAGCCGGCCTGGGAGGACTGGGTGGACTTGGGGCCCTCACTGGG CCAGGCCTGGCCAGCTTGCTGGGGAGCAGTGGACCTCCAGCCAGCAGCTCTTCATCCAGCTCCCGGAGCC AGTCGGCAGCCGTCACCCCATCCTCCTCCACCTCTTCCGCTCGCGCCACCCCAGCCCCTTCTGCCCCAGC AGCTGCCTCGGCAACCAGCCCAAGCCCCGCACCCAGCTCAGGTAATGGAACCAGCACAGCAGCCAGCCCG ACCCAGCCCATCCAGCTGAGCGACCTCCAGAGCATTCTGGCCACTATGAACGTGCCGGCAGGGCCAGGAG GCAGCCAGCAAGTGGATCTGGCGAGTGTGCTGACCCCAGAGATCATGGCTCCCATCCTTGCCAAT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3586698), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 34827. Forward Primer - name:034827_F_IRAV12-15_F05_Adrm1, sequence:GTTCCGGGCAGGAAAAAT; Reverse Primer - name:034827_R_SP6_IRAV12-15_F05_Adrm1, sequence:CATTGGCAAGGATGGGAG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18142 same embryo
	EMAGE:18143 same embryo
	EMAGE:18141 same embryo
	EMAGE:18145 same embryo
	EurExpress:euxassay_011943 same experiment
	MGI:4823017 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source