EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:18204

Kdm5a lysine (K)-specific demethylase 5A ( MGI:2136980)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011985. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011985_01	euxassay_011985_02	euxassay_011985_03	euxassay_011985_04

euxassay_011985_05	euxassay_011985_06	euxassay_011985_07	euxassay_011985_08	euxassay_011985_09

euxassay_011985_10	euxassay_011985_11	euxassay_011985_12	euxassay_011985_13	euxassay_011985_14

euxassay_011985_15	euxassay_011985_16	euxassay_011985_17	euxassay_011985_18	euxassay_011985_19

euxassay_011985_20	euxassay_011985_21	euxassay_011985_22	euxassay_011985_23	euxassay_011985_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18204_wholemount_strong.wlz

18204_wholemount_moderate.wlz

18204_wholemount_weak.wlz

18204_wholemount_possible.wlz

18204_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18204_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

strong

regional

strong expression: see section 05 06 07 08 weak expression: see section 15 16 17

vibrissa

strong

regional

strong expression: see section 04 05 06 07 moderate expression: see section 08 21 22 23

cornea epithelium

strong

regional

strong expression: see section 03 04

oral epithelium

strong

regional

strong expression: see section 05

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30061
Entity Detected:	Kdm5a, lysine (K)-specific demethylase 5A ( MGI:2136980)
Sequence:	sense strand is shown >T30061 ATTTGTGCTCTTGCCCCATGCAGAACAAATGTCTTAGGTACCGCTATCCACTAGAAGACCTTCCATCTCT GCTTTATGGTGTCAAAGTGAGAGCACAATCCTATGACACTTGGGTCAATCGTGTTACAGAAGCATTGTCA GCTAGCTTCAACCATAAGAAAGATTTGATTGAATTGAGAGTAATGCTGGAAGATGCTGAGGATAGGAAAT ACCCAGAGAATGATCTCTTTCGAAAGCTCAGGGACGCGGTGAAAGAAGCTGAGACCTGTGGTTCTGTGGC TCAGCTGCTTCTGAGCAAAAAGCAAAAACACAGAATCTGCTGGATGATGTAGAAGAATTTCATGAACGTG CCCAGGAGGCCATGATGGATGAAACCCCAGATTCTTCCAAACTCCAAATGTTAATAGACATGGGCTCTAG TCTCTATGTGGAGCTACCTGAATTGCCCCGACTAAAGCAAGAGCTCCAGCAGGCTCGATGGCTGGATGAA GTAAGGCTAACTCTATCAGATCCACAACAAGTCACTTTGGATGTCATGAAGAAATTGATTGACTCTGGCG TAGGCTTGGCACCTCACCATGCTGTAGAAAAAGCAATGGCCGAACTACAGGAACTCCTTACAGTCTCTGA ACGATGGGAAGAGAAGGCTAAGGTCTGCCTGCAAGCAAGACCTCGGCACAGTATGGCAAATTTAGAAAAT ATTGTGAATGAAGCCAAGAACATTCCAGCCTTTCTACCCAATGTGTTGTCCTTAAAAGAAGCCTTACAAA AGGCTCGTGAATGGACAGCTAAAGTGGAAGCTATTCAGAGTGGCAACAACTATGCTTACTTGGAACAGCT TGAGAGCCTATCTGCTAAAGGACGCCCTATCCCTGTGCGTCTTGATGCCTTGCCTCAAGTGGAGTCACAG GTAGCAGCAGCACGGGCCTGGAGAGAACGTACTGGGCGGACCTTCCTTAA
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3603073), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 15690. Forward Primer - name:015690_F_IRAV20-23_C08_Jarid1a, sequence:ATTTGTGCTCTTGCCCCA; Reverse Primer - name:015690_R_SP6_IRAV20-23_C08_Jarid1a, sequence:TTTAAGGAAGGTCCGCCC. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18201 same embryo
	EMAGE:18203 same embryo
	EMAGE:18200 same embryo
	EMAGE:18205 same embryo
	EMAGE:18202 same embryo
	EurExpress:euxassay_011985 same experiment
	MGI:4825746 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source