EMAGE mouse gene expression spatial database

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EMAGE:18644

Frem2 Fras1 related extracellular matrix protein 2 ( MGI:2444465)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_016540. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_016540_01	euxassay_016540_02	euxassay_016540_03	euxassay_016540_04

euxassay_016540_05	euxassay_016540_06	euxassay_016540_07	euxassay_016540_08	euxassay_016540_09

euxassay_016540_10	euxassay_016540_11	euxassay_016540_12	euxassay_016540_13	euxassay_016540_14

euxassay_016540_15	euxassay_016540_16	euxassay_016540_17	euxassay_016540_18	euxassay_016540_19

euxassay_016540_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18644_wholemount_strong.wlz

18644_wholemount_moderate.wlz

18644_wholemount_weak.wlz

18644_wholemount_possible.wlz

18644_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18644_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diaphragm

weak

regional

weak expression: see section 15 16

pericardial cavity

weak

regional

weak expression: see section 02 03 04 05 14 15 16

peritoneal component

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14

pleural cavity

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16

vertebral axis musculature

moderate

regional

moderate expression: see section 01 02 03 04 13 14 15 16 weak expression: see section 05 06 07 08 09 10 17 18 19 20

thymus primordium

weak

regional

weak expression: see section 09 10 11 12

vibrissa

moderate

regional

moderate expression: see section 04 05 19 weak expression: see section 06 07 08 17 18

diencephalon floor plate

moderate

regional

moderate expression: see section 12 weak expression: see section 13

lateral ventricle choroid plexus

weak

regional

weak expression: see section 07 08 09 10 11 13 14

medulla oblongata basal plate mantle layer

strong

single cell

strong expression: see section 10 11 12 13 moderate expression: see section 09

metencephalon floor plate

strong

regional

strong expression: see section 10 moderate expression: see section 11

pons mantle layer

strong

single cell

strong expression: see section 10 11 12

metencephalon part of 4th ventricle choroid plexus

weak

regional

weak expression: see section 09 10 11 12 13 14 15

spinal cord floor plate

moderate

regional

moderate expression: see section 11

spinal cord lateral wall

moderate

regional

moderate expression: see section 12

ventral grey horn

strong

single cell

strong expression: see section 10 11 12 moderate expression: see section 09

saccule

strong

regional

strong expression: see section 05 06 07 08 15 16

pharyngo-tympanic tube

weak

regional

weak expression: see section 01 02 03 17 18 19

cornea epithelium

moderate

regional

moderate expression: see section 04 05 18 19 20 weak expression: see section 01 02 03

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 11 12 13 14 15 16 weak expression: see section 10

esophagus

weak

regional

weak expression: see section 10 11

tongue muscle

weak

regional

weak expression: see section 11 12 13 14 15

stomach

strong

regional

strong expression: see section 01 02 03 moderate expression: see section 04 weak expression: see section 05 06 07 08

lower jaw incisor

moderate

regional

moderate expression: see section 10 11 14 15 16

upper jaw incisor

moderate

regional

moderate expression: see section 11 12 15 16

metanephros

strong

regional

strong expression: see section 05 06 07 08 14 15 16

lung

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16

trachea

weak

regional

weak expression: see section 11

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T38972
Entity Detected:	Frem2, Fras1 related extracellular matrix protein 2 ( MGI:2444465)
Sequence:	sense strand is shown >T38972 ATGTGACCATCCTCACAGACAGGGATGATGAGCCTGCCTTCTACTTTGGGGACACCCAGTACTCCGTGGA TGAGAGTGCCGGCTACGTGGAGCTGCAGGTGTGGAGGACGGGGACCGACCTCTCTAAGCCTTCCAGTGTC ACAGTGAGGTCCCGGAAAACAGAATCCCTCTCTGCAGATGCTGGAACAGACTATGTGGGAATCAGTCGCA ATTTAGATTTTGCGCCTGGCGTCAACATGCAGACTGTTCGAGTGGTCATTCTGGATGACCTTGGACGACC AATACTGGAGGGAATTGAGAAGTTTGAACTGGTACTTCGAATGCCTATGAACGCTGCCCTTGGGGAGCCC AGCAAGGCCACCGTGTCCATAAATGACTCTGCCTCAGACTTGCCTAAAATGCAATTCAAAGAGAGAGTGT ACACTTGCAACGAGAATGACGGGCGTGTAGTGGCCATGATCTACAGGAGCGGTGACATCCAGCACAGGTC TTCTGTGAGATGTTACACGAGGCAGGGGTCTGCGCAGGTGATGATGGACTTCGAGGAACGCCCAAATAC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 264922. Forward Primer - name:264922_F_cDNA_Frem2, sequence:ATGTGACCATCCTCACAGACAG; Reverse Primer - name:264922_N_SP6_cDNA_Frem2, sequence:GTATTTGGGCGTTCCTCGAA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18646 same embryo
	EMAGE:18641 same embryo
	EMAGE:18643 same embryo
	EMAGE:18645 same embryo
	EMAGE:18642 same embryo
	EurExpress:euxassay_016540 same experiment
	MGI:4824921 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source