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EMAGE:18812

Wdr96 WD repeat domain 96 ( MGI:1289258)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:18812 EMAGE:18812 EMAGE:18812 EMAGE:18812 EMAGE:18812
"Pseudo-wholemount" of euxassay_014039. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_014039_01 euxassay_014039_02 euxassay_014039_03 euxassay_014039_04
EMAGE:18812 EMAGE:18812 EMAGE:18812 EMAGE:18812 EMAGE:18812
euxassay_014039_05 euxassay_014039_06 euxassay_014039_07 euxassay_014039_08 euxassay_014039_09
EMAGE:18812 EMAGE:18812 EMAGE:18812 EMAGE:18812 EMAGE:18812
euxassay_014039_10 euxassay_014039_11 euxassay_014039_12 euxassay_014039_13 euxassay_014039_14
EMAGE:18812 EMAGE:18812 EMAGE:18812 EMAGE:18812 EMAGE:18812
euxassay_014039_15 euxassay_014039_16 euxassay_014039_17 euxassay_014039_18 euxassay_014039_19
EMAGE:18812 EMAGE:18812 EMAGE:18812 EMAGE:18812
euxassay_014039_20 euxassay_014039_21 euxassay_014039_22 euxassay_014039_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:18812Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
18812_wholemount_strong.wlz
18812_wholemount_moderate.wlz
18812_wholemount_weak.wlz
18812_wholemount_possible.wlz
18812_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:18812_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
lateral ventricle choroid plexus
strong strong
regionalstrong expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16
metencephalon part of 4th ventricle choroid plexus
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T40223
Entity Detected:Wdr96, WD repeat domain 96 ( MGI:1289258)
Sequence:sense strand is shown

>T40223
GAAAGGAGAGTGAAGGCAGAAATGGTCATCAACCAGGAGGAACTGAAAATAAACAACATTATATTTTCGT
TGCTGCTGGATGAAGAACTCAGCTCCAGGGAACAGTTCCTGAACAACTACCTTTTGAAGAAACAGGAGGA
GAAAACCAAGACTGCAGAAGCCATCCAGAAGGCGAGAGAAGATCTGGACGTATTCAAGGAGCACCATGAC
ATGTTAGTGGCAGAGGACAAAATTCTGGATCGAAGCTTCAAAAAGGAATTTTCTGACATTTTGGGTCACC
AGGTGGATGTCCTCTACAAACTTTTTAAACGCCGGCCAAGGGTTCACAAACAGAAAACACAGGCAGATGT
GACCAGTCTTGTTCCTTATGGAGAGCGACCAGGATCTGCCAAGTTAAATAAGGAAAACTTAGCCCAGCTA
ATGAAATCCATGGATGAGTTGGACAATATCAATAACATGCCAGAAGGCTTGGACCCCTCTGTGAAGCAGA
AGGCAGCCTGCCTCCTGGAAATGACGACTTTTCTCCGGAAGCGGATGGAAGAGGATGACGTAGTGCATCA
TGAGATCGAAAAAGTGTTCCATGAGCTCATCCGATTACAGGATGAGAAAGTGAGATTCCAGGTGAATTTG
ACAGTACAAATCCTTCTTAAGCAAGGACAAGTAGAGCTGGAGAATTTCCAGTTGATGCTGGAATATTCTG
ACGCAATTCTCATCAATAAGAATATAATTGAAGACTTGAATTCTGTGATTCGGACTCAAGGACAAAAGAA
AGTTGCTAGCATGATGGAAAGTAAAGAGGTCCACAAAGGAATCTATCAGATTGAGTGGGAGCACAAGAAG
ATGGAGATGGAGATGGAGGACCTAAACCAGAGGG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 86176. Forward Primer - name:086176_F_cDNA_D19Ertd652e, sequence:GAAAGGAGAGTGAAGGCAGAAA; Reverse Primer - name:086176_N_SP6_cDNA_D19Ertd652e, sequence:CCCTCTGGTTTAGGTCCTCCA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:18813 same embryo
 EMAGE:18809 same embryo
 EMAGE:18808 same embryo
 EMAGE:18810 same embryo
 EMAGE:18811 same embryo
 EurExpress:euxassay_014039 same experiment
 MGI:4824200 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS