EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:18824

Rapgef4 Rap guanine nucleotide exchange factor (GEF) 4 ( MGI:1917723)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_015802. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_015802_01	euxassay_015802_02	euxassay_015802_03	euxassay_015802_04

euxassay_015802_05	euxassay_015802_06	euxassay_015802_07	euxassay_015802_08	euxassay_015802_09

euxassay_015802_10	euxassay_015802_11	euxassay_015802_12	euxassay_015802_13	euxassay_015802_14

euxassay_015802_15	euxassay_015802_16	euxassay_015802_17	euxassay_015802_18	euxassay_015802_19

euxassay_015802_20	euxassay_015802_21	euxassay_015802_22	euxassay_015802_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18824_wholemount_strong.wlz

18824_wholemount_moderate.wlz

18824_wholemount_weak.wlz

18824_wholemount_possible.wlz

18824_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18824_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

adrenal gland

strong

regional

strong expression: see section 05 14 15 moderate expression: see section 06 07 08 16

facial vii ganglion

strong

regional

strong expression: see section 05 19 20

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 06 07 17 18

trigeminal v ganglion

strong

regional

strong expression: see section 04 05 06 19 20 21 moderate expression: see section 03 07 08 09 17 18

dorsal root ganglion

moderate

regional

moderate expression: see section 06 07 08 13 14 15 weak expression: see section 12 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T40152
Entity Detected:	Rapgef4, Rap guanine nucleotide exchange factor (GEF) 4 ( MGI:1917723)
Sequence:	sense strand is shown >T40152 CATTACCAAGGTCCCTTCAGAGAAGATCCTCAGAGCTGGAAAAATTTTACGAATTGCCATTCTCTCTCGA GCTCCCCACATGATAAGAGACAGAAAGTACCACCTAAAGACATACAGACAATGCTGTGTTGGGACTGAGC TGGTAGACTGGATGATACAGCAGACATCCTGTGTTCACTCGCGGACTCAAGCTGTTGGCATGTGGCAAGT CTTGCTGGAAGATGGTGTCCTCAACCATGTGGACCAGGAGCGCCATTTCCAAGACAAATATTTATTTTAT CGATTTCTGGATGACGAGCGTGAGGATGCCCCTTTGCCTACTGAGGAAGAGAAGAAGGAGTGTGATGAAG AACTTCAGGACACCATGCTGCTGCTCTCACAGATGGGCCCTGACGCCCACATGAGAATGATCCTGCGAAA ACCACCTGGCCAGAGGACTGTGGATGACCTAGAGATTATCTACGACGAGCTCCTTCATATTAAAGCCTTA TCCCATCTCTCTACCACAGTGAAACGGGAGTTAGCAGGTGTTCTCATTTTTGAGTCTCACGCCAAAGGAG GAACTGTGTTGTTTAACCAGGGGGAAGAAGGTACCTCCTGGTACATCATTCTGAAAGGATCCGTGAATGT AGTCATTTATGGCAAGGGTGTGGTCTGCACCCTGCACGAAGGAGATGACTTTGGCAAGTTAGCTCTAGTG AACGATGCTCCAAGAGCTGCCTCCATTGTTCTTCGGGAAGATAATTGTCACTTCCTAAGAGTCGACAAGG AAGACTTCAATCGGATTCTGAGGGACGTTGAGGCGAATACAGTCAGACTTAAAGAACATGACCAAGATGT CTTGGTACTGGAGAAGGTCCCAGCAGGGAACAGAGCTGCTAATCAAGGAAACTCACAGCCTCAGCAAAAG TATAC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 161047. Forward Primer - name:161047_F_cDNA_mCG128278.1, sequence:CATTACCAAGGTCCCTTCAGAG; Reverse Primer - name:161047_N_SP6_cDNA_mCG128278.1, sequence:GTATACTTTTGCTGAGGCTGTGA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18819 same embryo
	EMAGE:18823 same embryo
	EMAGE:18820 same embryo
	EMAGE:18822 same embryo
	EMAGE:18821 same embryo
	EurExpress:euxassay_015802 same experiment
	MGI:4827613 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source