EMAGE mouse gene expression spatial database

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EMAGE:18997

Ece1 endothelin converting enzyme 1 ( MGI:1101357)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_015924. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_015924_01	euxassay_015924_02	euxassay_015924_03	euxassay_015924_04

euxassay_015924_05	euxassay_015924_06	euxassay_015924_07	euxassay_015924_08	euxassay_015924_09

euxassay_015924_10	euxassay_015924_11	euxassay_015924_12	euxassay_015924_13	euxassay_015924_14

euxassay_015924_15	euxassay_015924_16	euxassay_015924_17	euxassay_015924_18	euxassay_015924_19

euxassay_015924_20	euxassay_015924_21	euxassay_015924_22	euxassay_015924_23	euxassay_015924_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18997_wholemount_strong.wlz

18997_wholemount_moderate.wlz

18997_wholemount_weak.wlz

18997_wholemount_possible.wlz

18997_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18997_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

weak

regional

weak expression: see section 07 08 09 10 16 17 18 19

thyroid gland

weak

regional

weak expression: see section 09 10 13 14

pituitary gland

weak

regional

weak expression: see section 10 11 12 13 14 15

vibrissa

weak

regional

weak expression: see section 06 07 23 24

pharyngo-tympanic tube

moderate

regional

moderate expression: see section 04 05 06 07 08 17 18 19 20 weak expression: see section 01 02 03 21 22 23

cornea

weak

regional

weak expression: see section 04 05 24

naris

moderate

regional

moderate expression: see section 14 15 17 18

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 11 12 13 14 16 17 18 weak expression: see section 10 19 20

esophagus

weak

regional

weak expression: see section 11 12

oral epithelium

moderate

regional

moderate expression: see section 06 07 08 09 10 13 14 15 16 17 21 22 weak expression: see section 11 12

larynx

moderate

regional

moderate expression: see section 13 weak expression: see section 11 12

trachea

weak

regional

weak expression: see section 12

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T63373
Entity Detected:	Ece1, endothelin converting enzyme 1 ( MGI:1101357)
Sequence:	sense strand is shown >T63373 GATGGGTTGATACCCAAGTGATAGTTGAGGCAGCACAAAGGCAGAGTAATGACCTTCATATCACGTGGCA CCCGAACACTCTAGACTAGAACATAGGAGCCTGTTGGAGCTGAGAACCCACACCCCACCAGATGCACCCT GTTGTCCAAAGATGTCTTTTTTTTTTTTTTTTTTGGTCCCACCTCTTCTGGCCCTGAGACCCGTTGCCTC TCTGTAGCAATTCTGACATCCTGAAGTGGTCAGCTTCACTGATCCTCTGTGCAGAGGGAAAGGGGAAAAA AAACTCAGCCCAGTTCTCCCTCCAAGCTCTGTAGCCTGTAGTTGCCCTGGCCTGGCTCCTGGGACCCCCT TCTCTAGTGCCTTACCCCAGGCTGCAGACCCTGAGCCCCACTAGGAGGCAGCATTTGGCTTGCTTTCCCA GTGGAGCCCCCAAGTGTCCTGACTAGAAGCCAACACCATAGCCCCATTCCCAGAAGCCCCAGGGTACCAC CCCAACCTGCCTACAGGACAGCCATTCCAGATAGACGTCCAGTGTCAGGAGCTGCTGACCTCTCAGGCTT TCAGCCACCCGTGGCACGCACATTGAATCAATTCAGTCCTTGTAGATAGAGGGATTCCCCTCACCCCCTC CCCCTCTCTGTTTTTTTGGAGGTGGATGCTGTAGCCAGAGCAGGCCAAGACTGGAATGTTCCCAGCCCCA TTGGGCTCCAGGGAATGGCAGAAAGTCATCCACCCACAGCATCACGTAGTAGATATGTGGGCTCCCACTA GGTGGCGCTCACCGCAGTTCTCATGACTCCTGCAGGGAG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 96389. Forward Primer - name:096389_F_cDNA_Ece1, sequence:GATGGGTTGATACCCAAGTGAT; Reverse Primer - name:096389_N_SP6_cDNA_Ece1, sequence:CTCCCTGCAGGAGTCATGAGA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18998 same embryo
	EMAGE:18993 same embryo
	EMAGE:18996 same embryo
	EMAGE:18995 same embryo
	EMAGE:18994 same embryo
	EurExpress:euxassay_015924 same experiment
	MGI:4824461 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source