EMAGE mouse gene expression spatial database

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EMAGE:19062

Eif4g3 eukaryotic translation initiation factor 4 gamma, 3 ( MGI:1923935)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_015952. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_015952_01	euxassay_015952_02	euxassay_015952_03	euxassay_015952_04

euxassay_015952_05	euxassay_015952_06	euxassay_015952_07	euxassay_015952_08	euxassay_015952_09

euxassay_015952_10	euxassay_015952_11	euxassay_015952_12	euxassay_015952_13	euxassay_015952_14

euxassay_015952_15	euxassay_015952_16	euxassay_015952_17	euxassay_015952_18	euxassay_015952_19

euxassay_015952_20	euxassay_015952_21	euxassay_015952_22	euxassay_015952_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19062_wholemount_strong.wlz

19062_wholemount_moderate.wlz

19062_wholemount_weak.wlz

19062_wholemount_possible.wlz

19062_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19062_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon

weak

regional

weak expression: see section 06 07 08 09 10 11 12 13 14 15 16

telencephalon

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

hindbrain

weak

regional

weak expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

midbrain

weak

regional

weak expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18

facial vii ganglion

weak

regional

weak expression: see section 03 04 18 19 20

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 06 07 16 17

trigeminal v ganglion

weak

regional

weak expression: see section 02 03 04 05 06 07 16 17 18 19 20

vestibulocochlear viii ganglion

weak

regional

weak expression: see section 06 16 17

trigeminal v nerve

weak

regional

weak expression: see section 15

spinal cord

weak

regional

weak expression: see section 10 11 12 13 14 15 16

dorsal root ganglion

weak

regional

weak expression: see section 07 08 09 10 11 12 13 16 17

neural retina

weak

regional

weak expression: see section 01 21 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T63305
Entity Detected:	Eif4g3, eukaryotic translation initiation factor 4 gamma, 3 ( MGI:1923935)
Sequence:	sense strand is shown >T63305 CAGAAGAGGAAGTGGAGAGGAAGTCCAAGTCTATCATTGACGAATTCTTACACATCAATGACTTTAAGGA GGCCACGCAGTGCATAGAGGAGCTGAGCGCCCAGGGCCCACTGCATGTGTTCGTGAAGGTGGGTGTGGAG TTCACCCTGGAACGGAGCCAGATCACCAGGGACCACATGGGCCACTTACTGTATCAGCTGGTGCAGTCAG AAAAACTCAGCAAGCAGGACTTTTTCAAAGGTTTTTCTGAAACCTTGGAGTTGGCAGATGACATGGCCAT TGATATTCCCCATATTTGGTTGTACCTGGCTGAACTGGTCACCCCCATGTTAAAAGGAGGGGGGATTTCC ATGAGAGAACTTATTGTGGAATTCAGCAAGCCATTACTTCCTGTTGGCAGAGCTGGGGTCCTGCTTTCTG AAATCCTACACCTTCTATGCAGACAAATGAGCCATAAGAAAGTAGGCGCCCTGTGGAGGGAGGCTGACCT CAGCTGGAAGGACTTTTTACCGGAAGGGGAAGATGTCCATCATTTTCTCTTGGAGCAGAAGTTGGACTTC ACGGAATCCGAAGGGCCCTGCTCCTCTGAGGCACTGTCAAAGAAGGAGCTGTCTGCCGAGGAGCTGTCTC AGCGCCTGGAAAAGCTCATCATGGAGGAGAAAGCGGATGACGAGCGGATCTTTGACT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 88601. Forward Primer - name:088601_F_cDNA_Eif4g3, sequence:CAGAAGAGGAAGTGGAGAGGAA; Reverse Primer - name:088601_N_SP6_cDNA_Eif4g3, sequence:AGTCAAAGATCCGCTCGTCAT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19063 same embryo
	EMAGE:19064 same embryo
	EMAGE:19065 same embryo
	EMAGE:19066 same embryo
	EMAGE:19067 same embryo
	EurExpress:euxassay_015952 same experiment
	MGI:4824515 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source